质粒名称: | JD633 |
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出品公司: | Addgene |
目录编号: | 160393 |
存储实验室: | Jorge Dubcovsky |
载体骨架基本信息 |
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载体名称: | pTRANS_250d |
载体出品公司: | Daniel Voytas |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 双运载体 |
筛选标记: | 潮霉素;PvUbi2:hpt-II |
载体抗性: | 卡那霉素 |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | -- |
插入基因信息 |
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插入基因名称: | ZmUbi::wheat GRF4-GIF1 chimera |
物种来源: | Synthetic |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子: | -- |
标签/融合蛋白: | -- |
克隆方法: | -- |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | -- |
3’测序引物 | -- |
文献引用方法 |
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材料和方法部分: | JD633 was a gift from Jorge Dubcovsky (Addgene plasmid # 160393 ; http://n2t.net/addgene:160393 ; RRID:Addgene_160393) |
参考文献部分: | A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J. Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 10.1038/s41587-020-0703-0. 10.1038/s41587-020-0703-0 PubMed 33046875 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS JD633 21667 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning. ACCESSION . VERSION . KEYWORDS JD633 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 21667) AUTHORS Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J TITLE A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. JOURNAL Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 10.1038/s41587-020-0703-0. PUBMED 33046875 REFERENCE 2 (bases 1 to 21667) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..21667 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1233..1862 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 2291..3364 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 3430..3624 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(3860..3882) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 3968..4108 /label=bom /note="basis of mobility region from pBR322" primer_bind 4023..4042 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(4123..4140) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4294..4882) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4374..4393) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4969..5763) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 6188..6212 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" polyA_signal 6290..6464 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(6505..7530) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK K" protein_bind 9470..9491 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9506..9536 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9544..9560 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9568..9584 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 11629..11653 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 11650..11659 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(13568..13592) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 13621..13710 /codon_start=1 /product="three tandem HA epitope tags" /label=3xHA /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA" terminator 13744..13996 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" CDS 16022..20125 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 20126..20173 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS complement(20810..21115) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGTCL" primer_bind 20980..20996 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 21006..21025 /label=T7 /note="T7 promoter, forward primer" promoter 21006..21024 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(21072..21092) /label=T3 /note="T3 promoter, forward primer" promoter complement(21072..21090) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(21111..21127) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 21135..21151 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(21159..21189) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 21204..21225 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." misc_RNA 21264..21339 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" primer_bind complement(21356..21372) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 21575..21599 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" ORIGIN 1 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca 61 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa 121 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg 181 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca 241 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga 301 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt 361 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca 421 