出品公司: | -- |
---|---|
载体名称: | pLenti6-tdTomato-V5-DEST |
质粒类型: | 慢病毒载体;gateway载体;荧光报告载体 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | gateway |
启动子: | CMV |
载体大小: | 10113 bp |
5' 测序引物及序列: | CMV fwd :CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素、氯霉素 |
筛选标记: | blasticidin |
克隆菌株: | 阳性筛选用DB3.1 ,阴性筛选用OmniMAX2-T1, 重组慢病毒载体的复制用Stbl3感受态细胞,以维持质粒的稳定性。 |
宿主细胞(系): | 哺乳动物细胞 |
备注: |
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产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 慢病毒 |
买家导航
pLenti6-tdTomato-V5-DEST载体质粒基本信息
pLenti6-tdTomato-V5-DEST质粒图谱载体图谱和pLenti6-tdTomato-V5-DEST载体序列质粒序列多克隆位点信息
pLenti6-tdTomato-V5-DEST质粒载体简介
pLenti6-tdTomato-V5-DEST质粒序列载体序列
LOCUS Exported 10113 bp ds-DNA circular SYN 11-AUG-2016 KEYWORDS pLenti6-tdTomato-V5-DEST FEATURES Location/Qualifiers source 1..10113 /organism="Lentiviral vector pLenti6-tdTomato-V5-Blast" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University, Belgium) accession number LMBP 8401." /db_xref="taxon:1783462" misc_feature 1..16 /note="cPPT" /note="HIV-1 cPPT" regulatory 17..251 /regulatory_class="terminator" /note="(dU3)3' LTR" /note="RSV/HIV-1 dU3/3' LTR" regulatory 323..397 /regulatory_class="terminator" /note="SV40 polyA" /note="SV40 polyA early" regulatory complement(323..397) /regulatory_class="terminator" /note="SV40 polyA late" rep_origin 533..608 /note="SV40 ori" regulatory complement(642..658) /regulatory_class="promoter" /note="T7" /note="T7 promoter" rep_origin 826..1281 /note="f1 ori" /note="f1 origin" gene 1412..2272 /gene="bla" /note="Amp" CDS 1412..2272 /codon_start=1 /transl_table=11 /gene="bla" /product="beta-lactamase" /note="bla" /note="ampicillin resistance" /db_xref="GI:982281016" /protein_id="CVH74126.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 2813..3215 /note="pMB1" /note="pMB1 ori" regulatory 3307..3390 /regulatory_class="promoter" /note="lac promoter" regulatory 3454..3470 /regulatory_class="promoter" /note="T3 promoter" regulatory 3579..3907 /regulatory_class="promoter" /note="5' LTR" /note="RSV/HIV-1 5' LTR" misc_feature 4018..4062 /note="psi" /note="HIV-1 psi packaging signal-psi" misc_feature 4572..4805 /note="RRE" /note="HIV-1 Rev response element" regulatory 5304..5820 /regulatory_class="promoter" /note="CMV promoter" /note="hCMV-IE promoter and enhancer" regulatory 5937..6061 /regulatory_class="attenuator" /note="attR1" /note="gateway recombination site attR1" gene 6170..6829 /gene="cat" /note="cat" CDS 6170..6829 /codon_start=1 /transl_table=11 /gene="cat" /product="chloramphenicol acetyl transferase" /note="CAT" /note="chloramphenicol resistance" /db_xref="GI:982281017" /protein_id="CVH74127.1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" gene 7171..7476 /gene="ccdB" /note="ccdB" CDS 7171..7476 /codon_start=1 /transl_table=11 /gene="ccdB" /product="ccdB" /note="ccdB" /note="B gene of the control of cell death locus of the Escherichia coli F plasmid" /db_xref="GI:982281018" /protein_id="CVH74128.1" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" regulatory 7517..7641 /regulatory_class="attenuator" /note="attR2" /note="gateway recombination site attR2" gene 7673..9172 /gene="tdTomato" /note="tdTomato" CDS 7673..9172 /codon_start=1 /transl_table=11 /gene="tdTomato" /product="tdTomato" /note="tdTomato" /note="Orange-red fluorescent protein tandem dimer with V5 epitope tag" /db_xref="GI:982281019" /protein_id="CVH74130.1" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYKEGPRFEGKPIPNPLLGLDSTRTG" regulatory 9193..9475 /regulatory_class="promoter" /note="SV40 promoter" /note="SV40 early promoter" rep_origin 9432..9507 /note="SV40 ori" regulatory 9580..9645 /regulatory_class="promoter" /note="EM7" /note="synthetic EM7 promoter" gene 9646..10044 /gene="bsr" /note="bsr" CDS 9646..10044 /codon_start=1 /transl_table=11 /gene="bsr" /product="blasticidin S-resistance" /note="blasticidin" /db_xref="GI:982281020" /protein_id="CVH74131.1" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" ORIGIN 1 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt 61 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 121 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 181 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 241 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 301 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 361 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 421 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg 481 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 541 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 601 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg 661 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 721 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 781 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg 841 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 901 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 961 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 1021 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 1081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 1141 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 1201 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 1261 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat 1321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 1381 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 1441 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 1501 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 1561 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 1621 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 1681 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 1741 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 1801 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 1861 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 1921 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 1981 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 2041 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 2101 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 2161 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 2221 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 2281 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 2341 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 2401 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 2461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 2521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 2581 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 2641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 2701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 2761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 2821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 2881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 2941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 3001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 3061 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 3121 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 3181 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 3241 