| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.77 ; pGL4.77[hRlucP/Hygro] |
| 质粒类型: | 信号通路报告载体；哺乳动物载体；海肾荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 5252 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | hRlucP |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 潮霉素（Hygromycin） |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | HEK293等 |
| 备注: | pGL4.77[hRlucP/Hygro]载体是信号通路报告载体；无启动子；含海肾荧光素酶报告基因hRlucP。 |
| 产品目录号: | E6951 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGL4.77[hRlucP/Hygro]载体pGL4.77质粒基本信息
pGL4.77[hRlucP/Hygro]质粒图谱pGL4.77载体图谱和pGL4.77[hRlucP/Hygro]载体序列pGL4.77质粒序列多克隆位点信息
pGL4.77[hRlucP/Hygro]质粒pGL4.77载体简介
The pGL4.77[hRlucP/Hygro] Vector encodes the luciferase reporter gene hRlucP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for hygromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.77[hRlucP/Hygro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The hRlucP reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by hRlucP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.
pGL4.77[hRlucP/Hygro]质粒序列pGL4.77载体序列
LOCUS AY864932 5252 bp DNA circular SYN 31-JAN-2005
DEFINITION Luciferase reporter vector pGL4.77[hRlucP/Hygro], complete
sequence.
ACCESSION AY864932
VERSION AY864932.1 GI:58201881
KEYWORDS .
SOURCE Luciferase reporter vector pGL4.77[hRlucP/Hygro]
ORGANISM Luciferase reporter vector pGL4.77[hRlucP/Hygro]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5252)
AUTHORS Paguio,A., Almond,B. and Browning,J.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5252)
AUTHORS Paguio,A., Almond,B. and Browning,J.
TITLE Direct Submission
JOURNAL Submitted (28-DEC-2004) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..5252
/organism="Luciferase reporter vector
pGL4.77[hRlucP/Hygro]"
/mol_type="genomic DNA"
/db_xref="taxon:309058"
misc_feature 1..70
/note="multiple cloning site"
CDS 100..1158
/note="Renilla luciferase"
/codon_start=1
/transl_table=11
/product="luciferase hRlucP"
/protein_id="AAW66994.1"
/db_xref="GI:58201882"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSHGFPPEVEEQAAGTLPMSCAQ
ESGMDRHPAACASARINV"
misc_feature 1198..1419
/note="SV40 late poly A"
misc_feature 1467..1885
/note="SV40 enhancer/promoter"
CDS 1910..2947
/codon_start=1
/transl_table=11
/product="synthetic hygromycin resistance protein"
/protein_id="AAW66995.1"
/db_xref="GI:58201883"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKEVGRV"
regulatory 2971..3019
/regulatory_class="polyA_signal_sequence"
/note="synthetic polyadenylation signal sequence"
primer_bind 3086..3105
/note="RVprimer4 binding site"
rep_origin 3343
/note="ColEI-derived plasmid replication origin"
CDS complement(4134..4994)
/note="Ampr"
/codon_start=1
/transl_table=11
/product="synthetic beta-lactamase"
/protein_id="AAW66996.1"
/db_xref="GI:58201884"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 5099..5252
/note="synthetic poly(A) signal/transcriptional pause
site"
primer_bind 5201..5220
/note="RVprimer3 binding site"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
1021 aagaacgagc agaattctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc
1081 ctgcccatga gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc
1141 gccaggatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag
1201 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
1261 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
1321 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
1381 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtttg
1441 cgtattgggc gctcttccgc tgatctgcgc agcaccatgg cctgaaataa cctctgaaag
1501 aggaacttgg ttagctacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
1561 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
1621 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
1681 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
1741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
1801 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
1861 aggcctaggc ttttgcaaaa agctcgattc ttctgacact agcgccacca tgaagaagcc
1921 cgaactcacc gctaccagcg ttgaaaaatt tctcatcgag aagttcgaca gtgtgagcga
1981 cctgatgcag ttgtcggagg gcgaagagag ccgagccttc agcttcgatg tcggcggacg
2041 cggctatgta ctgcgggtga atagctgcgc tgatggcttc tacaaagacc gctacgtgta
2101 ccgccacttc gccagcgctg cactacccat ccccgaagtg ttggacatcg gcgagttcag
2161 cgagagcctg acatactgca tcagtagacg cgcccaaggc gttactctcc aagacctccc
2221 cgaaacagag ctgcctgctg tgttacagcc tgtcgccgaa gctatggatg ctattgccgc
2281 cgccgacctc agtcaaacca gcggcttcgg cccattcggg ccccaaggca tcggccagta
2341 cacaacctgg cgggatttca tttgcgccat tgctgatccc catgtctacc actggcagac
2401 cgtgatggac gacaccgtgt ccgccagcgt agctcaagcc ctggacgaac tgatgctgtg
2461 ggccgaagac tgtcccgagg tgcgccacct cgtccatgcc gacttcggca gcaacaacgt
2521 cctgaccgac aacggccgca tcaccgccgt aatcgactgg tccgaagcta tgttcgggga
2581 cagtcagtac gaggtggcca acatcttctt ctggcggccc tggctggctt gcatggagca
2641 gcagactcgc tacttcgagc gccggcatcc cgagctggcc ggcagccctc gtctgcgagc
2701 ctacatgctg cgcatcggcc tggatcagct ctaccagagc ctcgtggacg gcaacttcga
2761 cgatgctgcc tgggctcaag gccgctgcga tgccatcgtc cgcagcgggg ccggcaccgt
2821 cggtcgcaca caaatcgctc gccggagcgc agccgtatgg accgacggct gcgtcgaggt
2881 gctggccgac agcggcaacc gccggcccag tacacgaccg cgcgctaagg aggtaggtcg
2941 agtttaaact ctagaaccgg tcatggccgc aataaaatat ctttattttc attacatctg
3001 tgtgttggtt ttttgtgtgt tcgaactaga tgctgtcgac cgatgccctt gagagccttc
3061 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
3121 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
3181 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
3241 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
3301 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
3361 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
3421 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
3481 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
3541 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
3601 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
3661 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
3721 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
3781 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
3841 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
3901 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
3961 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
4021 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
4081 atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag
4141 tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc
4201 tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca
4261 gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca
4321 gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg
4381 agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg
4441 gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct
4501 ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc
4561 tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta
4621 atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc
4681 ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc
4741 ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc
4801 gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg
4861 atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg
4921 gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa
4981 tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag
5041 tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa
5101 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
5161 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
5221 cagtgcaagt gcaggtgcca gaacatttct ct
//
