| 质粒名称: | pLPC myc hPOT1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 12387 |
| 存储实验室: | Titia de Lange |
|
载体骨架基本信息 |
|
| 载体名称: | pLPC |
| 载体出品公司: | -- |
| 空载体大小: | 5700 bp |
| 完整质粒大小: | 7582 bp |
| 载体修饰: | -- |
| 载体类型: | 逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Protection of Telomeres 1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1900 bp |
| 基因突变: | -- |
| GenBank编号 | FLJ11073 |
| 启动子: | -- |
| 标签/融合蛋白: | Myc（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI(未破坏) |
| 3’克隆位点: | XhoI(未破坏) |
| 5’测序引物 | TAGGCGTGTACGGTGGGA |
| 3’测序引物 | CTTGCCAAACCTACAGGT |
|
文献引用方法 |
|
| 材料和方法部分: | pLPC myc hPOT1 was a gift from Titia de Lange (Addgene plasmid # 12387 ; http://n2t.net/addgene:12387 ; RRID:Addgene_12387) |
| 参考文献部分: | POT1 as a terminal transducer of TRF1 telomere length control. Loayza D, De Lange T. Nature. 2003 Jun 26. 423(6943):1013-8. 10.1038/nature01688 PubMed 12768206 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLPC myc hPOT1 7582 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLPC myc hPOT1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7582)
AUTHORS Loayza D, De Lange T
TITLE POT1 as a terminal transducer of TRF1 telomere length control.
JOURNAL Nature. 2003 Jun 26. 423(6943):1013-8.
PUBMED 12768206
REFERENCE 2 (bases 1 to 7582)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7582
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 120..589
/note="long terminal repeat from Moloney murine leukemia
virus"
CDS complement(2694..2723)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
regulatory 2723..2732
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind complement(2763..2782)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2764..2782)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(2827..3030)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(2856..2876)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 3031..3410
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 3582..3601
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
CDS complement(3690..4289)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3773..3793)
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 4270..4289
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
CDS complement(4307..4723)
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind complement(4323..4339)
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(4355..4377)
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
misc_feature 4924..5123
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
LTR 5188..5657
/note="long terminal repeat from Moloney murine leukemia
virus"
promoter 5688..5792
/gene="bla"
/label=AmpR promoter
CDS 5793..6653
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6011..6030)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6813..7401
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7302..7321
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7555..7572
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
721 ttctggagac taaataaaat cttttatttt atcggccctc tagatgcatg ctcgagttag
781 attacatctt ctgcaactgt ggtgtcaaaa atctgatagc aaatttgatt atctgttcca
841 tttgtgacat tgtatgactt gatgaagcat tccaaccacg gatatgcatc aatttttatt
901 cctggaggac aaaacatatc catgatcata tccacacttt tctgaaggtc atcatccatc
961 agaacttctg atgctggaat ctggaagaat ttgtcagaat ccatgagata ggcttctagt
1021 actcctgttc catcatcaag tgtaaaggtc ataacaaaca catattggag gggtacaata
1081 cccagtgctt ctgccacaga agaaggaatc cacgatgttt tatcaaccag ggaatttaga
1141 ttttgtatgg atctcaaact agaacactgt ttacatccat agtgatgtat tgttccttgt
1201 ataagaaatg gtgctgaaag gtccaaaagt tccaggtctt cgtggccaga tctcacagga
1261 attacactat taaacttgtt cgagagtttg caaatttcac tgagtgtacc tccttctatc
1321 aaaagtagac attcatttga aagcgggaga ataccattat ttttcacaaa atgaactgct
1381 acttttcgtc ctttttgatt tttagtggtc cagatttttg aatcatataa tgatgtattt
1441 tgtagcttga catctggggt tttagttgca ccatcctgaa aaattatatc caaatcgccc
1501 tcatgtggaa cttcttgcag caaatgacat ttagggcaat gaagtttaac agactgaaat
1561 agtcttctgg gcttatatga cctcaatttt gctcggatgc ggtattgttg aggagctttt
1621 tgtttcaaaa tggcacatag tggtgtcctc tccaaatact gatgatctgt aagtattgta
1681 gcagatagct gttgacatct ttctacctcg tataatgata ctgatccaga gcttggaaag
1741 ctgtcgtcag gttctgattg acagataaca tctgaatgct gattggctgt caaatttgca
1801 gattctaaat cctttttcag ttgatccaca tcagagttac tttctggcaa gaccctgatt
1861 ccccgaccgt aactggtacc tccatgaaga tgaaactcta aacttaacat tgtctgattc
1921 tctgaattca ttgattgaag tttggtatga aggctataga ttctaagaaa gcttccaacc
1981 ttcagagatc ttgccacatg aacatggtta tcgtagacta aaatgtctat tgtcagattt
2041 tgtagccgat ggatgtgact taaatcacct tcaagaacaa ggtcttgtat taagactctc
2101 caagatggaa atggtgtcct ggtgccatcc cataccttta gaagaaatga tgctccgtcc
2161 acttctgctt tgcccaagag ctgacaagtc aggtcaaaat actgcattgg ctgaacatca
2221 cacaatttta gtaatgtcca agacggtgac atatgagtag atgcccaaac acgtaaggct
2281 tctaccattt tgtggtcctc agtagtgaag ttaaaatact tgcttgaagt gcgaggtatg
2341 ataggggctc ccaaagttcc ctcaaacgtc aaagatgcaa agccagagct ggtgataccc
2401 tgagtctcct ttttatatac ttgaatcttc agcctgtgaa agcgaacaat atctccattt
2461 ttataaatta ttggaagggc ttcatagttt ccactaaaga gcaggcaagt tagttttaca
2521 tttgtctggt ccacaatagt tacaactgag caataatcag ttcctttgct tagatatggg
2581 ggcttaaaga acttcacaac accatagaca ttgacaattg taccaccctt aagttgattc