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg 481 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc 541 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg 601 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc 661 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca 721 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg 781 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga 841 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc 901 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa 961 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag 1021 ccgcccgcgc acggctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc 1081 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa 1141 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga 1201 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga 1261 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg 1321 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg 1381 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg 1441 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact 1501 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt 1561 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg 1621 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg 1681 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc 1741 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg 1801 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt 1861 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc 1921 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa 1981 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg 2041 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat 2101 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc 2161 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc 2221 caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg 2281 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg 2341 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc 2401 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg 2461 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc 2521 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga 2581 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga 2641 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga 2701 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga 2761 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt 2821 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt 2881 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac 2941 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg 3001 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa 3061 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg 3121 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa 3181 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg 3241 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc 3301 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc 3361 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg 3421 cctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa 3481 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga 3541 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct 3601 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagcccaag agctgcaaaa 3661 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc 3721 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca 3781 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt 3841 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggaaacggtc acagcttgtc 3901 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 3961 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 4021 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 4081 atgcgtaagg agaaaatacc gcatcaggcc ctcttccgct tcctcgctca ctgactcgct 4141 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 4201 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 4261 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 4321 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 4381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 4441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 4501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 4561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 4621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 4681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 4741 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 4801 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 4861 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 4921 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc 4981 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa 5041 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc 5101 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt 5161 