| 质粒名称: | BB3nK_ext_AD |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 108687 |
| 存储实验室: | Brigitte Gasser |
|
载体骨架基本信息 |
|
| 载体名称: | BB3nK_ext_AD |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 2642 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，酵母表达 |
| 筛选标记: | G418 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | BB3nK_ext_AD was a gift from Brigitte Gasser (Addgene plasmid # 108687 ; http://n2t.net/addgene:108687 ; RRID:Addgene_108687) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS BB3nK_ext_AD 2642 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Plasmid for assembly of donor DNA templates consisting of three
expression units.
ACCESSION .
VERSION .
KEYWORDS BB3nK_ext_AD
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2642)
AUTHORS Gassler T, Heistinger L, Mattanovich D, Gasser B, Prielhofer R.
TITLE CRISPR/Cas9-mediated homology directed genome editing in Pichia
pastoris
JOURNAL (2018) In Press
REFERENCE 2 (bases 1 to 2642)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2642
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(62..650)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(142..161)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
gene 785..2141
/label=kanMX
/note="yeast selectable marker conferring kanamycin
resistance (Wach et al., 1994)"
promoter 785..1128
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
CDS 1129..1938
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(1196..1215)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
terminator 1944..2141
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
ORIGIN
1 cgctgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
481 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
661 tgatcttttc tacggggtgg agcgcaaaaa accccgcccc tgacagggcg gggttttttc
721 gcgatcgaag acccagctgg agaccctcga gggatccggt ctccgatcgg gtcttcagct
781 catggacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc
841 atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc
901 catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacgtg
961 cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta
1021 gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa
1081 aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaggaa
1141 aagactcacg tttcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
1201 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
1261 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
1321 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
1381 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg caaaacagca
1441 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
1501 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
1561 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
1621 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg
1681 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
1741 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
1801 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
1861 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
1921 ctcgatgagt ttttctaatc agtactgaca ataaaaagat tcttgttttc aagaacttgt
1981 catttgtata gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt
2041 atattttttt tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa
2101 tatcatgcgt caatcgtatg tgaatgctgg tcgctatact ggcttgtaag caatctggac
2161 aattatgtaa gcgggttacg taaacagtta tgtaagcaga aaaatttcaa acgacaaaac
2221 ttggggtcta cagacacagt agccagaaga ttgcactacc attcgactcc tcatgaccca
2281 ctctttcgat ccatgtagtt aggttaccgt ttttcctaat atttaaggat gttgaaaatt
2341 cattttcatt tttttcgttt ttaagatttt ctcacaactc ttccaaagat tactagttga
2401 cttttcaaat atttagggta tttttctcac tttttcctag caaactccaa ttggtgggtt
2461 cagtgcaatg gagtatcacc ttgcaaccac aacgtaatag ctaacttgtg gccaccatgt
2521 ctggttgtag agataattgg attctaatgt ggatcacatg actactcacg tgtcaaaaac
2581 ccaacctgac ttggcccagc ttagcaagaa tatttcgaat ccactcttgt ggcctagtgg
2641 ac
//
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