质粒名称: | pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro,pX334 |
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出品公司: | 爱迪基因 |
目录编号: | 42333 |
存储实验室: | Feng Zhang |
载体骨架基本信息 |
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载体名称: | pUC ori vector |
载体抗性: | 氨苄霉素 |
载体类型: | 哺乳细胞表达 |
筛选标记: | 嘌呤霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | humanized S. pyogenes Cas9 (D10A) nickase |
插入大小: | 4272 bp |
基因突变: | D10A nickase-converting mutation |
标签/融合蛋白: | HA(N端) |
文献引用方法 |
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材料和方法部分: | pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro was a gift from Feng Zhang (Addgene plasmid # 42333) |
参考文献部分: | Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan 3. 10.1126/science.1231143 PubMed 23287718 |
pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-P
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro 10151 bp DNA SYN DEFINITION pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..10151 /organism="pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro" /mol_type="other DNA" promoter 10..242 /label="hU6_promoter" misc_feature 172..191 /label="LKO1_5_primer" misc_feature 394..663 /label="CAG_enhancer" CDS complement(1180..3780) /label="ORF frame 3" CDS 1201..5400 /label="ORF frame 1" misc_feature 1204..1230 /label="HA" misc_feature 1234..1254 /label="SV40 NLS" misc_feature 1240..1254 /label="NLS" misc_feature complement(5425..5442) /label="BGH_rev_primer" terminator 5428..5631 /label="bGH_PA_terminator" misc_feature 5787..5806 /label="H1_primer" misc_feature 6029..6050 /label="EGFP_C_primer" misc_feature complement(6126..6159) /label="loxP" misc_feature complement(6255..6278) /label="MSCV_rev_primer" CDS 6499..7356 /label="ORF frame 1" misc_feature 6650..6669 /label="mPGK_F_primer" CDS complement(6730..7407) /label="ORF frame 3" gene 6757..7356 /label="puro (variant)" /gene="puro (variant)" rep_origin 7646..7952 /label="f1_origin" misc_feature 8220..8242 /label="pGEX_3_primer" promoter 8401..8429 /label="AmpR_promoter" gene 8471..9331 /label="Ampicillin" /gene="Ampicillin" CDS 8471..9331 /label="ORF frame 2" rep_origin 9486..10105 /label="pBR322_origin" ORIGIN 1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA 241 CGAAACACCG GGTTTTAGAG CTATGCTGTT TTGAATGGTC CCAAAACggG TCTTCgaGAA 301 GACGTTTTAG AGCTATGCTG TTTTGAATGG TCCCAAAACT TTTTctagcg cgtgcgccaa 361 ttctgcagac aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct 421 ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc 481 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 541 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 601 tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 661 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc 721 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg 781 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg 841 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt 901 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc 961 gctgcgacgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg 1021 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg 1081 ctgtaattag ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat 1141 gtttaattac ctggagcacc tgcctgaaat cacttttttt caggttGGac cggtgccacc 1201 ATGTACCCAT ACGATGTTCC AGATTACGCT