2641 aggggtgtat atatataatt tgttgctgga accaaggatc ctctctttct taacaaatct
2701 tcttcagaaa tcaacttttg ttccatggtg gctgatccga gctcggtacc aagcttgggt
2761 ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag
2821 ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
2881 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
2941 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
3001 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
3061 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
3121 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
3181 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
3241 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
3301 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
3361 taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc aacgcgtata
3421 tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc ctaagcagat tcttcatgca
3481 attgtcggtc aagccttgcc ttgttgtagc ttaaattttg ctcgcgcact actcagcgac
3541 ctccaacaca caagcaggga gcagatactg gcttaactat gcggcatcag agcagattgt
3601 actgagagtg caccataggg gatcgggaga tccgatgcat ggggtcgtgc gctcctttcg
3661 gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag
3721 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
3781 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
3841 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
3901 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
3961 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
4021 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
4081 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
4141 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
4201 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg
4261 aggcgcaccg tgggcttgta ctcggtcatg gtaagctgat ccggccggcg cctagagaag
4321 gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt tcaaggggga
4381 gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag ggcttgaccc
4441 agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc tggtctgggt
4501 gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg tctcggttaa
4561 aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag cagaaggtaa
4621 cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg acatctttcc
4681 agtgacctaa ggtcaaactt aagggagtgg taacagtctg gccctaattt tcagacaaat
4741 acagaaacac agtcagacag agacaacaca gaacgatgct gcagcgctgc agcagacaag
4801 acgcgcggct tcggttccaa accgaaagca aaaattcaga cggaggcggg aactgtttta
4861 ggttctcgtc tcctaccaga accacatatc ctgacggggt cggattccac atcgactccc
4921 ttcctcaggt cgggccacaa aaacggcccc caaagtccct gggacgtctc ccagggttgc
4981 ggccgggtgt tcagaactcg tcagttccac cacgggtccg ccagatacag agctagttag
5041 ctaactagta ccgacgcagg cgcataaaat cagtcataga cactagacaa tcggacagac
5101 acagataagt tgctggccag cttgcctccc ggtggtgggt cggtggtccc tgggcagggg
5161 tctcccgatc ccggacgagc ccccaaatga aagacccccg ctgacgggta gtcaatcact
5221 cagaggagac cctcccaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt
5281 tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag
5341 gggttgtggg ctcttttatt gagctcgggg agcagaagcg cgcgaacaga agcgagaagc
5401 gaactgattg gttagttcaa ataaggcaca gggtcatttc aggtccttgg ggcaccctgg
5461 aaacatctga tggttctcta gaaactgctg agggctggac cgcatctggg gaccatctgt
5521 tcttggccct gagccggggc aggaactgct taccacagat atcctgtttg gcccatattc
5581 agctgttcca tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct
5641 gtttggccca tattcaggct gcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
5701 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
5761 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
5821 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
5881 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
5941 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
6001 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
6061 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
6121 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
6181 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
6241 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
6301 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
6361 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
6421 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
6481 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
6541 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
6601 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
6661 accaagttta ctcatatata ctttagattg atttgcggcc gcaaaaggat ctaggtgaag
6721 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
6781 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
6841 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
6901 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
6961 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
7021 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
7081 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
7141 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
7201 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
7261 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
7321 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
7381 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
7441 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
7501 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
7561 tcagtgagcg aggaagcgga ag
//
