| 质粒名称: | p415-GalL-Cas9-CYC1t |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 43804 |
| 存储实验室: |
George Church
|
|
载体骨架基本信息 |
|
| 载体名称: | p415 |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 6670 bp |
| 完整质粒大小: | 10810 bp |
| 载体类型: | 酵母表达，CRISPR |
| 筛选标记: | LEU2 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Human Optimized S. pyogenes Cas9 |
| 物种来源: | H. sapiens (human), S. cerevisiae (budding yeast); S.pyogenes |
| 插入大小: | 4140 bp |
| 启动子 | GalL |
| 克隆方法: | 未知 |
| 5' 测序引物 | T3 promoter |
| 3' 测序引物 | T7 promoter |
|
文献引用方法 |
|
| 材料和方法部分: | p415-GalL-Cas9-CYC1t was a gift from George Church (爱迪基因 plasmid # 43804) |
| 参考文献部分: | Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.. Nucleic Acids Res 10.1093/nar/gkt135 PubMed 23460208 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS p415-GalL-Cas9-CYC1t 10793 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS p415-GalL-Cas9-CYC1t
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10793)
AUTHORS Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
TITLE Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas
systems.
JOURNAL Nucleic Acids Res
PUBMED 23460208
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|p415-GalL-Cas9-CYC1t|
FEATURES Location/Qualifiers
source 1..10793
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(10..28)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
misc_feature 70..573
/label=CEN/ARS
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
promoter 610..714
/gene="bla"
/label=AmpR promoter
CDS 715..1575
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(933..952)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1746..2334
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2235..2254
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2488..2505
/label=L4440
/note="L4440 vector, forward primer"
promoter 2658..2688
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2696..2712
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2701..2723
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2720..2736
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2720..2736
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 2755..2775
/label=T3
/note="T3 promoter, forward primer"
promoter 2757..2775
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind 3184..3207
/label=GAL1
/note="S. cerevisiae GAL1 promoter, forward primer"
primer_bind 3228..3244
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3228..3244
/label=pBluescriptSK
/note="For pBluescript vector"
CDS 3258..7361
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 7374..7394
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
terminator 7410..7657
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(7426..7444)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
primer_bind complement(7675..7694)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(7676..7694)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(7704..7721)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(7704..7720)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7713..7735)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 7865..8320
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7952..7971)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8162..8183
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 8620..9024
/gene="S. cerevisiae LEU2"
/label=LEU2 promoter
CDS 9037..10131
/codon_start=1
/gene="S. cerevisiae LEU2"
/product="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/label=LEU2
/note="yeast auxotrophic marker"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA"
primer_bind complement(10624..10643)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 10743..10765
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
2761 aaccctcact aaagggaaca aaagctggag ctcgtgacag ccctccgaag gaagactctc
2821 ctccgtgcgt cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc
2881 actgctccga acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat
2941 tggcagtaac ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat
3001 gataatgcga ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt
3061 tttgatctat taacggatat ataaatgcaa aaactgcata accactttaa ctaatacttt
3121 caacattttc ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt
3181 gttaatatac ctctatactt taacgtcaag gagaaaaaac cccggattct agaactagtg
3241 gatcccccgg gaaaaaaatg gacaagaagt actccattgg gctcgatatc ggcacaaaca
