出品公司: | -- |
---|---|
载体名称: | pG-Host9, pGhost9, pG+host9 |
质粒类型: | 革兰氏阳性菌载体,广宿主 |
高拷贝/低拷贝: | -- |
复制子: | repA |
启动子: | -- |
载体大小: | 3752 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 红霉素Erm 200μg/ ml |
筛选标记: | -- |
克隆菌株: | MC1061 |
宿主细胞(系): | -- |
备注: | pG+HOST9质粒是乳酸菌和其他革兰氏阳性菌的温敏型整合载体。 |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | -- |
病毒/非病毒: | -- |
买家导航
pG-Host9载体 pGHost9质粒基本信息
pG-Host9质粒图谱 pGHost9载体图谱和pG-Host9载体序列 pGHost9质粒序列多克隆位点信息
pG-Host9质粒 pGHost9载体简介
pG-Host9质粒序列 pGHost9载体序列
LOCUS pG-Host9 3752 bp ds-DNA circular SYN 21-SEP-2017 DEFINITION synthetic circular DNA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3752) TITLE Direct Submission JOURNAL Exported Sep 21, 2017 from MiaoLingPlasmid http://www.biofeng.com/ FEATURES Location/Qualifiers source 1..3752 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(151..888) /codon_start=1 /label=Erm /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN LSTVTYEQVLSIFNSYLLFNGRK" CDS 2216..2914 /codon_start=1 /label=repA /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK VLDAETGEIK" promoter 3647..3665 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 3674..3751 /label=MCS ORIGIN 1 ctagagcggc cgccaccgcg gtgggatcct ctagagtccg ctagggacct ctttagctcc 61 ttggaagctg tcagtagtat acctaataat ttatctacat tccctttagt aacgtgtaac 121 tttccaaatt tacaaaagcg actcatagaa ttatttcctc ccgttaaata atagataact 181 attaaaaata gacaatactt gctcataagt aacggtactt aaattgttta ctttggcgtg 241 tttcattgct tgatgaaact gatttttagt aaacagttga cgatattctc gattgaccca 301 ttttgaaaca aagtacgtat atagcttcca atatttatct ggaacatctg tggtatggcg 361 ggtaagtttt attaagacac tgtttacttt tggtttagga tgaaagcatt ccgctggcag 421 cttaagcaat tgctgaatcg agacttgagt gtgcaagagc aaccctagtg ttcggtgaat 481 atccaaggta cgcttgtaga atccttcttc aacaatcaga tagatgtcag acgcatggct 541 ttcaaaaacc acttttttaa taatttgtgt gcttaaatgg taaggaatac tcccaacaat 601 tttatacctc tgtttgttag ggaattgaaa ctgtagaata tcttggtgaa ttaaagtgac 661 acgagtattc agttttaatt tttctgacga taagttgaat agatgactgt ctaattcaat 721 agacgttacc tgtttactta ttttagccag tttcgtcgtt aaatgccctt tacctgttcc 781 aatttcgtaa acggtatcgg tttcttttaa attcaattgt tttattattt ggttgagtac 841 tttttcactc gttaaaaagt tttgagaata ttttatattt ttgttcatgt aatcactcct 901 tcttaattac aaatttttag catctaattt aacttcaatt cctattatac aaaattttaa 961 gatactgcac tatcaacaca ctcttaagtt tgcttctaag tcttatttcc ataacttctt 1021 ttacgtttcc gccattcttt gctgtttcga tttttatgat atggtgcaag tcagcacgaa 1081 cacgaaccgt cttatctccc attatatctt tttttgcact gattggtgta tcatttcgtt 1141 tttcttttgc gcgactctag aggatcctga taaatatgaa catgatgagt gatcgttaaa 1201 tttatactgc aatcggatgc gattattgaa taaaagatat gagagattta tctaatttct 1261 tttttcttgt aaaaaaagaa agttcttaaa ggttttatag ttttggtcgt agagcacacg 1321 gtttaacgac ttaattacga agtaaataag tctagtgtgt tagactttat gaaatctata 1381 tacgtttata tatatttatt