| 质粒名称: | pAAV-fNPY-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22912 |
| 存储实验室: | Edward Callaway |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4000 bp |
| 完整质粒大小: | 8300 bp |
| 载体类型: | AAV,哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | fugu neuropeptide Y promoter driving GFP |
| 物种来源: | takifugu rubripes (河豚) |
| 插入大小: | 4300 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | Unknown |
| 3' 测序引物 | SP6 |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV-fNPY-GFP was a gift from Edward Callaway (爱迪基因 plasmid # 22912) |
| 参考文献部分: | Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM. Front Neural Circuits. 2009 . 3():19. 10.3389/neuro.04.019.2009 PubMed 19949461 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAAV-fNPY-GFP 8248 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAAV-fNPY-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8248)
AUTHORS Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
Callaway EM
TITLE Short Promoters in Viral Vectors Drive Selective Expression in
Mammalian Inhibitory Neurons, but do not Restrict Activity to
Specific Inhibitory Cell-Types.
JOURNAL Front Neural Circuits. 2009 . 3():19.
PUBMED 19949461
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-fNPY-GFP|
FEATURES Location/Qualifiers
source 1..8248
/organism="synthetic DNA construct"
/mol_type="other DNA"
intron 2874..3349
/label=-beta-globin intron
/note="internally truncated intron from human beta-globin"
primer_bind 3431..3447
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3431..3447
/label=pBluescriptSK
/note="For pBluescript vector"
regulatory 3480..3489
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3486..4202
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3531..3552)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3792..3811)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4139..4160
/label=EGFP-C
/note="EGFP, forward primer"
promoter complement(4263..4281)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4264..4281)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4301..4318)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4307..4418
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 4562..4691
/label=AAV2 ITR
rep_origin 5062..5575
/direction=RIGHT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5149..5168)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5359..5380
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6057..6076)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6176..6198
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6236..6254)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6322..6426
/gene="bla"
/label=AmpR promoter
CDS 6427..7287
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6645..6664)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7458..8046
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7947..7966
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8200..8217
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 ctctccccgc gcgttggccg attcattaat gcagctgcgc gctcgctcgc tcactgaggc
61 cgcccgggca aagcccgggc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
121 aactccatca ctaggggttc cttgtagtta atgattaacc cgccatgcta cttatctacg
181 tagccatgct ctagaggatc cttcgaaaag cttgtatacg gccggccacg cgtatgaagg
241 gagggaaatt cagaaccaag cgaatacaat aagaaagaaa tacaaaagaa aagcaaattg
301 acttggtgct tgatttctga tgtttcaacg tattttcaca catgatagca tatggttgct
361 atggagaagg gatgcctcat tttgtccata attacagttt attttagttt ttttttttct
421 aattgactta agcaaccgga gctacattgg aggtttttgc tatcttatca ctgatattat
481 tagtgctgat gttaatcatt attaatggac atattaccct gactcccact tagcatcgcc
541 atttctctct cctcgagctg gaaaagatac aaaatcctcc tgtatgctca tagaaatcct
601 gccaaattct tccaaaatct gatctgcttg tcagagctat tttaattata gaaggtatgg
661 tgaatattta attaatcaag ctgattgaga tggtctaatc cgctgtttgc tcgtgtgaag
721 acagcagcaa atgaaggaaa gatgttgttt tattgccgat gttgatgcgt atccttcgac
781 actcgcgccg tttcccggct gacgtgcaga gggttcaggc tcggatcccc gtttcctgtg
841 tggactctcc ctcttctccc cgttcctgca catcccgctt cactgttctg ttcctgccct
901 gcccgtcgga gtgttgacgg tcatttgttc ctccatctgg gcccactgat ggattcgcct
961 ccacctccca gataccagat attcgctggt ttcactgcat cttcgtgtta ttaatgcgat
1021 gctgtttccc tccctggagc agatgtttga gatggcaaag aatattttcc catttcacac
1081 cccacccccc ctgtaaatgg agggctgaga ctaagaagcc attgtagcag ctcttcttgg
1141 tcatctccta ttttgagtgt gaaggtgatg gcgcctcacc ttttcctacc aaaggacagg
1201 atatcaccag gaagaaacag ctattttaga taaaaatgat gaatatttaa caccccgttt
1261 ggattttcat gttgtttttg cctttgtgcg atgacatcag tgcactttta agattttttt
1321 tcttcttcct tgtagtttaa aggccttttc aatcgtggta ggggtgttac atttaactta
1381 gaagtaatta cacacactcc ttgcctcctt tgacggctgc tgtgactcca aactcatgct
1441 cctgggcttg aacccgtctg ctccagctgc ttggatctca acagatcacc tgtttatcag
1501 cctggagctg tgaagagcgc actgcaaaca caggctgacc tctcacccct gcaatcaggc
1561 acattttaat ttcaagccct tcactccaac gagcccgtaa gacgtcgtca ttctgtgcat
1621 gtgtggactc ggttctggtt ccacgcacgt tctcactcgt catctctctt tgagcatcct
1681 gagttgtaca aaaaaagccc ctaacgcatc ctccaggagc agcacggctg gataatggtg
1741 atttttgacg ctccaagcat gcgtacaaaa ggccgatgaa tgggaagaag atgaagcact
1801 tagtgatgca gactcatctg aagccttatt tgggttttct tattaagggg gttgattgac
1861 aggcctccat attcatgttt agggcttttg ttcacattga agacttcgag tccctctttt
1921 ccatctgatg cagaagggat gggggggggg ggggggataa gcgatctaat tcatcacaca
1981 tcccctcaag ttagtgtgca cgcgagtctg gctgactctt ttcatttctc gcgcttcttt
2041 cagggaggaa gactcaagtt tggttgcagc tcttttgtga cttcagatgt gattattgat
2101 ctcattatgc agaagaaatg cgtcttgatt gtgaaacctc agagacaccg aagctcccac
2161 actcagcacg agtgtttatc tgcgttgtgt gtgtgtgtgt gcgtgtgtgt gtgtgtgtgt
2221 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtac acgcacctgc gtctgagttg
2281 ccctatcaaa attggtttaa tggtgtttcc aggtgcaaat cacagtgcac cgtttagctg
2341 ttgattaatt cccccctttt ttaacaccct aaattctctc tttagctgct ggaatgggca
2401 gatttgctcg tctccctcca tcctcggcct ctcgggttct tctttacgct gttctacgtg
2461 ttttatatta aaagaccccg tttaaaaggt gacagcatca ccagtgacgt ccacacggag
2521 aatgcagagg aactctgacg cgctgttcca cagccccttt gcgcgcacat ataaatccca