3301 gcgtcggttg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc
3361 tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc ctgttcgact
3421 ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga tatacccgca
3481 gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct aaggtggatg
3541 actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa aagcacgagc
3601 gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag tacccaacca
3661 tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg cggttgatct
3721 atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag ggggacctga
3781 acccagacaa cagcgatgtc gacaaactct ttatccaact ggttcagact tacaatcagc
3841 ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc ctgagcgcta
3901 ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga
3961 acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccccaac tttaaatcta
4021 acttcgacct ggccgaagat gccaagcttc aactgagcaa agacacctac gatgatgatc
4081 tcgacaatct gctggcccag atcggcgacc agtacgcaga cctttttttg gcggcaaaga
4141 acctgtcaga cgccattctg ctgagtgata ttctgcgagt gaacacggag atcaccaaag
4201 ctccgctgag cgctagtatg atcaagcgct atgatgagca ccaccaagac ttgactttgc
4261 tgaaggccct tgtcagacag caactgcctg agaagtacaa ggaaattttc ttcgatcagt
4321 ctaaaaatgg ctacgccgga tacattgacg gcggagcaag ccaggaggaa ttttacaaat
4381 ttattaagcc catcttggaa aaaatggacg gcaccgagga gctgctggta aagcttaaca
4441 gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc caccagattc
4501 acctgggcga actgcacgct atcctcaggc ggcaagagga tttctacccc tttttgaaag
4561 ataacaggga aaagattgag aaaatcctca catttcggat accctactat gtaggccccc
4621 tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa atcagaagag accatcactc
4681 cctggaactt cgaggaagtc gtggataagg gggcctctgc ccagtccttc atcgaaagga
4741 tgactaactt tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt
4801 acgagtactt cacagtttat aacgagctca ccaaggtcaa atacgtcaca gaagggatga
4861 gaaagccagc attcctgtct ggagagcaga agaaagctat cgtggacctc ctcttcaaga
4921 cgaaccggaa agttaccgtg aaacagctca aagaagacta tttcaaaaag attgaatgtt
4981 tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg ggaacgtatc
5041 acgatctcct gaaaatcatt aaagacaagg acttcctgga caatgaggag aacgaggaca
5101 ttcttgagga cattgtcctc acccttacgt tgtttgaaga tagggagatg attgaagaac
5161 gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat gaaacagctc aagaggcgcc
5221 gatatacagg atgggggcgg ctgtcaagaa aactgatcaa tgggatccga gacaagcaga
5281 gtggaaagac aatcctggat tttcttaagt ccgatggatt tgccaaccgg aacttcatgc
5341 agttgatcca tgatgactct ctcaccttta aggaggacat ccagaaagca caagtttctg
5401 gccaggggga cagtcttcac gagcacatcg ctaatcttgc aggtagccca gctatcaaaa
5461 agggaatact gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata
5521 agcccgagaa tatcgttatc gagatggccc gagagaacca aactacccag aagggacaga
5581 agaacagtag ggaaaggatg aagaggattg aagagggtat aaaagaactg gggtcccaaa
5641 tccttaagga acacccagtt gaaaacaccc agcttcagaa tgagaagctc tacctgtact
5701 acctgcagaa cggcagggac atgtacgtgg atcaggaact ggacatcaat cggctctccg
5761 actacgacgt ggatcatatc gtgccccagt cttttctcaa agatgattct attgataata
5821 aagtgttgac aagatccgat aaaaatagag ggaagagtga taacgtcccc tcagaagaag
5881 ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa cgccaaactg atcacacaac
5941 ggaagttcga taatctgact aaggctgaac gaggtggcct gtctgagttg gataaagccg
6001 gcttcatcaa aaggcagctt gttgagacac gccagatcac caagcacgtg gcccaaattc
6061 tcgattcacg catgaacacc aagtacgatg aaaatgacaa actgattcga gaggtgaaag
6121 ttattactct gaagtctaag ctggtctcag atttcagaaa ggactttcag ttttataagg
6181 tgagagagat caacaattac caccatgcgc atgatgccta cctgaatgca gtggtaggca
6241 ctgcacttat caaaaaatat cccaagcttg aatctgaatt tgtttacgga gactataaag
6301 tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga aataggcaag gccaccgcta
6361 agtacttctt ttacagcaat attatgaatt ttttcaagac cgagattaca ctggccaatg
6421 gagagattcg gaagcgacca cttatcgaaa caaacggaga aacaggagaa atcgtgtggg
6481 acaagggtag ggatttcgcg acagtccgga aggtcctgtc catgccgcag gtgaacatcg
6541 ttaaaaagac cgaagtacag accggaggct tctccaagga aagtatcctc ccgaaaagga
6601 acagcgacaa gctgatcgca cgcaaaaaag attgggaccc caagaaatac ggcggattcg
6661 attctcctac agtcgcttac agtgtactgg ttgtggccaa agtggagaaa gggaagtcta
6721 aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat catggagcga tcaagcttcg
6781 aaaaaaaccc catcgacttt ctcgaggcga aaggatataa agaggtcaaa aaagacctca
6841 tcattaagct tcccaagtac tctctctttg agcttgaaaa cggccggaaa cgaatgctcg
6901 ctagtgcggg cgagctgcag aaaggtaacg agctggcact gccctctaaa tacgttaatt
