| 出品公司: | -- |
|---|---|
| 载体名称: | pTSU2-APP 载体 |
| 质粒类型: | 膜蛋白酵母双杂交系统 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | TEF1 |
| 载体大小: | 9897 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Kanamycin卡那 |
| 筛选标记: | LEU2 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTSU2-APP 3323 bp DNA circular SYN 13-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3323)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 13, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3323
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 948..968
/codon_start=1
/product="6xHis affinity tag"
/label=7xHis
/translation="HHHHHHH"
CDS 2430..3035
/codon_start=1
/gene="lexA"
/product="E. coli DNA-binding protein that represses genes
involved in the SOS response to DNA damage"
/label=LexA
/note="The N-terminal DNA-binding domain recognizes the
16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
/translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKGLKKQGN
KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
CDS 3054..3287
/codon_start=1
/gene="UL48"
/product="transcriptional activation domain of herpes
simplex virus protein VP16 (Triezenberg et al., 1988;
Cousens et al., 1989)"
/label=VP16 AD
/translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
1 atttctcagt ttcagtttca tttttcttgt tctattacaa ctttttttac ttcttgctca
61 ttagaaagaa agcatagcaa tctaatctaa gttttctaga tatatgatgc ttttgcaagc
121 tttccttttc cttttggctg gttttgcagc caaaatatct gcaatggcca ttacggccct
181 ggaggtaccc actgatggta atgctggcct gctggctgaa ccccagattg ccatgttctg
241 tggcagactg aacatgcaca tgaatgtcca gaatgggaag tgggattcag atccatcagg
301 gaccaaaacc tgcattgata ccaaggaagg catcctgcag tattgccaag aagtctaccc
361 tgaactgcag atcaccaatg tggtagaagc caaccaacca gtgaccatcc agaactggtg
421 caagcggggc cgcaagcagt gcaagaccca tccccacttt gtgattccct accgctgctt
481 agttggtgag tttgtaagtg atgcccttct cgttcctgac aagtgcaaat tcttacacca
541 ggagaggatg gatgtttgcg aaactcatct tcactggcac accgtcgcca aagagacatg
601 cagtgagaag agtaccaact tgcatgacta cggcatgttg ctgccctgcg gaattgacaa
661 gttccgaggg gtagaggttt gtgtgttgcc cactggctga agaaagtgac aatgtggatt
721 ctgctgatgc ggaggaggag gactcggatg tctggggggg gcggagcaga cacagactat
781 gcagatggga gtgaagacaa agtagtagaa gtagcagagg aggaagaagt ggctgaggtg
841 gaagaagaag aagccgatga tgacgaggac gatgaggatg gtgatgaggt agaggaagag
901 gctgaggaac cctacgaaga agccacagag agaaccacca gcattgccac caccaccacc
961 accaccacag agtctgtgga agaggtggtt cgagttccta caacagcagc cagtacccct
1021 gatgccgttg acaagtatct cgagacacct ggggatgaga atgaacatgc ccatttccag
1081 aaagccaaag agaggcttga ggccaagcac cgagagagaa tgtcccaggt catgagagaa
1141 tgggaagagg cagaacgtca agcaaagaac ttgcctaaag ctgataagaa ggcagttatc
1201 cagcatttcc aggagaaagt ggaatctttg gaacaggaag cagccaacga gagacagcag
1261 ctggtggaga cacacatggc cagagtggaa gccatgctca atgaccgccg ccgcctggcc
1321 ctggagaact acatcaccgc tctgcaggcg gttcctcctc ggcctcgtca cgtgttcaat
1381 atgctaaaga agtatgtccg cgcagaacag aaggacagac agcacaccct aaagcatttc
1441 gagcatgtgc gcatggtgga tcccaagaaa gccgctcaga tccggtccca ggttatgaca
1501 cacctccgtg tgatttatga gcgcatgaat cagtctctct ccctgctcta caacgtgcct
1561 gcagtggccg aggagattca ggatgaagtt gatgagctgc ttcagaaaga gcaaaactat
1621 tcagatgacg tcttggccaa catgattagt gaaccaagga tcagttacgg aaacgatgct
1681 ctcatgccat ctttgaccga aacgaaaacc accgtggagc tccttcccgt gaatggagag
1741 ttcagcctgg acgatctcca gcgtggcatt cttttggggc tgactctgtg ccagccaaca
1801 cagaaaacga agttgagcct gttgatgccc gccctgctgc cgaccgagga ctgaccactc
1861 gaccaggttc tgggttgaca aatatcaaga cggaggagat ctctgaagtg aagatggatg
1921 cagaattccg acatgactca ggatatgaag ttcatcatca aaaattggtg ttctttgcag
1981 aagatgtggg ttcaaacaaa ggtgcaatca ttggactcat ggtgggcggt gttgtcatag
2041 cgacagtgat cgtcatcacc ttggtgatgc tgaagaagaa acagtacaca tccattcatc
2101 atggtgtggt ggaggttgac gccgctgtca ccccagagga gcgccacctg tccaagatgc
2161 agcagaacgg ctacgaaaat ccaacctaca agttctttga gcagatgcag aacccggccg
2221 cctcggccat ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc atgtcggggg
2281 ggatccctcc agatcaacaa agattgatct ttgccggtaa gcagctagaa gacggtagaa
2341 cgctgtctga ttacaacatt cagaaggagt ccaccttaca tcttgtgcta aggctaagag
2401 gtggtatgca cagatcagct tgcggccgca tgaaagcgtt aacggccagg caacaagagg
2461 tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg cgtgcggaaa
2521 tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg aaggcgctgg
2581 cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggatccgt ctgttgcagg
2641 aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaacca cttctggcgc
2701 aacagcatat tgaaggtcat tatcaggtcg acccttcctt attcaagccg aatgctgatt
2761 tcctgctgcg cgtcagcggg atgtcgatga aagatatcgg cattatggat ggtgacttgc
2821 tggcagtgca taaaactcag gatgtacgta acggtcaggt cgttgtcgca cgtattgatg
2881 acgaagttac cgttaagggc ctgaaaaaac agggcaataa agtcgaactg ttgccagaaa
2941 atagcgagtt taaaccaatt gtcgtagatc ttcgtcagca gagcttcacc attgaagggc
3001 tggcggttgg ggttattcgc aacggcgact ggctggaatt cccggggatc tgggcccccc
3061 cgaccgatgt cagcctgggg gacgagctcc acttagacgg cgaggacgtg gcgatggcgc
3121 atgccgacgc gctagacgat ttcgatctgg acatgttggg ggacggggat tccccgggtc
3181 cgggatttac cccccacgac tccgccccct acggcgctct ggatatggcc gacttcgagt
3241 ttgagcagat gtttaccgat gcccttggaa ttgacgagta cggtgggtag gtcatgtaat
3301 tagttattca cgctacaacc ccc
//
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