| 质粒名称: | pEBTet_NAD-Snifit-nucl |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 113915 |
| 存储实验室: | Kai Johnsson |
|
载体骨架基本信息 |
|
| 载体名称: | pEBTet |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 11448 bp |
| 完整质粒大小: | 13899 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | NAD-Snifit,hSPR(D41W42)-HaloTag7-Pro30-SNAPtag |
| 物种来源: | Synthetic |
| 插入大小: | 2349 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV TetO2 |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV_Fwd |
| 3' 测序引物 | BGH_Rev |
|
文献引用方法 |
|
| 材料和方法部分: | pEBTet_NAD-Snifit-nucl was a gift from Kai Johnsson (Addgene plasmid # 113915 ; http://n2t.net/addgene:113915 ; RRID:Addgene_113915) |
| 参考文献部分: | Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K. Elife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638. 32638 [pii] PubMed 29809136 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEBTet_NAD-Snifit-nucl 13899 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Plasmid encoding the nuclear localization of the FRET semi-synthetic
biosensor measuring NAD+ concentration in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pEBTet_NAD-Snifit-nucl
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13899)
AUTHORS Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K
TITLE Semisynthetic biosensors for mapping cellular concentrations of
nicotinamide adenine dinucleotides.
JOURNAL Elife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.
PUBMED 29809136
REFERENCE 2 (bases 1 to 13899)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13899
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 807..1691
/codon_start=1
/product="modified bacterial dehalogenase that forms
covalent bonds with chloroalkane derivatives"
/label=HaloTag(R)
/translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
SEIARWLSTLEISG"
CDS 2373..2393
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2397..2417
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(2524..2541)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2530..2641
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3182..4971
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
primer_bind complement(7635..7653)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7721..7825
/gene="bla"
/label=AmpR promoter
CDS 7826..8686
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8044..8063)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8857..9445
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9346..9365
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(9634..9654)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9652..9949
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind 9881..9900
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 10026..10625
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(10026..10045)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 10522..10542
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 10716..10850
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10988..11007)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 11284..11545
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
intron 11662..12234
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind 12242..12261
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
primer_bind 12289..12308
/label=T7
/note="T7 promoter, forward primer"
promoter 12289..12307
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 12318..12935
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
primer_bind complement(12391..12410)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
polyA_signal 12980..13101
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
enhancer 13156..13535
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 13536..13739
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 13690..13710
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 13741..13759
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 13762..13780
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 13790..13814
/label=LNCX
/note="Human CMV promoter, forward primer"
ORIGIN
1 agcttaccac catggagggc gggctggggc gtgctgtgtg cttgctgacc ggggcctccc
61 gcggcttcgg ccggacgctg gccccgctcc tggcctcgct gctgtcgccc ggctccgtgc
121 ttgtccttag cgactggaac gacgaggcac tgcgccagct ggaggccgag ctgggcgccg
181 agcggtctgg cctgcgcgtg gtgcgggtgc ccgccgacct gggcgccgag gccggcttgc
241 agcagctgct cggcgccctg cgcgagctcc cccggcccaa ggggctgcag cgactgctgc
301 ttatcaacaa cgcgggctct cttggggatg tgtccaaagg cttcgtggac ctgagtgact
361 ccactcaagt gaacaactac tgggcactga acttgacctc catgctctgc ctgacttcca
421 gcgtcctgaa ggccttcccg gacagtcctg gcctcaacag aaccgtggtt aacatctcgt
481 ccctctgtgc cctgcaacct ttcaaaggct gggcgctgta ctgtgcagga aaggctgctc
541 gtgatatgct gttccaggtc ctggcgctgg aggaacctaa tgtgagggtg ctgaactatg
601 ccccaggtcc tctggacaca gacatgcagc agttggcccg ggagacctcc gtggacccag
661 acatgcgaaa agggctgcag gagctgaagg caaaggggaa gctggtggat tgcaaggtgt
721 cagcccagaa actgctgagc ttactggaaa aggacgagtt caagtctgga gcccacgtgg
781 acttctatga caaagaattc ggatccgaaa tcggtactgg ctttccattc gacccccatt
841 atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc
901 ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac atcatcccgc
961 atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc aaatccgaca
1021 aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc ttcatcgaag
1081 ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct ctgggtttcc
1141 actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag ttcatccgcc
1201 ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag gccttccgca
1261 ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc
1321 cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc gagccgttcc
1381 tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca atcgccggtg
1441 agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac cagtcccctg
1501 tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc gaagccgctc
1561 gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt ctgaatctgc
1621 tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg tctactctgg
1681 agatttccgg tagcggccgc cctcctcctc cgccaccgcc accaccgccg ccgccgccac
1741 caccacctcc tccgcctcct ccaccaccac cgccaccgcc tccaccacct ggcggccgct
1801 ctcggtctct cgagatggat aaagactgcg aaatgaaacg cactaccctg gacagcccgc
1861 tgggtaaact ggaactgagc ggttgcgagc agggcctgca cgaaatcatt ttcctgggca
1921 aaggtacgag cgctgcggat gcggtcgaag taccggctcc ggctgcggtt ctgggtggcc
