| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pcDNA3.1/Myc-His A , pcDNA3.1/Myc-HisA |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 启动子: | CMV |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5494 bp |
| 5' 测序引物: | pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
| 3' 测序引物: | pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
| 载体标签: | C-His, C-Myc |
| 载体抗性: | Ampicillin 氨苄 |
| 筛选标记: | Neomycin (G418) |
| 备注: |
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| 产品目录号: | V855-20 |
| 稳定性: | 瞬表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
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pcDNA3.1/Myc-His A载体 pcDNA3.1/Myc-HisA质粒基本信息
pcDNA3.1/Myc-His A质粒图谱 pcDNA3.1/Myc-HisA载体图谱和pcDNA3.1/Myc-His A载体序列 pcDNA3.1/Myc-HisA质粒序列多克隆位点信息
pcDNA3.1/Myc-His A质粒 pcDNA3.1/Myc-HisA载体简介
pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
pcDNA3.1/Myc-His A质粒序列 pcDNA3.1/Myc-HisA载体序列
LOCUS pcDNA3.1(+)/myc-His A 5494 bp DNA circular SYN SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..5494 /invitrogen="126" /vntifkey="98" CDS 1042..1059 /invitrogen="1010000" /vntifkey="4" /label=6xHis CDS complement(4498..5358) /invitrogen="1020000" /vntifkey="4" /label=Amp(R) polyA_site 1088..1312 /invitrogen="1870000" /vntifkey="26" /label=BGH\pA primer complement(1082..1099) /invitrogen="1910000" /vntifkey="27" /label=BGH\reverse\primer promoter complement(5359..5457) /invitrogen="2330000" /vntifkey="30" /label=bla\promoter primer 769..789 /invitrogen="1930000" /vntifkey="27" /label=CMV\forward\primer promoter 232..819 /invitrogen="2180000" /vntifkey="29" /label=CMV\promoter CDS 997..1026 /invitrogen="1090000" /vntifkey="4" /label=c-myc\epitope rep_origin 1358..1786 /invitrogen="2500000" /vntifkey="33" /label=f1\origin misc_feature 902..999 /invitrogen="1490000" /vntifkey="21" /label=MCS CDS 2197..2991 /invitrogen="1260000" /vntifkey="4" /label=Neo(R) rep_origin complement(3680..4353) /invitrogen="2540000" /vntifkey="33" /label=pUC\origin promoter 1791..2161 /invitrogen="2290000" /vntifkey="29" /label=SV40\early\promoter polyA_signal 3167..3297 /invitrogen="1840000" /vntifkey="25" /label=SV40\pA primer 863..882 /invitrogen="2100000" /vntifkey="27" /label=T7\primer promoter 863..879 /invitrogen="2370000" /vntifkey="30" /label=T7\promoter BASE COUNT 1273 a 1427 c 1400 g 1394 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca 961 gcacagtggc ggccgctcga gtctagaggg cccttcgaac aaaaactcat ctcagaagag 1021 gatctgaata tgcataccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca 1081 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 1141 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 1201 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 1261 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 1321 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 1381 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 1441 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 1501 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc 1561 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 1621 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 1681 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc 1741 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 1801 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc 1861 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 1921 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 1981 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 2041 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 2101 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 2161 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 2221 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 2281 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 2341 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg 2401 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 2461 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 2521 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 2581 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 2641 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 2701 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat 2761 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 2821 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 2881 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 2941 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 3001 tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 3061 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 3121 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 3181 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 3241 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 3301 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 3361 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 3421 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 3481 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 3541 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 3601 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 3661 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 3721 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 3781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 3841 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 3901 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 3961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 4021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 4081 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 4141 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 4201 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 4261 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 4321 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 4381 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 4441 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 4501 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 4561 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 4621 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 4681 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 4741 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 4801 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 4861 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 4921 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 4981 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 5041 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 5101 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 5161 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 5221 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 5281 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 5341 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 5401 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 5461 gcgcacattt ccccgaaaag tgccacctga cgtc //
pcDNA3.1/Myc-His A载体图谱 pcDNA3.1/Myc-HisA质粒图谱pdf版和pcDNA3.1/Myc-His A载体序列 pcDNA3.1/Myc-HisA质粒序列等相关资料下载
pcDNA3.1/Myc-His A质粒 pcDNA3.1/Myc-HisA载体应用举例
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