| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pcDNA3.1/GS , pcDNA3.1GS |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 启动子: | CMV |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4020 bp |
| 5' 测序引物: | pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
| 3' 测序引物: | pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
| 载体标签: | C-His, C-V5-Epitope |
| 载体抗性: | Ampicillin 氨苄 |
| 筛选标记: | Neomycin (G418) |
| 备注: |
|
| 产品目录号: | H-K1000 |
| 稳定性: | 瞬表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
买家导航
pcDNA3.1/GS载体 pcDNA3.1GS质粒基本信息
pcDNA3.1/GS质粒图谱 pcDNA3.1GS载体图谱和pcDNA3.1/GS载体序列 pcDNA3.1GS质粒序列多克隆位点信息
pcDNA3.1/GS质粒 pcDNA3.1GS载体简介
GeneStorm Expression-Ready Clones are expressed from the vector pcDN3.1/GS. This vector utilizes the strong immediate-early cytomegalovirus (CMV) promoter for high-level, constitutive expression in mammalian cell lines. Each GeneStorm Clone is fused to a C-terminal peptide encoding the V5 epitope for detection with the Anti-V5-HRP Antibody and a 6His tag for purification on metal-chelating resin (i.e. ProBond). The vector also encodes the Zeocin resistance gene for selection in E. coli and for the creation of stable mammalian cell lines.
pcDNA3.1/GS质粒序列 pcDNA3.1GS载体序列
LOCUS pcDNA3.1/GS 4020 bp DNA circular SYN 2-SEP-2003
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4020
/invitrogen="138"
/vntifkey="98"
CDS 82..99
/invitrogen="1010000"
/vntifkey="4"
/label=6xHis
polyA_site 128..352
/invitrogen="1870000"
/vntifkey="26"
/label=BGH\pA
primer complement(122..139)
/invitrogen="1910000"
/vntifkey="27"
/label=BGH\reverse\primer
primer 3876..3896
/invitrogen="1930000"
/vntifkey="27"
/label=CMV\forward\primer
promoter 3339..3926
/invitrogen="2180000"
/vntifkey="29"
/label=CMV\promoter
promoter 1216..1283
/invitrogen="2340000"
/vntifkey="30"
/label=EM7\promoter
rep_origin 398..826
/invitrogen="2500000"
/vntifkey="33"
/label=f1\origin
rep_origin complement(2301..2974)
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
promoter 831..1201
/invitrogen="2290000"
/vntifkey="29"
/label=SV40\early\promoter
polyA_signal 1788..1918
/invitrogen="1840000"
/vntifkey="25"
/label=SV40\pA
primer 3970..3989
/invitrogen="2100000"
/vntifkey="27"
/label=T7\primer
promoter 3970..3986
/invitrogen="2370000"
/vntifkey="30"
/label=T7\promoter
protein_bind 4016..4020
/invitrogen="2460000"
/vntifkey="31"
/label=TOPO\binding\site
protein_bind complement(1..5)
/invitrogen="2460000"
/vntifkey="31"
/label=TOPO\binding\site
primer complement(40..60)
/invitrogen="2140000"
/vntifkey="27"
/label=V5\reverse\primer
CDS 31..72
/invitrogen="1350000"
/vntifkey="4"
/label=V5\epitope
CDS 1284..1658
/invitrogen="1370000"
/vntifkey="4"
/label=Zeo(R)
BASE COUNT 905 a 1068 c 1049 g 998 t
ORIGIN
1 aagggcgagc ttcgaggtca cccattcgaa ggtaagccta tccctaaccc tctcctcggt
61 ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
121 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
181 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
241 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
301 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
361 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
541 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc
601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc
781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
841 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
901 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
961 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
1021 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
1081 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
1141 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
1201 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
1261 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
1321 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
1381 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
1441 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
1501 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
1561 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
1621 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
1681 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
1741 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
1801 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
1861 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
1921 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
1981 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
2041 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
2101 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
2161 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
2221 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
2281 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
2341 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
2401 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
2461 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
2521 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
2581 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
2641 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
2701 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
2761 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
2821 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
2881 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
2941 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
3001 cacgttaagg gattttggtc atgagcggat acatatttga atgtatttag aaaaataaac
3061 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
3121 atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag
3181 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
3241 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
3301 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
3361 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
3421 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