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc 5221 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc 5281 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc 5341 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa 5401 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata 5461 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg 5521 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc 5581 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata 5641 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc 5701 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc 5761 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat 5821 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca 5881 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg 5941 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc 6001 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg 6061 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg 6121 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc 6181 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca 6241 cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt 6301 tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata 6361 caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc 6421 taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc 6481 gacagatccc ggtcggcatc tactctattt ctttgccctc ggacgagtgc tggggcgtcg 6541 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg 6601 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc 6661 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag 6721 accaatgcgg agcatatacg cccggagtcg tggcgatcct gcaagctccg gatgcctccg 6781 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt 6841 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat 6901 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac 6961 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact 7021 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat 7081 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct 7141 cgtctggcta agatcggccg cagcgatcgc atccatagcc tccgcgaccg gttgtagaac 7201 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgaac ggcgggagat 7261 gcaataggtc aggctctcgc taaactcccc aatgtcaagc acttccggaa tcgggagcgc 7321 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt 7381 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc 7441 ctccgagagc tgcatcaggt cggacacgct gtcgaacttt tcgatcagaa acttctcgac 7501 agacgtcgcg gtgagttcag gctttttcat aagcttctgc aaaagagaac cagacaacag 7561 ggtaagtgcc tagcagtaaa caaacagaac tcatcacaag caaacagcaa catcatattc 7621 ataccaacag gtcatgtgtg ttcatcacat cattagtact aagcatgcca tcatccaagt 7681 atatcaaagt aagggcaaag agcatgcatg atcatcaggt gcacaaaaga atcatcaaat 7741 tgtagcagta catatcttca tctatcatgc atatctatcc ataacaggac gatgcatgtt 7801 gaccaggtaa aagctacagg atcctatagg aacagcagcg tatatatctt caccaatctt 7861 agcatcttaa tcatgtggca catgcagttt caatttaagc acatgagcta gttgattatg 7921 aggtaccaga gaatcatcaa actgatgtag cagcatatat cctcatctat catgcaatct 7981 aatctaatct aactaaacag gaaaggtgtg ctattcagtt aaaagctacc gcatcatata 8041 caaacggcag cataagaaaa agcataatca tcttaatcat gcaacaaacg cagattcata 8101 ataagcccaa gagctagctt gtgatgatct tattctactc tgatctacag caatcagata 8161 acgacctaac cttgcacatg gcaacaaaac aatcgatcgg acgaatcagt tgtttgttcc 8221 tagctagcac catcgaacca gataatagat gcacgtacag atcccgaaaa cgaacccaaa 8281 aacagggcag acctagctga acctaggcag cgacccagca gatcgtgaga acgatctcat 8341 ctacgaacag cctagaagca accccacgat tcccggacaa acgacctaaa atccccacaa 8401 atcacatgag catgacagga taaacagcgg aaccgatcag atctacacga aaaccccacc 8461 tcccagccac ccacgatcag gaaacacgcg gatctagcat gatttcgtca acgcctcagc 8521 ctagttccta gccacagacc aagcagaacc accaaaccac gccgagcgag gagatggggc 8581 aagaggacgg gggagacgat cgccgtacct tgaagcgggg gaaggatcgc cgagggtcgc 8641 gaggagagca atttggattt ggaacccggg ggttgtgcgc tccgaacgat gagacgatgt 8701 gagattgtgg gaagaggcgc ggagggccct gtatttatgg gctgcgacgg ggggaggaga 8761 ggtggggagg gttggggaag gaatccccca cccgtgccgt gacggttccg ggccgtgtga 8821 gaggagcccg ctcgtctccg ccacgcaatt tccgcgatcg gagcggagct ttcgagaggc 8881 ggctggatgg ttggtggccg ttagatttgt agacgccgtt aacgcctcgc ctccaccggg 8941 aagagttttg agcagccgct tatgacaatg gcttaacgac gttagacgga gcgttagtgg 9001 caggccatga tagaatagac gtattgcaat gggattataa ttaaataaat aagaatataa 9061 taagatatgg caagtcggca ctcatgacat ggtcttcgaa atgatagtgc tcactttctt 9121 agccgagaaa gttgacgcga ctgatttaga agttaagata ttatttctct cttcttttct 9181 ttcctcgtca tataaggatg aaataaactt tagagattgc cggtagtgat tttggatttc 9241 ggcgatcagg cttggtttgc cggtttcgga cggtgtgcct taggccaccc gcagtgtatc 9301 ttgtaatgtt caaccgataa gcaagggtgg ggctcaagca agtagtaaac aactatgtca 9361 aatgtcacca tggttatggt cttgtttagt tggcttcttg gccgattcat taatgcagct 9421 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 9481 