| 出品公司: | Evrogen |
|---|---|
| 载体名称: | pKillerRed-B |
| 质粒类型: | 细菌质粒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | CMV |
| 载体大小: | 4128 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pKillerRedB载体基本信息
pKillerRedB载体图谱质粒图谱和多克隆位点信息
pKillerRedB载体简介
pKillerRedB载体序列
LOCUS pKillerRed-B 4128 bp ds-DNA circular SYN 04-JAN-2013
DEFINITION Vector for expressing KillerRed in bacteria.
ACCESSION .
VERSION .
KEYWORDS pKillerRed-B
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4128)
AUTHORS Evrogen
TITLE Direct Submission
JOURNAL Exported Friday, March 6, 2020 from SnapGene Viewer 3.1.4
http://www.biofeng.com/
FEATURES Location/Qualifiers
source 1..4128
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 115..117
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 133..852
/codon_start=1
/product="red fluorescent protein KillerRed, a
genetically-encoded photosensitizer"
/note="KillerRed"
/note="mammalian codon-optimized"
/translation="MGSEGGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFN
VHAVCETGKLPMSWKPICHLIQYGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDG
TMTSHHTYELDDTCVVSRITVNCDGFQPDGPIMRDQLVDILPNETHMFPHGPNAVRQLA
FIGFTTADGGLMMGHFDSKMTFNGSRAIEIPGPHFVTIITKQMRDTSDKRDHVCQREVA
YAHSVPRITSAIGSDED"
terminator 874..968
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 1012..1671
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1736..1822
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(2304..2892)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3063..3923)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
LIKHW"
promoter complement(3924..4028)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttgattaaag aggagaaatt aactatgaga
121 ggatcgggat ccatgggttc agagggcggc cccgccctgt tccagagcga catgaccttc
181 aaaatcttca tcgacggcga ggtgaacggc cagaagttca ccatcgtggc cgacggcagc
241 agcaagttcc cccacggcga cttcaacgtg cacgccgtgt gcgagaccgg caagctgccc
301 atgagctgga agcccatctg ccacctgatc cagtacggcg agcccttctt cgcccgctac
361 cccgacggca tcagccattt cgcccaggag tgcttccccg agggcctgag catcgaccgc
421 accgtgcgct tcgagaacga cggcaccatg accagccacc acacctacga gctggacgac
481 acctgcgtgg tgagccgcat caccgtgaac tgcgacggct tccagcccga cggccccatc
541 atgcgcgacc agctggtgga catcctgccc aacgagaccc acatgttccc ccacggcccc
601 aacgccgtgc gccagctggc cttcatcggc ttcaccaccg ccgacggcgg cctgatgatg
661 ggccacttcg acagcaagat gaccttcaac ggcagccgcg ccatcgagat ccccggccca
721 cacttcgtga ccatcatcac caagcagatg agggacacca gcgacaagcg cgaccacgtg
781 tgccagcgcg aggtggccta cgcccacagc gtgccccgca tcaccagcgc catcggtagc
841 gacgaggatt gaaagcttaa ttagctgagc ttggactcct gttgatagat ccagtaatga
901 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
961 gtgagaatcc aagctagctt ggcgagattt tcaggagcta aggaagctaa aatggagaaa
1021 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
1081 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
1141 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
1201 gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg
1261 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
1321 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
1381 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
1441 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
1501 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
1561 ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga
1621 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa
1681 ggcagttatt ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat
1741 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
1801 cgctctcctg agtaggacaa atccgccgct ctagagctgc ctcgcgcgtt tcggtgatga
1861 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
1921 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
1981 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
2041 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
2101 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
2161 tgtcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
2221 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
2281 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
2341 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
2401 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
2461 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
2521 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
2581 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
2641 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
2701 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
2761 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
2821 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
2881 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
2941 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
3001 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
3061 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
3121 atagctgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
3181 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
3241 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
3301 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
3361 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
3421 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
3481 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
3541 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
3601 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
3661 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
3721 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
3781 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
3841 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
3901 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
3961 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
4021 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
4081 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcac
//
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