³öÆ·¹«Ë¾: | Invitrogen |
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ÔØÌåÃû³Æ: | pENTR-GUS |
ÖÊÁ£ÀàÐÍ: | GatewayÔØÌ壻ÈëÃÅÔØÌå |
¸ß¿½±´/µÍ¿½±´: | ¸ß¿½±´ |
Æô¶¯×Ó: | ÎÞ |
¿Ë¡·½·¨: | ÏÞÖÆÐÔÄÚÇÐø£»¶à¿Ë¡λµã |
ÔØÌå´óС: | 3841 bp |
5' ²âÐòÒýÎï¼°ÐòÁÐ: | -- |
3' ²âÐòÒýÎï¼°ÐòÁÐ: | -- |
ÔØÌå±êÇ©: | ÎÞ |
ÔØÌ忹ÐÔ: | ¿¨ÄÇùËØ |
ÕæºËɸѡ±ê¼Ç: | ÎÞ |
¿Ë¡¾úÖê: | DH5a ¡¢TOP10¸ÐÊÜ̬ϸ°û |
±¸×¢: | -- |
²úƷĿ¼ºÅ: | -- |
²¡¶¾/·Ç²¡¶¾: | ·Ç²¡¶¾ |
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LOCUS Exported 3841 bp ds-DNA circular SYN 13-AUG-2016 DEFINITION synthetic circular DNA KEYWORDS pENTR-GUS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3841) AUTHORS Biofeng Lab. FEATURES Location/Qualifiers source 1..3841 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 99..198 /gene="mutant version of attL" /bound_moiety="LR Clonase(TM)" /note="attL1" /note="recombination site for the Gateway(R) LR reaction" CDS 228..2039 /codon_start=1 /gene="uidA (or gusA) from E. coli" /product="beta-glucuronidase" /note="GUS" /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK PKSAAFLLQKRWTGMNFGEKPQQGGKQ" protein_bind complement(2041..2140) /gene="mutant version of attL" /bound_moiety="LR Clonase(TM)" /note="attL2" /note="recombination site for the Gateway(R) LR reaction" rep_origin complement(2259..2847) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2990..3805) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" ORIGIN 1 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga 61 cacgggccag agctgcagct agcatggatc tcgggcccca aataatgatt ttattttgac 121 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt 181 tgtacaaaaa agcaggctca tttaacttta agaaggagat atataccatg gtccgtcctg 241 tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc agtctggatc 301 gcgaaaactg tggaattgat cagcgttggt gggaaagcgc gttacaagaa agccgggcaa 361 ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc agatattcgt aattatgcgg 421 gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg 481 tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg ggtcaataat caggaagtga 541 tggagcatca gggcggctat acgccatttg aagccgatgt cacgccgtat gttattgccg 601 ggaaaagtgt acgtatcacc gtttgtgtga acaacgaact gaactggcag actatcccgc 661 cgggaatggt gattaccgac gaaaacggca agaaaaagca gtcttacttc catgatttct 721 ttaactatgc cggaatccat cgcagcgtaa tgctctacac cacgccgaac acctgggtgg 781 acgatatcac cgtggtgacg catgtcgcgc aagactgtaa ccacgcgtct gttgactggc 841 aggtggtggc caatggtgat gtcagcgttg aactgcgtga tgcggatcaa caggtggttg 901 caactggaca aggcactagc gggactttgc aagtggtgaa tccgcacctc tggcaaccgg 961 gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag ccagacagag tgtgatatct 1021 acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc 1081 acaaaccgtt ctactttact ggctttggtc gtcatgaaga tgcggactta cgtggcaaag 1141 gattcgataa cgtgctgatg gtgcacgacc acgcattaat ggactggatt ggggccaact 1201 cctaccgtac ctcgcattac ccttacgctg aagagatgct cgactgggca gatgaacatg 1261 gcatcgtggt gattgatgaa actgctgctg tcggctttaa cctctcttta ggcattggtt 1321 tcgaagcggg caacaagccg aaagaactgt acagcgaaga ggcagtcaac ggggaaactc 1381 agcaagcgca cttacaggcg attaaagagc tgatagcgcg tgacaaaaac cacccaagcg 1441 tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt 1501 tcgcgccact ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca 1561 atgtaatgtt ctgcgacgct cacaccgata ccatcagcga tctctttgat gtgctgtgcc 1621 tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt ggaaacggca gagaaggtac 1681 tggaaaaaga acttctggcc tggcaggaga aactgcatca gccgattatc atcaccgaat 1741 acggcgtgga tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt 1801 atcagtgtgc atggctggat atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg 1861 gtgaacaggt atggaatttc gccgattttg cgacctcgca aggcatattg cgcgttggcg 1921 gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc 1981 aaaaacgctg gactggcatg aacttcggtg aaaaaccgca gcagggaggc aaacaataat 2041 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca 2101 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct agcggtaata 2161 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 2221 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 2281 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 2341 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 2401 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 2461 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 2521 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 2581 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 2641 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 2701 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 2761 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 2821 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 2881 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagcttgcg ccgtcccgtc 2941 aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact 3001 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt 3061 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa 3121 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc 3181 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg 3241 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct 3301 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga 3361 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc 3421 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata 3481 cctggaatgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac 3541 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca 3601 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg 3661 catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca ttatcgcgag 3721 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgagcaag 3781 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca 3841 g //
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