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 9541 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga 9601 gctcggtacc cggggatctg caggcgcgtg cagcgtgacc cggtcgtgcc cctctctaga 9661 gataatgagc attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact 9721 tgtttgaagt gcagtttatc tatctttata catatattta aactttactc tacgaataat 9781 ataatctata gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag 9841 acatggtcta aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta 9901 gtgtgcatgt gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat 9961 tttattagta catccattta gggtttaggg ttaatggttt ttatagacta atttttttag 10021 tacatctatt ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt 10081 tttttattta ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa 10141 atacccttta agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg 10201 ccagcctgtt aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc 10261 gcgtcgggcc aagcgaagca gacggcacgg catctctgtc gctgcctctg gacccctctc 10321 gagagttccg ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg 10381 agcggcagac gtgagccggc acggcaggcg gcctcctcct cctctcacgg caccggcagc 10441 tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat 10501 agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac 10561 aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc 10621 tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg 10681 gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct 10741 agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt 10801 gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat 10861 gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg 10921 ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg 10981 tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac tacctggtgg 11041 atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga 11101 tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc 11161 atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt 11221 tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt 11281 tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata 11341 tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata catgatggca 11401 tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta 11461 tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg 11521 tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc 11581 gatgctcacc ctgttgtttg gtgttacttc tgcagactag agttatcaac aagtttgtac 11641 aaaaaagctg ccaccatggc gatgccgtat gcctctcttt ccccggcagg cgaccgccgc 11701 tcctccccgg ccgccaccgc caccgcctcc ctcctcccct tctgccgctc ctcccccttc 11761 tccgccggcg gcaatggcgg catgggggag gaggcgcgga tggacgggag gtggatggcg 11821 aggccggtgc ccttcacggc ggcgcagtac gaggagctgg agcaccaggc gctcatatac 11881 aagtacctgg tggccggcgt gtccgtcccg ccggatctcg tgctccccat ccgccgcggc 11941 atcgagtccc tcgccgcccg cttctaccac aaccccctcg ccatcgggta cggatcgtac 12001 ctgggcaaga aggtggatcc ggagccgggc cggtgccggc gcacggacgg caagaagtgg 12061 cggtgcgcca aggaggccgc ctccgactcc aagtattgcg agcgccacat gcaccgcggc 12121 cgcaaccgtt caagaaagcc tgtggaaacg cagctcgtct cgcactccca gccgccggcc 12181 gcctccgtcg tgccgcccct cgccaccggc ttccacaacc actccctcta ccccgccatc 12241 ggcggcacca acggtggtgg aggcgggggg aacaacggca tgtccatgcc cggcacgttc 12301 tcctccgcgc tggggccgcc tcagcagcac atgggcaaca atgccgcctc tccctacgcg 12361 gctctcggcg gcgccggaac atgcaaagat ttcaggtata ccgcatatgg aataagatct 12421 ttggcagacg agcagagtca gctcatgaca gaagccatga acacctccgt ggagaaccca 12481 tggcgcctgc cgccatcttc tcaaacgact acattcccgc tctcaagcta ctctcctcag 12541 cttggagcaa cgagtgacct gggtcagaac aacagcagca acaacaacag cggcgtcaag 12601 gccgagggac agcagcagca gcagccgctc tccttcccgg ggtgcggcga cttcggcagc 12661 ggcgactccg cgaagcagga gaaccagacg ctgcggccgt tcttcgacga gtggccgaag 12721 acgagggact cgtggtcgga cctgaccgac gacaactcga acgtcgcctc cttctcggcc 12781 acccagctgt cgatctcgat acccatgacg tcctccgact tctccgccgc cagctcccag 12841 tcgcccaacg gcatgctgtt cgccggcgaa atgtacgcgg ccgctgccat gcagcagcaa 12901 cacctgatgc agatgaacca gagcatgatg gggggctacg cttcctctac cactgccacc 12961 actgatctca ttcagcagta cctggatgag aacaagcagc tgatcctggc catcctcgac 13021 aaccagaaca acggcaaggt ggaggagtgc gcacggaacc aagctaagct ccagcagaac 13081 ctcatgtacc tcgccgccat cgccgacagc cagcctccgc agacggcatc gctgtctcag 13141 tacccgtcca acctgatgat gcagtccggg ccgcggtaca tgcagcagca gtcggcgcag 13201 atgatgtcgc cgcagtcgct gatggcggcg cggtcgtcga tgatgtacgc gcagcaggcc 13261 atgtcgccgc tccagcagca gcagcagcag cagcagcacc aggcggccgc gcacggccag 13321 ctggggatgt cctccggcgc gaccaccggg ttcaacctcc tgcacggtga ggccagcatg 13381 ggcggcggcg gcggcgccac tggcaacagc atgatgaacg ccagcgtctt ctcggactat 13441 ggccgcggcg gcagcggcgc caaggagggg tcgacctcgc tgtcggccga cgctcgcggc 13501 gccaactctg gcgcgcacag cggcgacggg gagtacctca agggcaccga ggaggaagga 13561 agctagcgtt cagctttctt gtacaaagtg gttgataaca gcgggttaat