6961 tcttgtatct ggccagccac tatgaaaagc tcaaagggtc tcccgaagat aatgagcaga
7021 agcagctgtt cgtggaacaa cacaaacact accttgatga gatcatcgag caaataagcg
7081 aattctccaa aagagtgatc ctcgccgacg ctaacctcga taaggtgctt tctgcttaca
7141 ataagcacag ggataagccc atcagggagc aggcagaaaa cattatccac ttgtttactc
7201 tgaccaactt gggcgcgcct gcagccttca agtacttcga caccaccata gacagaaagc
7261 ggtacacctc tacaaaggag gtcctggacg ccacactgat tcatcagtca attacggggc
7321 tctatgaaac aagaatcgac ctctctcagc tcggtggaga cagcagggct gaccccaaga
7381 agaagaggaa ggtgtgatct cttctcgagt catgtaatta gttatgtcac gcttacattc
7441 acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta
7501 ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat
7561 ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct
7621 tgagaaggtt ttgggacgct cgaaggcttt aatttgcggc cggtacccaa ttcgccctat
7681 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
7741 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
7801 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
7861 cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
7921 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
7981 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
8041 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
8101 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
8161 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
8221 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
8281 tttaacgcga attttaacaa aatattaacg tttacaattt cctgatgcgg tattttctcc
8341 ttacgcatct gtgcggtatt tcacaccgca tatcgacggt cgaggagaac ttctagtata
8401 tccacatacc taatattatt gccttattaa aaatggaatc ccaacaatta catcaaaatc
8461 cacattctct tcaaaatcaa ttgtcctgta cttccttgtt catgtgtgtt caaaaacgtt
8521 atatttatag gataattata ctctatttct caacaagtaa ttggttgttt ggccgagcgg
8581 tctaaggcgc ctgattcaag aaatatcttg accgcagtta actgtgggaa tactcaggta
8641 tcgtaagatg caagagttcg aatctcttag caaccattat ttttttcctc aacataacga
8701 gaacacacag gggcgctatc gcacagaatc aaattcgatg actggaaatt ttttgttaat
8761 ttcagaggtc gcctgacgca tatacctttt tcaactgaaa aattgggaga aaaaggaaag
8821 gtgagaggcc ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg
8881 catcacaata cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg
8941 gttagcaatc gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat
9001 atatttcaag gatataccat tctaatgtct gcccctatgt ctgcccctaa gaagatcgtc
9061 gttttgccag gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa
9121 gctatttctg atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt
9181 gctgctatcg atgctacagg tgtcccactt ccagatgagg cgctggaagc ctccaagaag
9241 gttgatgccg ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga
9301 cctgaacaag gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca
9361 tgtaactttg catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa
9421 ggtactgact tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag
9481 gaagacgatg gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa
9541 agaatcacaa gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg
9601 tccttggata aagctaatgt tttggcctct tcaagattat ggagaaaaac tgtggaggaa
9661 accatcaaga acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc
9721 atgatcctag ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt
9781 ggtgatatca tctccgatga agcctccgtt atcccaggtt ccttgggttt gttgccatct
9841 gcgtccttgg cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac
9901 ggttctgctc cagatttgcc aaagaataag gtcaacccta tcgccactat cttgtctgct
9961 gcaatgatgt tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca
10021 gttaaaaagg ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc
10081 accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct
10141 ctttttttat gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg
10201 aaattacaaa atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca
10261 tttatcaaga aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa ttgatactaa
10321 tggctcaacg tgataaggaa aaagaattgc actttaacat taatattgac aaggaggagg
10381 gcaccacaca aaaagttagg tgtaacagaa aatcatgaaa ctatgattcc taatttatat
10441 attggaggat tttctctaaa aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg
10501 accatttgat ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt
10561 ccctcaagaa ttttactctg tcagaaacgg ccttacgacg tagtcgatat ggtgcactct
10621 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
10681 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
10741 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cga
//