TCGCCGAAGA AAAAGCGCAA GGTCGAAGCG 1261 TCCGACAAGA AGTACAGCAT CGGCCTGGCC ATCGGCACCA ACTCTGTGGG CTGGGCCGTG 1321 ATCACCGACG AGTACAAGGT GCCCAGCAAG AAATTCAAGG TGCTGGGCAA CACCGACCGG 1381 CACAGCATCA AGAAGAACCT GATCGGAGCC CTGCTGTTCG ACAGCGGCGA AACAGCCGAG 1441 GCCACCCGGC TGAAGAGAAC CGCCAGAAGA AGATACACCA GACGGAAGAA CCGGATCTGC 1501 TATCTGCAAG AGATCTTCAG CAACGAGATG GCCAAGGTGG ACGACAGCTT CTTCCACAGA 1561 CTGGAAGAGT CCTTCCTGGT GGAAGAGGAT AAGAAGCACG AGCGGCACCC CATCTTCGGC 1621 AACATCGTGG ACGAGGTGGC CTACCACGAG AAGTACCCCA CCATCTACCA CCTGAGAAAG 1681 AAACTGGTGG ACAGCACCGA CAAGGCCGAC CTGCGGCTGA TCTATCTGGC CCTGGCCCAC 1741 ATGATCAAGT TCCGGGGCCA CTTCCTGATC GAGGGCGACC TGAACCCCGA CAACAGCGAC 1801 GTGGACAAGC TGTTCATCCA GCTGGTGCAG ACCTACAACC AGCTGTTCGA GGAAAACCCC 1861 ATCAACGCCA GCGGCGTGGA CGCCAAGGCC ATCCTGTCTG CCAGACTGAG CAAGAGCAGA 1921 CGGCTGGAAA ATCTGATCGC CCAGCTGCCC GGCGAGAAGA AGAATGGCCT GTTCGGCAAC 1981 CTGATTGCCC TGAGCCTGGG CCTGACCCCC AACTTCAAGA GCAACTTCGA CCTGGCCGAG 2041 GATGCCAAAC TGCAGCTGAG CAAGGACACC TACGACGACG ACCTGGACAA CCTGCTGGCC 2101 CAGATCGGCG ACCAGTACGC CGACCTGTTT CTGGCCGCCA AGAACCTGTC CGACGCCATC 2161 CTGCTGAGCG ACATCCTGAG AGTGAACACC GAGATCACCA AGGCCCCCCT GAGCGCCTCT 2221 ATGATCAAGA GATACGACGA GCACCACCAG GACCTGACCC TGCTGAAAGC TCTCGTGCGG 2281 CAGCAGCTGC CTGAGAAGTA CAAAGAGATT TTCTTCGACC AGAGCAAGAA CGGCTACGCC 2341 GGCTACATTG ACGGCGGAGC CAGCCAGGAA GAGTTCTACA AGTTCATCAA GCCCATCCTG 2401 GAAAAGATGG ACGGCACCGA GGAACTGCTC GTGAAGCTGA ACAGAGAGGA CCTGCTGCGG 2461 AAGCAGCGGA CCTTCGACAA CGGCAGCATC CCCCACCAGA TCCACCTGGG AGAGCTGCAC 2521 GCCATTCTGC GGCGGCAGGA AGATTTTTAC CCATTCCTGA AGGACAACCG GGAAAAGATC 2581 GAGAAGATCC TGACCTTCCG CATCCCCTAC TACGTGGGCC CTCTGGCCAG GGGAAACAGC 2641 AGATTCGCCT GGATGACCAG AAAGAGCGAG GAAACCATCA CCCCCTGGAA CTTCGAGGAA 2701 GTGGTGGACA AGGGCGCTTC CGCCCAGAGC TTCATCGAGC GGATGACCAA CTTCGATAAG 2761 AACCTGCCCA ACGAGAAGGT GCTGCCCAAG CACAGCCTGC TGTACGAGTA CTTCACCGTG 2821 TATAACGAGC TGACCAAAGT GAAATACGTG ACCGAGGGAA TGAGAAAGCC CGCCTTCCTG 2881 AGCGGCGAGC AGAAAAAGGC CATCGTGGAC CTGCTGTTCA AGACCAACCG GAAAGTGACC 2941 GTGAAGCAGC TGAAAGAGGA CTACTTCAAG AAAATCGAGT GCTTCGACTC CGTGGAAATC 3001 TCCGGCGTGG AAGATCGGTT CAACGCCTCC CTGGGCACAT ACCACGATCT GCTGAAAATT 3061 ATCAAGGACA AGGACTTCCT GGACAATGAG GAAAACGAGG ACATTCTGGA AGATATCGTG 3121 CTGACCCTGA CACTGTTTGA GGACAGAGAG ATGATCGAGG AACGGCTGAA AACCTATGCC 3181 CACCTGTTCG ACGACAAAGT GATGAAGCAG CTGAAGCGGC GGAGATACAC CGGCTGGGGC 3241 AGGCTGAGCC GGAAGCTGAT CAACGGCATC CGGGACAAGC AGTCCGGCAA GACAATCCTG 3301 GATTTCCTGA AGTCCGACGG CTTCGCCAAC AGAAACTTCA TGCAGCTGAT CCACGACGAC 3361 AGCCTGACCT TTAAAGAGGA CATCCAGAAA GCCCAGGTGT CCGGCCAGGG CGATAGCCTG 3421 CACGAGCACA TTGCCAATCT GGCCGGCAGC CCCGCCATTA AGAAGGGCAT CCTGCAGACA 3481 GTGAAGGTGG TGGACGAGCT CGTGAAAGTG ATGGGCCGGC ACAAGCCCGA GAACATCGTG 3541 ATCGAAATGG CCAGAGAGAA CCAGACCACC CAGAAGGGAC AGAAGAACAG CCGCGAGAGA 3601 ATGAAGCGGA TCGAAGAGGG CATCAAAGAG CTGGGCAGCC AGATCCTGAA AGAACACCCC 3661 GTGGAAAACA CCCAGCTGCA GAACGAGAAG CTGTACCTGT ACTACCTGCA GAATGGGCGG 3721 GATATGTACG TGGACCAGGA ACTGGACATC AACCGGCTGT CCGACTACGA TGTGGACCAT 3781 ATCGTGCCTC AGAGCTTTCT GAAGGACGAC TCCATCGACA ACAAGGTGCT GACCAGAAGC 3841 GACAAGAACC GGGGCAAGAG CGACAACGTG CCCTCCGAAG AGGTCGTGAA GAAGATGAAG 3901 AACTACTGGC GGCAGCTGCT GAACGCCAAG CTGATTACCC AGAGAAAGTT CGACAATCTG 3961 ACCAAGGCCG AGAGAGGCGG CCTGAGCGAA CTGGATAAGG CCGGCTTCAT