taacatcttt 13621 tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga 13681 tcctatccat atgacgttcc agattacgct gctcagtgca gcgcttagag ctcgaatttc 13741 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt 13801 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa 13861 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa 13921 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca 13981 tctatgttac tagatcgggg attagcgcgc ctgcagtgca gcgtgacccg gtcgtgcccc 14041 tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca tatttttttt 14101 gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa ctttactcta 14161 cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc atataaatga 14221 acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc tacagtttta 14281 tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct atataatact 14341 tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt atagactaat 14401 ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac taaaactcta 14461 ttttagtttt tttatttaat aatttagata taaaatagaa taaaataaag tgactaaaaa 14521 ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct tgtttcgagt 14581 agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac cagcgaacca 14641 gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc tgcctctgga 14701 cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat ccagaaattg 14761 cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc tctcacggca 14821 ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc tcgcccgccg 14881 taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac 14941 acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc aaggtacgcc 15001 gctcgtcctc cccccccccc ctctctacct tctctagatc ggcgttccgg tccatggtta 15061 gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg 15121 tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact 15181 tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg 15241 atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca 15301 atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt 15361 gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattaatt ctgtttcaaa 15421 ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata ttcatagtta 15481 cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt 15541 tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt 15601 tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact acctggtgta 15661 tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg agtttaagat 15721 ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat 15781 acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct atctattata 15841 ataaacaagt atgttttata attattttga tcttgatata cttggatgat ggcatatgca 15901 gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg cttggtactg 15961 tttcttttgt cgatgctcac cctgttgttt ggtgttactt cctgcagggc gatctattcg 16021 aatggacaag aagtactcga tcggcctcga catcgggacg aactcagttg gctgggccgt 16081 gatcaccgac gagtacaagg tgccctctaa gaagttcaag gtcctgggga acaccgaccg 16141 ccattccatc aagaagaacc tcatcggcgc tctcctgttc gacagcgggg agaccgctga 16201 ggctacgagg ctcaagagaa ccgctaggcg ccggtacacg agaaggaaga acaggatctg 16261 ctacctccaa gagattttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg 16321 cctggaggag tctttcctcg tggaggagga taagaagcac gagcggcatc ccatcttcgg 16381 caacatcgtg gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa 16441 gaagctcgtg gactccaccg ataaggcgga cctcagactg atctacctcg ctctggccca 16501 catgatcaag ttccgcggcc atttcctgat cgagggggat ctcaacccag acaacagcga 16561 tgttgacaag ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc 16621 gatcaacgcc tctggcgtgg acgcgaaggc tatcctgtcc gcgaggctct cgaagtccag 16681 gaggctggag aacctgatcg ctcagctccc aggcgagaag aagaacggcc tgttcgggaa 16741 cctcatcgct ctcagcctgg ggctcacccc gaacttcaag tcgaacttcg atctcgctga 16801 ggacgccaag ctgcaactct ccaaggacac ctacgacgat gacctcgata acctcctggc 16861 ccagatcggc gatcaatacg cggacctgtt cctcgctgcc aagaacctgt cggacgccat 16921 cctcctgtca gatatcctcc gcgtgaacac cgagatcacg aaggctccac tctctgcctc 16981 catgatcaag cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg 17041 ccaacagctc ccggagaagt acaaggagat tttcttcgat cagtcgaaga acggctacgc 17101 tgggtacatc gacggcgggg cctcacaaga ggagttctac aagttcatca agccaatcct 17161 ggagaagatg gacggcacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg 17221 gaagcagaga accttcgata acggcagcat cccccaccaa atccatctcg gggagctgca 17281 cgccatcctg agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat 17341 cgagaagatc ctgaccttca gaatcccata ctacgtcggc cctctcgcgc gggggaactc 17401 aagattcgct tggatgaccc gcaagtctga ggagaccatc acgccgtgga acttcgagga 17461 ggtggtggac aagggcgcta gcgctcagtc gttcatcgag aggatgacca acttcgacaa 17521 gaacctgccc aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt 17581 ctacaacgag ctcacgaagg tgaagtacgt caccgagggc atgcgcaagc cagcgttcct 17641 gtccggggag