| 质粒名称: | pTNT-B18R |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 58978 |
| 存储实验室: | Steven Dowdy |
|
载体骨架基本信息 |
|
| 载体名称: | pTNT |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2871 bp |
| 完整质粒大小: | 3907 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | B18R,ankyrin repeat protein |
| 物种来源: | Vaccinia Virus |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | T7/Sp6 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | MfeI（克隆期间销毁） |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | SP6 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pTNT-B18R was a gift from Steven Dowdy (Addgene plasmid # 58978 ; http://n2t.net/addgene:58978 ; RRID:Addgene_58978) |
| 参考文献部分: | Efficient generation of human iPSCs by a synthetic self-replicative RNA. Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001. 10.1016/j.stem.2013.06.001 PubMed 23910086 |
买家导航
pTNTB18R载体基本信息
pTNTB18R载体图谱质粒图谱和多克隆位点信息
pTNTB18R载体简介
pTNTB18R载体序列
LOCUS pTNT-B18R 3907 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION expresses B18R protein from Western vaccinia virus that binds to and
neutralizes type I IFNs.
ACCESSION .
VERSION .
KEYWORDS pTNT-B18R
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3907)
AUTHORS Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR,
Chi NC, Fu XD, Yu BD, Dowdy SF
TITLE Efficient generation of human iPSCs by a synthetic self-replicative
RNA.
JOURNAL Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi:
10.1016/j.stem.2013.06.001.
PUBMED 23910086
REFERENCE 2 (bases 1 to 3907)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3907
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 16..34
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 40..58
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 40..57
/label=SP6
/note="SP6 promoter, forward primer"
regulatory 94..103
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
terminator 1197..1244
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 1323..1778
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1410..1429)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1620..1641
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(1845..1864)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1964..1986
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(2024..2042)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 2110..2214
/gene="bla"
/label=AmpR promoter
CDS 2215..3075
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2433..2452)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3246..3834
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3735..3754
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 taaggctaga gtacttaata cgactcacta taggctagca tttaggtgac actatagaat
61 acaagctact tgttcttttt gcactcgaga attgccacca tgggcacgat gaaaatgatg
121 gtacatatat atttcgtatc attattgtta ttgctattcc acagttacgc catagacatc
181 gaaaatgaaa tcacagaatt cttcaataaa atgagagata ctctaccagc taaagactct
241 aaatggttga atccagcatg tatgttcgga ggcacaatga atgatatagc cgctctagga
301 gagccattca gcgcaaagtg tcctcctatt gaagacagtc ttttatcgca cagatataaa
361 gactatgtgg ttaaatggga aaggctagaa aaaaatagac ggcgacaggt ttctaataaa
421 cgtgttaaac atggtgattt atggatagcc aactatacat ctaaattcag taaccgtagg
481 tatttgtgca ccgtaactac aaagaatggt gactgtgttc agggtatagt tagatctcat
541 attagaaaac ctccttcatg cattccaaaa acatatgaac taggtactca tgataagtat
601 ggcatagact tatactgtgg aattctttac gcaaaacatt ataataatat aacttggtat
661 aaagataata aggaaattaa tatcgacgac attaagtatt cacaaacggg aaaggaatta
721 attattcata atccagagtt agaagatagc ggaagatacg actgttacgt tcattacgac
781 gacgttagaa tcaagaatga tatcgtagta tcaagatgta aaatacttac ggttataccg
841 tcacaagacc acaggtttaa actaatacta gatccaaaaa tcaacgtaac gataggagaa
901 cctgccaata taacatgcac tgctgtgtca acgtcattat tgattgacga tgtactgatt
961 gaatgggaaa atccatccgg atggcttata ggattcgatt ttgatgtata ctctgtttta
1021 actagtagag gcggtattac cgaggcgacc ttgtactttg aaaatgttac tgaagaatat
1081 ataggtaata catataaatg tcgtggacac aactattatt ttgaaaaaac ccttacaact
1141 acagtagtat tggagtaagc ggccgcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaactag
1201 cataacccct tggggcctct aaacgggtct tgaggggttt tttggatccg ggctggcgta
1261 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
1321 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
1381 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
1441 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
1501 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
1561 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
1621 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
1681 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
1741 taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt
1801 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
1861 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
1921 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
1981 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
2041 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
2101 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
2161 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
2221 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
2281 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
2341 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
2401 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
2461 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
2521 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
2581 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
2641 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
2701 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
2761 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
2821 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
2881 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
2941 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
3001 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
3061 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
3121 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
3181 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
3241 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
3301 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
3361 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
3421 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
3481 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
3541 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
3601 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
3661 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
3721 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
3781 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
3841 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga
3901 cagatct
//