CAAGAGACAG 4021 CTGGTGGAAA CCCGGCAGAT CACAAAGCAC GTGGCACAGA TCCTGGACTC CCGGATGAAC 4081 ACTAAGTACG ACGAGAATGA CAAGCTGATC CGGGAAGTGA AAGTGATCAC CCTGAAGTCC 4141 AAGCTGGTGT CCGATTTCCG GAAGGATTTC CAGTTTTACA AAGTGCGCGA GATCAACAAC 4201 TACCACCACG CCCACGACGC CTACCTGAAC GCCGTCGTGG GAACCGCCCT GATCAAAAAG 4261 TACCCTAAGC TGGAAAGCGA GTTCGTGTAC GGCGACTACA AGGTGTACGA CGTGCGGAAG 4321 ATGATCGCCA AGAGCGAGCA GGAAATCGGC AAGGCTACCG CCAAGTACTT CTTCTACAGC 4381 AACATCATGA ACTTTTTCAA GACCGAGATT ACCCTGGCCA ACGGCGAGAT CCGGAAGCGG 4441 CCTCTGATCG AGACAAACGG CGAAACCGGG GAGATCGTGT GGGATAAGGG CCGGGATTTT 4501 GCCACCGTGC GGAAAGTGCT GAGCATGCCC CAAGTGAATA TCGTGAAAAA GACCGAGGTG 4561 CAGACAGGCG GCTTCAGCAA AGAGTCTATC CTGCCCAAGA GGAACAGCGA TAAGCTGATC 4621 GCCAGAAAGA AGGACTGGGA CCCTAAGAAG TACGGCGGCT TCGACAGCCC CACCGTGGCC 4681 TATTCTGTGC TGGTGGTGGC CAAAGTGGAA AAGGGCAAGT CCAAGAAACT GAAGAGTGTG 4741 AAAGAGCTGC TGGGGATCAC CATCATGGAA AGAAGCAGCT TCGAGAAGAA TCCCATCGAC 4801 TTTCTGGAAG CCAAGGGCTA CAAAGAAGTG AAAAAGGACC TGATCATCAA GCTGCCTAAG 4861 TACTCCCTGT TCGAGCTGGA AAACGGCCGG AAGAGAATGC TGGCCTCTGC CGGCGAACTG 4921 CAGAAGGGAA ACGAACTGGC CCTGCCCTCC AAATATGTGA ACTTCCTGTA CCTGGCCAGC 4981 CACTATGAGA AGCTGAAGGG CTCCCCCGAG GATAATGAGC AGAAACAGCT GTTTGTGGAA 5041 CAGCACAAGC ACTACCTGGA CGAGATCATC GAGCAGATCA GCGAGTTCTC CAAGAGAGTG 5101 ATCCTGGCCG ACGCTAATCT GGACAAAGTG CTGTCCGCCT ACAACAAGCA CCGGGATAAG 5161 CCCATCAGAG AGCAGGCCGA GAATATCATC CACCTGTTTA CCCTGACCAA TCTGGGAGCC 5221 CCTGCCGCCT TCAAGTACTT TGACACCACC ATCGACCGGA AGAGGTACAC CAGCACCAAA 5281 GAGGTGCTGG ACGCCACCCT GATCCACCAG AGCATCACCG GCCTGTACGA GACACGGATC 5341 GACCTGTCTC AGCTGGGAGG CGACAGCCCC AAGAAGAAGA GAAAGGTGGA GGCCAGCtaa 5401 gaattcCTAG AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG 5461 TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT 5521 AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG 5581 GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGAgAA TAGCAGGCAT GCTGGGGAgc 5641 ggccgcAATT CGAACGCTGA CGTCATCAAC CCGCTCCAAG GAATCGCGGG CCCAGTGTCA 5701 CTAGGCGGGA ACACCCAGCG CGCGTGCGCC CTGGCAGGAA GATGGCTGTG AGGGACAGGG 5761 GAGTGGCGCC CTGCAATATT TGCATGTCGC TATGTGTTCT GGGAAATCAC CATAAACGTG 5821 AAATGTCTTT GGATTTGGGA ATCTTATAAG TTCTGTATGA GACCACAGAT CTAgttggaa 5881 ccattcaaaa cagcatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg 5941 gcaccgagtc ggtgcttttt ggcgccgaca accactacct gagcacccag tccgccctga 6001 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 6061 ggatcactct cggcatggac gagctgtaca agtaaGAATT Ccgatcatat tcaataaccc 6121 ttaatataac ttcgtataat gtatgctata cgaagttatt aggtctgaag aggagtttac 6181 gtccagccaa gcttaggatc tcgacctcga aattctaccg ggtaggggag gcgcttttcc 6241 caaggcagtc tggagcatgc gctttagcag ccccgctggc acttggcgct acacaagtgg 6301 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg 6361 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca 6421 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat 6481 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct 6541 ttgctccttc gctttctggg ctcagcagct gggaagggtg ggtccggggg cgggctcagg 6601 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg 6661 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc 6721 tgcatccatc tagatctcga gcagctgaag cttaccatga ccgagtacaa gcccacggtg 6781 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 6841 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 6901 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 6961 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 7021 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 7081 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 7141 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 7201 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 7261 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 7321 cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc 7381 ccgaccgaaa ggagcgcacg accccatgca tcgatgatat cagatccccg ggatgcagaa 7441 attgatgatc tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact 7501 ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg gagcggcgcc 7561 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca 7621 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 7681 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 7741 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 7801 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg 7861 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 7921 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt 7981 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 8041 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag 8101 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga 8161 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 8221 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg 8281 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 8341 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 8401 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 8461 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 8521 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 8581 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 8641 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 8701 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 8761 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 8821 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 8881 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 8941 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 9001 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 9061 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 9121 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 9181 gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 9241 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 9301 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 9361 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 9421 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 9481 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 9541 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 9601 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 9661 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 9721 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 9781 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 9841 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 9901 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 9961 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 10021 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 10081 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 10141 tgctcacatg t //