cagaagaagg ctatcgtgga cctcctgttc aagaccaacc ggaaggtcac 17701 ggttaagcaa ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtcgagat 17761 cagcggcgtt gaggaccgct tcaacgccag cctcgggacc taccacgatc tcctgaagat 17821 catcaaggat aaggacttcc tggacaacga ggagaacgag gatatcctgg aggacatcgt 17881 gctgaccctc acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc 17941 ccatctcttc gatgacaagg tcatgaagca actcaagcgc cggagataca ccggctgggg 18001 gaggctgtcc cgcaagctca tcaacggcat ccgggacaag cagtccggga agaccatcct 18061 cgacttcctg aagagcgatg gcttcgccaa caggaacttc atgcaactga tccacgatga 18121 cagcctcacc ttcaaggagg atatccaaaa ggctcaagtg agcggccagg gggactcgct 18181 gcacgagcat atcgcgaacc tcgctggctc ccccgcgatc aagaagggca tcctccagac 18241 cgtgaaggtt gtggacgagc tcgtgaaggt catgggccgg cacaagcctg agaacatcgt 18301 catcgagatg gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg 18361 catgaagcgc atcgaggagg gcatcaagga gctggggtcc caaatcctca aggagcaccc 18421 agtggagaac acccaactgc agaacgagaa gctctacctg tactacctcc agaacggcag 18481 ggatatgtac gtggaccaag agctggatat caaccgcctc agcgattacg acgtcgatca 18541 tatcgttccc cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc 18601 ggacaagaac cgcggcaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa 18661 gaactactgg aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct 18721 caccaaggct gagagaggcg ggctctcaga gctggacaag gccggcttca tcaagcggca 18781 gctggtcgag accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa 18841 cacgaagtac gatgagaacg acaagctgat cagggaggtt aaggtgatca ccctgaagtc 18901 taagctcgtc tccgacttca ggaaggattt ccagttctac aaggttcgcg agatcaacaa 18961 ctaccaccat gcccatgacg cttacctcaa cgctgtggtc ggcaccgctc tgatcaagaa 19021 gtacccaaag ctggagtccg agttcgtgta cggggactac aaggtttacg atgtgcgcaa 19081 gatgatcgcc aagtcggagc aagagatcgg caaggctacc gccaagtact tcttctactc 19141 aaacatcatg aacttcttca agaccgagat cacgctggcc aacggcgaga tccggaagag 19201 accgctcatc gagaccaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt 19261 cgcgaccgtc cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt 19321 ccaaacgggc gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat 19381 cgccagaaag aaggactggg acccaaagaa gtacggcggg ttcgacagcc ctaccgtggc 19441 ctactcggtc ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt 19501 gaaggagctc ctggggatca ccatcatgga gaggtccagc ttcgagaaga acccaatcga 19561 cttcctggag gccaagggct acaaggaggt gaagaaggac ctgatcatca agctcccgaa 19621 gtactctctc ttcgagctgg agaacggcag gaagagaatg ctggcttccg ctggcgagct 19681 ccagaagggg aacgagctcg cgctgccaag caagtacgtg aacttcctct acctggcttc 19741 ccactacgag aagctcaagg gcagcccgga ggacaacgag caaaagcagc tgttcgtcga 19801 gcagcacaag cattacctcg acgagatcat cgagcaaatc tccgagttca gcaagcgcgt 19861 gatcctcgcc gacgcgaacc tggataaggt cctctccgcc tacaacaagc accgggacaa 19921 gcccatcaga gagcaagcgg agaacatcat ccatctcttc accctgacga acctcggcgc 19981 tcctgctgct ttcaagtact tcgacaccac gatcgatcgg aagagataca cctccacgaa 20041 ggaggtcctg gacgcgaccc tcatccacca gtcgatcacc ggcctgtacg agacgaggat 20101 cgacctctca caactcggcg gggataagag acccgcagca accaagaagg cagggcaagc 20161 aaagaagaag aagtgactcg agatatgaag atgaagatga aatatttggt gtgtcaaata 20221 aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg 20281 ctttttctaa tattaaatga atgtaagatc acattataat gaataaacaa atgtttctat 20341 aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat 20401 ggtatggact atggaatatg attaaagata aggagctccg gtgacggacg accaagcccg 20461 ttattctgac agttctggtg ctcaacacat ttatatttat caaggagcac attgttactc 20521 actgctagga gggaatcgaa ctaggaatat tgatcagagg aactacgaga gagctgaaga 20581 taactgccct ctagctctca ctgatctggg tcgcatagtg agatgcagcc cacgtgagtt 20641 cagcaacggt ctagcgctgg gcttttaggc ccgcatgatc gggcttttgt cgggtggtcg 20701 acgtgttcac gattggggag agcaacgcag cagttcctct tagtttagtc ccacctcgcc 20761 tgtccagcag agttctgacc ggtttataaa ctcgcttgct gcatcagact tagaggcagg 20821 tgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat 20881 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 20941 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc 21001 gcgcgtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcctcc 21061 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg 21121 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 21181 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 21241 ctgcccgctt tccacctgcg gtcgttttag agctagaaat agcaagttaa aataaggcta 21301 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttcccg gtggcactgg 21361 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 21421 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 21481 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg 21541 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc 21601 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat 21661 ccgttcg //
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