3481 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
3541 ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
3601 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
3661 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
3721 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
3781 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
3841 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
3901 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
3961 tatcgaaatt aatacgactc actataggga gacccaagct ggctagttaa gctcgccctt
//
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4020
/invitrogen="138"
/vntifkey="98"
CDS 82..99
/invitrogen="1010000"
/vntifkey="4"
/label=6xHis
polyA_site 128..352
/invitrogen="1870000"
/vntifkey="26"
/label=BGH\pA
primer complement(122..139)
/invitrogen="1910000"
/vntifkey="27"
/label=BGH\reverse\primer
primer 3876..3896
/invitrogen="1930000"
/vntifkey="27"
/label=CMV\forward\primer
promoter 3339..3926
/invitrogen="2180000"
/vntifkey="29"
/label=CMV\promoter
promoter 1216..1283
/invitrogen="2340000"
/vntifkey="30"
/label=EM7\promoter
rep_origin 398..826
/invitrogen="2500000"
/vntifkey="33"
/label=f1\origin
rep_origin complement(2301..2974)
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
promoter 831..1201
/invitrogen="2290000"
/vntifkey="29"
/label=SV40\early\promoter
polyA_signal 1788..1918
/invitrogen="1840000"
/vntifkey="25"
/label=SV40\pA
primer 3970..3989
/invitrogen="2100000"
/vntifkey="27"
/label=T7\primer
promoter 3970..3986
/invitrogen="2370000"
/vntifkey="30"
/label=T7\promoter
protein_bind 4016..4020
/invitrogen="2460000"
/vntifkey="31"
/label=TOPO\binding\site
protein_bind complement(1..5)
/invitrogen="2460000"
/vntifkey="31"
/label=TOPO\binding\site
primer complement(40..60)
/invitrogen="2140000"
/vntifkey="27"
/label=V5\reverse\primer
CDS 31..72
/invitrogen="1350000"
/vntifkey="4"
/label=V5\epitope
CDS 1284..1658
/invitrogen="1370000"
/vntifkey="4"
/label=Zeo(R)
BASE COUNT 905 a 1068 c 1049 g 998 t
ORIGIN
1 aagggcgagc ttcgaggtca cccattcgaa ggtaagccta tccctaaccc tctcctcggt
61 ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
121 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
181 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
241 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
301 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
361 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
541 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc
601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc
781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
841 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
901 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
961 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
1021 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
1081 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
1141 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
1201 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
1261 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
1321 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
1381 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
1441 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
1501 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
1561 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
1621 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
1681 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
1741 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
1801 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
1861 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
1921 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
1981 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
2041 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
2101 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
2161 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
2221 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
2281 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
2341 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
2401 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
2461 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
2521 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
2581 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
2641 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
2701 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
2761 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
2821 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
2881 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
2941 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
3001 cacgttaagg gattttggtc atgagcggat acatatttga atgtatttag aaaaataaac
3061 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
3121 atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag
3181 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
3241 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
3301 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
3361 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
3421 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
3481 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
3541 ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
3601 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
3661 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
3721 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
3781 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
3841 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
3901 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
3961 tatcgaaatt aatacgactc actataggga gacccaagct ggctagttaa gctcgccctt
//
pcDNA3.1/GS载体图谱 pcDNA3.1GS质粒图谱pdf版和pcDNA3.1/GS载体序列 pcDNA3.1GS质粒序列等相关资料下载
pcDNA3.1/GS质粒 pcDNA3.1GS载体应用举例
- 上一篇：pcDNA3.1/His C
- 下一篇：pcDNA3.1/Myc-His A