atccgatttt ttattaaaac gtctcaaaat cgtttctgag 1441 acgttttagc gtttatttcg tttagttatc ggcataatcg ttaaaacagg cgttatcgta 1501 gcgtaaaagc ccttgagcgt agcgtggctt tgcagcgaag atgttgtctg ttagattatg 1561 aaagccgatg actgaatgaa ataataagcg cagcgccctt ctatttcggt tggaggaggc 1621 tcaagggagt atgagggaat gaaattccct catgggtttg attttaaaaa ttgcttgcaa 1681 ttttgccgag cggtagcgct ggaaaatttt tgaaaaaaat ttggaatttg gaaaaaaatg 1741 gggggaaagg aagcgaattt tgcttccgta ctacgacccc ccattaagtg ccgagtgcca 1801 atttttgtgc caaaaacgct ctatcccaac tggctcaagg gtttaagggg tttttcaatc 1861 gccaacgaat cgccaacgtt ttcgccaacg ttttttataa atctatattt aagtagcttt 1921 attgttgttt ttatgattac aaagtgatac actaacttta taaaattatt tgattggagt 1981 tttttaaatg gtgatttcag aatcgaaaaa aagagttatg atttctctga caaaagagca 2041 agataaaaaa ttaacagata tggcgaaaca aaaaggtttt tcaaaatctg cggttgcggc 2101 gttagctata gaagaatatg caagaaagga atcagaacaa aaaaaataag cgaaagctcg 2161 cgtttttaga aggatacgag ttttcgctac ttgtttttga taaggtaatt atatcatggc 2221 tattaaaaat actaaagcta gaaattttgg atttttatta tatcctgact caattcctaa 2281 tgattggaaa gaaaaattag agagtttggg cgtatctatg gctgtcagtc ctttacacga 2341 tatggacgaa aaaaaagata aagatacatg gaataatagt aatattatac aaaatggaaa 2401 gcactataaa aaaccacact atcacgttat atatattgca cgaaatcctg taacaataga 2461 aagcgttagg aacaagatta agcgaaaatt ggggaatagt tcagttgctc atgttgagat 2521 acttgattat atcaaaggtt catatgaata tttgactcat gaatcaaagg acgctattgc 2581 taagaataaa catatatacg acaaaaaaga tattttgaac attaatgatt ttgatattga 2641 ccgctatata acacttgatg aaagccaaaa aagagaattg aagaatttac ttttagatat 2701 agtggatgac tataatttgg taaatacaaa agatttaatg gcttttattc gccttagggg 2761 agcggagttt ggaattttaa atacgaatga tgtaaaagat attgtttcaa caaactctag 2821 cgcctttaga ttatggtttg agggcaatta tcagtgtgga tatagagcaa gttatgcaaa 2881 ggttcttgat gctgaaacgg gggaaataaa atgacaaaca aagaaaaaga gttatttgct 2941 gaaaatgagg aattaaaaaa agaaattaag gacttaaaag agcgtattga aagatacaga 3001 gaaatggaag ttgaattaag tacaacaata gatttattga gaggagggat tattgaataa 3061 ataaaagccc cctgacgaaa gtcgaagggg gtttttattt tggtttgatg ttgcgattaa 3121 tagcaataca attgcaataa acaaaatgat cttccttcag gttatgacca tctgtgccag 3181 ttcgtaatgt ctggtcaact ttccgactct gagaaacttc tggaatcgct agagaatttc 3241 tggaatggga ttcaggagtg gacagaacga cacggatata tagtggatgt gtcaaaacgc 3301 ataccatttt gaacgatgac ctctaataat tgttaatcat gttggttacg tatttattaa 3361 cttctcctag tattagtaat tatcatggct gtcatggcgc attaacggaa taaagggtgt 3421 gcttaaatcg ggccattttg cgtaataaga aaaaggatta attatgagcg aattgaatta 3481 ataataaggt aatagattta cattagaaaa tgaaagggga ttttatgcgt gagaatgtta 3541 cagtctatcc ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3601 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact 3661 atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat 3721 cgaattcctg cagcccgggg gatccactag tt //
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