pRK415

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: --
载体名称: pRK415
质粒类型: 广宿主质粒
高拷贝/低拷贝: --
复制子: oriV
启动子: Lac/lac
载体大小: 10690bp
5' 测序引物及序列: M13R:CAGGAAACAGCTATGACC
3' 测序引物及序列: M13F:TGTAAAACGACGGCCAGT
载体标签: traJ,LacZ
载体抗性: Tet
筛选标记: --
克隆菌株: DH5α 等
宿主细胞(系): --
备注: --
产品目录号: --
稳定性: --
组成型/诱导型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pRK415载体图谱
pRK415载体图谱1

载体简介

载体序列

LOCUS       pRK415               10690 bp ds-DNA     circular SYN 06-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pRK415
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, September 6, 2016 from SnapGene Viewer 3.1.4
            http://www.biofeng.com/
FEATURES             Location/Qualifiers
     source          1..10690
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..712
                     /note="oriV"
                     /note="incP origin of replication"
     CDS             complement(971..1312)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /note="lacZ-alpha"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRHASRREKGGQVSGKRQGRNRRAHEGA
                     SRGKRLVSL"
     primer_bind     1222..1238
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1239..1295
                     /note="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1308..1324)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1332..1348
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1356..1386)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1401..1422
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(1654..2025)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /note="traJ"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            2058..2167
                     /note="incP origin of transfer"
     CDS             complement(2848..3498)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /note="TetR"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             3604..4803
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /note="TcR"
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(6074..7222)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /note="trfA"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
       61 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
      121 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
      181 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
      241 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
      301 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
      361 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
      421 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
      481 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
      541 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
      601 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
      661 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gctcttccgc
      721 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      781 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      841 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      901 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      961 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1021 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1081 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     1141 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     1201 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtacccggg
     1261 gatcctctag agtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt
     1321 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1381 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1441 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1501 gggagaggcg gtttgcgtat tgggcgctcg gtcttgcctt gctcgtcggt gatgtacttc
     1561 accagctccg cgaagtcgct cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg
     1621 cgcacccccc ggccgtttta gcggctaaaa aagtcatggc tctgccctcg ggcggaccac
     1681 gcccatcatg accttgccaa gctcgtcctg cttctcttcg atcttcgcca gcagggcgag
     1741 gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag
     1801 gccgcccagg cggcccaggt cgccattgat gcgggccagc tcgcggacgt gctcatagtc
     1861 cacgacgccc gtgattttgt agccctggcc gacggccagc aggtaggccg acaggctcat
     1921 gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt
     1981 gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc
     2041 tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag
     2101 gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat
     2161 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa
     2221 tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa
     2281 gcagggttat gcagcggaaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2341 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2401 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2461 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2521 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2581 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2641 gcagcgagtc agtgagcgag gaagcggaag agcgccagaa ggccgccaga gaggccgagc