1981 cggagccgct gatgcaggct accgcatggc tgaacgcata ttttcaccag ccagaggcga
2041 ttgaagagtt ccctgtaccg gccctgcatc acccggtatt tcagcaggaa tcctttaccc
2101 gtcaggtact gtggaaactg ctgaaagtag tgaaattcgg cgaggtgatc tcctattccc
2161 atctggcggc gctggctggc aacccagcgg caactgcggc ggtgaaaacc gctctgtctg
2221 gtaatccggt gccaattctg atcccgtgcc accgtgtggt tcagggcgac ctggacgtag
2281 gtggctacga aggcggcctg gctgtgaaag aatggctgct ggcgcacgaa ggtcaccgtc
2341 tgggtaaacc aggcctgggc ggagctccgg atccaaaaaa gaagagaaag gtagatccaa
2401 aaaagaagag aaaggtagat ccaaaaaaga agagaaagga gctccgggct tctcctcaat
2461 aatgaagcta gcggcgcgcc cggccgctcg agtctagagg gcccgtttaa acccgctgat
2521 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
2581 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
2641 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
2701 gggaggattg ggaagacaat agcaggctag aggatcgatc cccgccccgg acgaactaaa
2761 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
2821 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
2881 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
2941 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
3001 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
3061 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
3121 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
3181 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
3241 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
3301 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
3361 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
3421 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
3481 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
3541 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
3601 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
3661 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
3721 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
3781 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
3841 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
3901 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
3961 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
4021 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
4081 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
4141 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
4201 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
4261 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
4321 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
4381 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
4441 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
4501 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
4561 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
4621 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
4681 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
4741 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
4801 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
4861 atgcatatac agtcagcata tgatacccag tagtagagtg ggagtgctat cctttgcata
4921 tgccgccacc tcccaagggg gcgtgaattt tcgctgcttg tccttttcct gctggttgct
4981 cccattctta ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac
5041 caggtaaatg tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac
5101 gtgacaacat gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca
5161 gatggccaga aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc
5221 gggggaatac acggctttta atacgattga gggcgtctcc taacaagtta catcactcct
5281 gcccttcctc accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct
5341 ccgcggcagc cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa
5401 agctgcacac agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct
5461 taatcgcatc cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac
5521 agacaatgga ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg
5581 ggagacgact caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta
5641 ggttgtaaag ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt
5701 cgtcggtagt cctttctacg tgactcctag ccaggagagc tcttaaacct tctgcaatgt
5761 tctcaaattt cgggttggaa cctccttgac cacgatgctt ttccaaacca ccctcctttt
5821 ttgcgccctg cctccatcac cctgaccccg gggtccagtg cttgggcctt ctcctgggtc
5881 atctgcgggg ccctgctcta tcgctcccgg gggcacgtca ggctcaccat ctgggccacc
5941 ttcttggtgg tattcaaaat aatcggcttc ccctacaggg tggaaaaatg gccttctacc
6001 tggagggggc ctgcgcggtg gagacccgga tgatgatgac tgactactgg gactcctggg
6061 cctcttttct ccacgtccac gacctctccc cctggctctt tcacgacttc cccccctggc
6121 tctttcacgt cctctacccc ggcggcctcc actacctcct cgaccccggc ctccactacc
6181 tcctcgaccc cggcctccac tgcctcctcg accccggcct ccacctcctg ctcctgcccc
6241 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc
6301 tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctcc
6361 tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc
6421 ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgctcc
6481 tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc
6541 tgctcctgcc cctcctgctc ctgcccctcc tgcccctcct gcccctcctg ctcctgcccc
6601 tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
6661 ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc
6721 tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc
6781 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc
6841 ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc
6901 tgctcctgcc cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc
6961 aatgcaactt ggacgttttt ggggtctccg gacaccatct ctatgtcttg gccctgatcc
7021 tgagccgccc ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg
7081 tccatggtta tcaccccctc ttctttgagg tccactgccg ccggagcctt ctggtccaga
7141 tgtgtctccc ttctctccta ggccatttcc aggtcctgta cctggcccct cgtcagacat
7201 gattcacact aaaagagatc aatagacatc tttattagac gacgctcagt gaatacaggg
7261 agtgcagact cctgccccct ccaacagccc ccccaccctc atccccttca tggtcgctgt
7321 cagacagatc caggtctgaa aattccccat cctccgaacc atcctcgtcc tcatcaccaa
7381 ttactcgcag cccggaaaac tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa
7441 gccaggcctc aaattcctcg tccccctttt tgctggacgg tagggatggg gattctcggg
7501 acccctcctc ttcctcttca aggtcaccag acagagatgc tactggggca acggaagaaa
7561 agctgggtgc ggcctgtgag gatcagctta tcgatgataa gctgtcaaac atgagaattc
7621 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
7681 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
7741 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
7801 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
