| 出品公司: | -- |
|---|---|
| 载体名称: | pUC57-Tac-mCherry |
| 质粒类型: | 大肠杆菌载体；蛋白表达质粒 |
| 高拷贝/低拷贝: | -- |
| 复制子: | pUC |
| 启动子: | Tac |
| 载体大小: | 3740 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 大肠杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pUC57-Tac-mCherry 3740 bp ds-DNA circular SYN 04-七月-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pUC57-Tac-mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3740)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2016年9月18日 from SnapGene Viewer 2.8.1
http://www.biofeng.com/
FEATURES Location/Qualifiers
source 1..3740
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 379..395
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(435..512)
/gene="lacI"
/note="lacI promoter"
misc_feature 558..586
/note="tac promoter"
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
CDS 633..1343
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/note="mCherry"
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
terminator 1377..1424
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind complement(1519..1535)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1543..1559
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1567..1597)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 1612..1633
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1921..2509)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2680..3540)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3541..3645)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
421 tgcatctaga tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata
481 ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga
541 ctcctgcatt aggaaatttg acaattaatc atcggctcgt ataatgtgtg gaatgtctca
601 tgagcggata tttcacacag gaaacagtat tcatggtgag caagggcgag gaggataaca
661 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
721 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
781 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
841 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
901 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
961 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
1021 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
1081 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
1141 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
1201 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
1261 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
1321 gcatggacga gctgtacaag taattaacct aggctgctgc caccgctgag caataactag
1381 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa cctcaggcat
1441 ttgagaagca cacggtcaca catcggatcc cgggcccgtc gactgcagag gcctgcatgc
1501 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
1561 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
1621 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
1681 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
1741 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
1801 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
1861 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
1921 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
1981 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
2041 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
2101 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
2161 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
2221 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
2281 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
2341 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
2401 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
2461 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
2521 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
2581 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
2641 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
2701 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
2761 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
2821 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
2881 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
2941 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
3001 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
3061 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
3121 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
3181 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
3241 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
3301 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
3361 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
3421 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
3481 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
3541 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
3601 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
3661 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
3721 tatcacgagg ccctttcgtc
//
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