2581 ccgcgcagcc agccgccacc aaagtcgagg agacgccacg ggattacgca caaagccacc
2641 ctgaaccact gggaaacgta agtactttgc tttcaaatct gaaaaattaa aacttctcaa
2701 aaggtgcgac tttgttaatc tgtttgaagg gcgattaaag caactcaatt taacaaaatc
2761 gcagattatc cccgggtttt aaatatattg gggaaagaaa tgtatataaa atagatgtga
2821 ttttagatgg gtacgctagc ggcgcgccga gctcggatcc tgagaacttc agggtgagtc
2881 tatgggaccc ttgatgtttt ctttcccctt cttttctatg gttaagttca tgtcatagga
2941 aggggagaag taacagggta cacatattga ccaaatcagg gtaattttgc atttgtaatt
3001 ttaaaaaatg ctttcttctt ttaatatact tttttgttta tcttatttct aatactttcc
3061 ctaatctctt tctttcaggg caataatgat acaatgtatc atgcctcttt gcaccattct
3121 aaagaataac agtgataatt tctgggttaa ggcaatagca atatttctgc atataaatat
3181 ttctgcatat aaattgtaac tgatgtaaga ggtttcatat tgctaatagc agctacaatc
3241 cagctaccat tctgctttta ttttatggtt gggataaggc tggattattc tgagtccaag
3301 ctaggccctt ttgctaatca tgttcatacc tcttatcttc ctcccacagc tcctgggcaa
3361 cgtgctggtc tgtgtgctgg cccatcactt tggcaaagca cgtgaattcg aagatctggc
3421 cgcctcggcc tctagaacta gtggatcccc cgggctgcag gaattcgatg ctaccggtcg
3481 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
3541 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
3601 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
3661 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
3721 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
3781 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
3841 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
3901 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
3961 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
4021 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
4081 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
4141 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
4201 agtccggact cagatcctac tagtaggatc tcgagtttaa accgtacggg aaacggccct
4261 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
4321 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
4381 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
4441 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
4501 gcggccgctc tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac
4561 aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
4621 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
4681 cgagcgcgca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
4741 cgcagcctga atggcgaatg gcgattccgt tgcaatggct ggcggtaata ttgttctgga
4801 tattaccagc aaggccgata gtttgagttc ttctactcag gcaagtgatg ttattactaa
4861 tcaaagaagt attgcgacaa cggttaattt gcgtgatgga cagactcttt tactcggtgg
4921 cctcactgat tataaaaaca cttctcagga ttctggcgta ccgttcctgt ctaaaatccc
4981 tttaatcggc ctcctgttta gctcccgctc tgattctaac gaggaaagca cgttatacgt
5041 gctcgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
5101 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
5161 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
5221 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
5281 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
5341 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
5401 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
5461 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttaaa
5521 tatttgctta tacaatcttc ctgtttttgg ggcttttctg attatcaacc ggggtacata
5581 tgattgacat gctagtttta cgattaccgt tcatcgattc tcttgtttgc tccagactct
5641 caggcaatga cctgatagcc tttgtagaga cctctcaaaa atagctaccc tctccggcat
5701 gaatttatca gctagaacgg ttgaatatca tattgatggt gatttgactg tctccggcct
5761 ttctcacccg tttgaatctt tacctacaca ttactcaggc attgcattta aaatatatga
5821 gggttctaaa aatttttatc cttgcgttga aataaaggct tctcccgcaa aagtattaca
5881 gggtcataat gtttttggta caaccgattt agctttatgc tctgaggctt tattgcttaa
5941 ttttgctaat tctttgcctt gcctgtatga tttattggat gttggaatcg cctgatgcgg
6001 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
6061 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
6121 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
6181 agctgcatgt gtcagaggtt ttcaccatca tcaccgaaac gcgcgagacg aaagggcctc
6241 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
6301 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
6361 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
6421 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
6481 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
6541 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
6601 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
6661 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
6721 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
6781 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
6841 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
6901 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
6961 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
7021 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
7081 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
7141 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
7201 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
7261 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
7321 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
7381 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
7441 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
7501 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
7561 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
7621 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
7681 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
7741 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
7801 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
7861 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
7921 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
7981 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
8041 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
8101 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
8161 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
8221 gcggaagagc gcccaatacg caaaccgc
//
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