7861 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
7921 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
7981 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
8041 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
8101 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
8161 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
8221 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
8281 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
8341 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
8401 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
8461 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
8521 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
8581 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
8641 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
8701 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
8761 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
8821 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
8881 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
8941 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
9001 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
9061 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
9121 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
9181 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
9241 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
9301 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
9361 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
9421 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
9481 cttttgctgg ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc
9541 ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat
9601 ccagcctcgc gtcgaactag atgatccggc tgtggaatgt gtgtcagtta gggtgtggaa
9661 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
9721 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
9781 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
9841 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
9901 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga gggtgaccgc
9961 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac
10021 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
10081 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
10141 ccgaccgcca catcgaacgc gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
10201 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
10261 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
10321 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
10381 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
10441 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
10501 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
10561 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
10621 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga cccggtccga
10681 cgcggcccga cgggtccgag gggggtcgac ctcgaaactt gtttattgca gcttataatg
10741 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
10801 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatccgaac
10861 cccttcctcg accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt
10921 tgccattgct cgaccaattc tcatgtttga cagcttatca tcgcagatcc gtatggtgca
10981 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
11041 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
11101 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
11161 gggccagata tacgcgtatc tgaggggact agggtgtgtt taggcgaaaa gcggggcttc
11221 ggttgtacgc ggttaggagt cccctcagga tatagtagtt tcgcttttgc atagggaggg
11281 ggaaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
11341 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
11401 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
11461 attccgcatt gcagagatat tgtatttaag tgcctagctc gatacaataa acgccatttg
11521 accattcacc acattggtgt gcacctccaa gctggccggg gatcctctag ggcctctgag
11581 ctattccaga agtagtgaag aggctttttt ggaggcctag gcttttgcaa aaagctccgg
11641 atcgatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct
11701 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga
11761 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc
11821 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac
11881 tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag
11941 tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca
12001 cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat
12061 tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt catcatcctg
12121 cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag
12181 tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct acagctcctg
12241 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgta atacgactca
12301 ctatagggcg aattgatatg tctagattag ataaaagtaa agtgattaac agcgcattag
12361 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
12421 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
12481 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
12541 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
12601 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
12661 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
12721 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
12781 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
12841 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
12901 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc gtacagcgga tcccgggaat
12961 tcagatctta ttaaagcaga acttgtttat tgcagcttat aatggttaca aataaagcaa
13021 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
13081 caaactcatc aatgtatctt atcatgtctg gtcgaatcca tggcgcagaa ctggtaggta
13141 tggaagatcc gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
13201 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
13261 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
13321 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
13381 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
13441 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
13501 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
13561 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
13621 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
13681 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
13741 tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag
13801 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
13861 gggaccgatc cagcctccgg actctagcgt ttaaactta
//
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