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 3301 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 3361 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 3421 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 3481 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta 3541 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg 3601 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt 3661 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac 3721 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 3781 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 3841 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 3901 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc 3961 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga 4021 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca 4081 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa 4141 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag 4201 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac 4261 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct 4321 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg 4381 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga 4441 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt 4501 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga 4561 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc 4621 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag 4681 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg 4741 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag 4801 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat 4861 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg 4921 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac 4981 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat 5041 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc 5101 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga 5161 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa 5221 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga 5281 tctcgacggt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg 5341 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 5401 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 5461 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 5521 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 5581 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 5641 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 5701 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 5761 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 5821 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga 5881 agacaccgac tctagaggat ccactagtcc agtgtggtgg aattctgcag atatcaacaa 5941 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt 6001 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat 6061 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg 6121 gattttgagt taggatccgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa 6181 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc 6241 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt 6301 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc 6361 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat 6421 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc 6481 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt 6541 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt 6601 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga 6661 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct 6721 gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat 6781 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa gatctggatc 6841 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag 6901 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt 6961 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata 7021 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct 7081 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct 7141 cttttgctga cgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga 7201 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga 7261 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt 7321 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt 7381 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc 7441 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccgt tatacacagc 7501 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg 7561 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc 7621 agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtatggtgag 7681 caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat 7741 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca 7801 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc 7861 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta 7921 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg 7981 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt 8041 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg 8101 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca 8161 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat 8221 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac 8281 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca 8341 cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca ccgcctcctc 8401 cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg 8461 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 8521 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat 8581 cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc 8641 cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 8701 ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta 8761 caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac 8821 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga 8881 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat 8941 ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga 9001 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg 9061 ccaccacctg ttcctgtacg gcatggacga gctgtacaag gagggcccgc ggttcgaagg 9121 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgagt 9181 ttggaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 9241 ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 9301 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 9361 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 9421 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 9481 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 9541 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca 9601 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg 9661 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc 9721 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt 9781 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct 9841 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc 9901 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa 9961 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc 10021 tctggttatg tgtgggaggg ctaagcacaa ttcgagctcg gtacctttaa gaccaatgac 10081 ttacaaggca gctgtagatc ttagccactt ttt //
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pLenti6-tdTomato-V5-DEST质粒载体应用举例
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