     2701 gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa gcccgtagcg ggctgctacg
     2761 ggcgtctgac gcggtggaaa gggggagggg atgttgtcta catggctctg ctgtagtgag
     2821 tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc tttctcggtc cttcaacgtt
     2881 cctgacaacg agcctccttt tcgccaatcc atcgacaatc accgcgagtc cctgctcgaa
     2941 cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg gcccgcaaca gcggcgagag
     3001 cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg ctgtcgccgg cctgctcctc
     3061 aagcacggcc ccaacagtga agtagctgat tgtcatcagc gcattgacgg cgtccccggc
     3121 cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc
     3181 gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct
     3241 gaagctgcgg gcattcccga tcagaaatga gcgccagtcg tcgtcggctc tcggcaccga
     3301 atgcgtatga ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt
     3361 cctgaagtgc cagtaaagcg ccggctgctg aacccccaac cgttccgcca gtttgcgtgt
     3421 cgtcagaccg tctacgccga cctcgttcaa caggtccagg gcggcacgga tcactgtatt
     3481 cggctgcaac tttgtcatgc ttgacacttt atcactgata aacataatat gtccaccaac
     3541 ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca
     3601 gacatgaaac ccaacatacc cctgatcgta attctgagca ctgtcgcgct cgacgctgtc
     3661 ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac
     3721 gacgtcaccg cccactatgg cattctgctg gcgctgtatg cgttggtgca atttgcctgc
     3781 gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc ggccaatctt gctcgtctcg
     3841 ctggccggcg ccactgtcga ctacgccatc atggcgacag cgcctttcct ttgggttctc
     3901 tatatcgggc ggatcgtggc cggcatcacc ggggcgactg gggcggtagc cggcgcttat
     3961 attgccgata tcactgatgg cgatgagcgc gcgcggcact tcggcttcat gagcgcctgt
     4021 ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg tttctccccc
     4081 cacgctccgt tcttcgccgc ggcagccttg aacggcctca atttcctgac gggctgtttc
     4141 cttttgccgg agtcgcacaa aggcgaacgc cggccgttac gccgggaggc tctcaacccg
     4201 ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg ccgccctgat ggcggtcttc
     4261 ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt cggcgaggat
     4321 cgctttcact gggacgcgac cacgatcggc atttcgcttg ccgcatttgg cattctgcat
     4381 tcactcgccc aggcaatgat caccggccct gtagccgccc ggctcggcga aaggcgggca
     4441 ctcatgctcg gaatgattgc cgacggcaca ggctacatcc tgcttgcctt cgcgacacgg
     4501 ggatggatgg cgttcccgat catggtcctg cttgcttcgg gtggcatcgg aatgccggcg
     4561 ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc aggggcagct gcaaggctca
     4621 ctggcggcgc tcaccagcct gacctcgatc gtcggacccc tcctcttcac ggcgatctat
     4681 gcggcttcta taacaacgtg gaacgggtgg gcatggattg caggcgctgc cctctacttg
     4741 ctctgcctgc cggcgctgcg tcgcgggctt tggagcggcg cagggcaacg agccgatcgc
     4801 tgatcgtgga aacgataggc ctatgccatg cgggtcaagg cgacttccgg caagctatac
     4861 gcgccctagg agtgcggttg gaacgttggc ccagccagat actcccgatc acgagcagga
     4921 cgccgatgat ttgaagcgca ctcagcgtct gatccaagaa caaccatcct agcaacacgg
     4981 cggtccccgg gctgagaaag cccagtaagg aaacaactgt aggttcgagt cgcgagatcc
     5041 cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac gccaggccga
     5101 gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct actggaacga
     5161 gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc acgggaggtt
     5221 gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc aggcccgctg
     5281 cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag
     5341 ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc agagcggcag
     5401 agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc cgcccggcag
     5461 gcggtagacc gaaataaaca acaagctcca gaatagcgaa atattaagtg cgccgaggat
     5521 gaagatgcgc atccaccaga ttcccgttgg aatctgtcgg acgatcatca cgagcaataa
     5581 acccgccggc aacgcccgca gcagcatacc ggcgacccct cggcctcgct gttcgggctc
     5641 cacgaaaacg ccggacagat gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa
     5701 gaccgacagc ccaatgatct cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa
     5761 ccgttcagcg aacgcctcca tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat
     5821 ctctggagct ttcttcaggg ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc
     5881 tccaagccgt cgaatctgag ccttaatcac aattgtcaat tttaatcctc tgtttatcgg
     5941 cagttcgtag agcgcgccgt gcgcccgagc gatactgagc gaagcaagtg cgtcgagcag
     6001 tgcccgcttg ttcctgaaat gccagtaaag cgctggctgc tgaaccccca gccggaactg
     6061 accccacaag gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca
     6121 ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca
     6181 cgcgggtgga atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct
     6241 cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact
     6301 tctcccatat gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga
     6361 tcaggacctg gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca
     6421 gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta
     6481 ggcgcgacag gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt
     6541 cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt
     6601 actccaacac ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt
     6661 aggtgatctt cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg
     6721 ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc
     6781 gcatcgtgtc cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct
     6841 gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct gacctgtttt gccaggtcct
     6901 cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact
     6961 tcgccaaacc tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg gccgatggcg
     7021 cgggcagggc agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct
     7081 ggaccatcga gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg
     7141 cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct
     7201 tccggtcaaa cgtccgattc attcaccctc cttgcgggat tgccccgact cacgccgggg
     7261 caatgtgccc ttattcctga tttgacccgc ctggtgcctt ggtgtccaga taatccacct
     7321 tatcggcaat gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac ttggtattcc
     7381 gaatcttgcc ctgcacgaat accagcgacc ccttgcccaa atacttgccg tgggcctcgg
     7441 cctgagagcc aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat
     7501 cgttgcgcca ctcttcatta accgctatat cgaaaattgc ttgcggcttg ttagaattgc
     7561 catgacgtac ctcggtgtca cgggtaagat taccgataaa ctggaactga ttatggctca
     7621 tatcgaaagt ctccttgaga aaggagactc tagtttagct aaacattggt tccgctgtca
     7681 agaactttag cggctaaaat tttgcgggcc gcgaccaaag gtgcgagggg cggcttccgc
     7741 tgtgtacaac cagatatttt tcaccaacat ccttcgtctg ctcgatgagc ggggcatgac
     7801 gaaacatgag ctgtcggaga gggcaggggt ttcaatttcg tttttatcag acttaaccaa
     7861 cggtaaggcc aacccctcgt tgaaggtgat ggaggccatt gccgacgccc tggaaactcc
     7921 cctacctctt ctcctggagt ccaccgacct tgaccgcgag gcactcgcgg agattgcggg
     7981 tcatcctttc aagagcagcg tgccgcccgg atacgaacgc atcagtgtgg ttttgccgtc
     8041 acataaggcg tttatcgtaa agaaatgggg cgacgacacc cgaaaaaagc tgcgtggaag
     8101 gctctgacgc caagggttag ggcttgcact tccttcttta gccgctaaaa cggccccttc
     8161 tctgcgggcc gtcggctcgc gcatcatatc gacatcctca acggaagccg tgccgcgaat
     8221 ggcatcgggc gggtgcgctt tgacagttgt tttctatcag aacccctacg tcgtgcggtt
     8281 cgattagctg tttgtcttgc aggctaaaca ctttcggtat atcgtttgcc tgtgcgataa
     8341 tgttgctaat gatttgttgc gtaggggtta ctgaaaagtg agcgggaaag aagagtttca
     8401 gaccatcaag gagcgggcca agcgcaagct ggaacgcgac atgggtgcgg acctgttggc
     8461 cgcgctcaac gacccgaaaa ccgttgaagt catgctcaac gcggacggca aggtgtggca
     8521 cgaacgcctt ggcgagccga tgcggtacat ctgcgacatg cggcccagcc agtcgcaggc
     8581 gattatagaa acggtggccg gattccacgg caaagaggtc acgcggcatt cgcccatcct
     8641 ggaaggcgag ttccccttgg atggcagccg ctttgccggc caattgccgc cggtcgtggc
     8701 cgcgccaacc tttgcgatcc gcaagcgcgc ggtcgccatc ttcacgctgg aacagtacgt
     8761 cgaggcgggc atcatgaccc gcgagcaata cgaggtcatt aaaagcgccg tgattgatga
     8821 tatagcggcc cggctgctcc tggttctcgc gcaccgaaat gggtgacttc accccgcgct
     8881 ctttgatcgt ggcaccgatt tccgcgatgc tctccgggga aaagccgggg ttgtcggccg
     8941 tccgcggctg atgcggatct tcgtcgatca ggtccaggtc cagctcgata gggccggaac
     9001 cgccctgaga cgccgcagga gcgtccagga ggctcgacag gtcgccgatg ctatccaacc
     9061 ccaggccgga cggctgcgcc gcgcctgcgg cttcctgagc ggccgcagcg gtgtttttct
     9121 tggtggtctt ggcttgagcc gcagtcattg ggaaatctcc atcttcgtga acacgtaatc
     9181 agccagggcg cgaacctctt tcgatgcctt gcgcgcggcc gttttcttga tcttccagac
     9241 cggcacaccg gatgcgaggg catcggcgat gctgctgcgc aggccaacgg tggccggaat
     9301 catcatcttg gggtacgcgg ccagcagctc ggcttggtgg cgcgcgtggc gcggattccg
     9361 cgcatcgacc ttgctgggca ccatgccaag gaattgcagc ttggcgttct tctggcgcac
     9421 gttcgcaatg gtcgtgacca tcttcttgat gccctggatg ctgtacgcct caagctcgat
     9481 gggggacagc acatagtcgg ccgcgaagag ggcggccgcc aggccgacgc caagggtcgg
     9541 ggccgtgtcg atcaggcaca cgtcgaagcc ttggttcgcc agggccttga tgttcgcccc
     9601 gaacagctcg cgggcgtcgt ccagcgacag ccgttcggcg ttcgccagta ccgggttgga
     9661 ctcgatgagg gcgaggcgcg cggcctggcc gtcgccggct gcgggtgcgg tttcggtcca
     9721 gccgccggca gggacagcgc cgaacagctt gcttgcatgc aggccggtag caaagtcctt
     9781 gagcgtgtag gacgcattgc cctgggggtc caggtcgatc acggcaaccc gcaagccgcg
     9841 ctcgaaaaag tcgaaggcaa gatgcacaag ggtcgaagtc ttgccgacgc cgcctttctg
     9901 gttggccgtg accaaagttt tcatcgtttg gtttcctgtt ttttcttggc gtccgcttcc
     9961 cacttccgga cgatgtacgc ctgatgttcc ggcagaaccg ccgttacccg cgcgtacccc
    10021 tcgggcaagt tcttgtcctc gaacgcggcc cacacgcgat gcaccgcttg cgacactgcg
    10081 cccctggtca gtcccagcga cgttgcgaac gtcgcctgtg gcttcccatc gactaagacg
    10141 ccccgcgcta tctcgatggt ctgctgcccc acttccagcc cctggatcgc ctcctggaac
    10201 tggctttcgg taagccgttt cttcatggat aacacccata atttgctccg cgccttggtt
    10261 gaacatagcg gtgacagccg ccagcacatg agagaagttt agctaaacat ttctcgcacg
    10321 tcaacacctt tagccgctaa aactcgtcct tggcgtaaca aaacaaaagc ccggaaaccg
    10381 ggctttcgtc tcttgccgct tatggctctg cacccggctc catcaccaac aggtcgcgca
    10441 cgcgcttcac tcggttgcgg atcgacactg ccagcccaac aaagccggtt gccgccgccg
    10501 ccaggatcgc gccgatgatg ccggccacac cggccatcgc ccaccaggtc gccgccctca
    10561 ctgcccggca cctggtcgct gaatgtcgat gccagcacct gcggcacgtc aatgcttccg
    10621 ggcgtcgcgc tcgggctgat cgcccatccc gttactgccc cgatcccggc aatggcaagg
    10681 actgccagcg
//

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