| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.78 ; pGL4.78[hRlucCP/Hygro] |
| 质粒类型: | 信号通路报告载体；哺乳动物载体；海肾荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 5303 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | hRluc |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 潮霉素（Hygromycin） |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | HEK293等 |
| 备注: | pGL4.78[hRlucCP/Hygro]载体是信号通路报告载体；无启动子；含海肾荧光素酶报告基因hRlucCP。 |
| 产品目录号: | E6961 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGL4.78[hRlucCP/Hygro]载体pGL4.78质粒基本信息
pGL4.78[hRlucCP/Hygro]质粒图谱pGL4.78载体图谱和pGL4.78[hRlucCP/Hygro]载体序列pGL4.78质粒序列多克隆位点信息
pGL4.78[hRlucCP/Hygro]质粒pGL4.78载体简介
The pGL4.78[hRlucCP/Hygro] Vector encodes the luciferase reporter gene hRlucCP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for hygromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.78[hRlucCP/Hygro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The hRlucCP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by hRlucCP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.
pGL4.78[hRlucCP/Hygro]质粒序列pGL4.78载体序列
LOCUS AY864933 5303 bp DNA circular SYN 31-JAN-2005
DEFINITION Luciferase reporter vector pGL4.78[hRlucCP/Hygro], complete
sequence.
ACCESSION AY864933
VERSION AY864933.1 GI:58201885
KEYWORDS .
SOURCE Luciferase reporter vector pGL4.78[hRlucCP/Hygro]
ORGANISM Luciferase reporter vector pGL4.78[hRlucCP/Hygro]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5303)
AUTHORS Paguio,A., Almond,B. and Browning,J.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5303)
AUTHORS Paguio,A., Almond,B. and Browning,J.
TITLE Direct Submission
JOURNAL Submitted (28-DEC-2004) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..5303
/organism="Luciferase reporter vector
pGL4.78[hRlucCP/Hygro]"
/mol_type="genomic DNA"
/db_xref="taxon:309059"
misc_feature 1..70
/note="multiple cloning site"
CDS 100..1212
/note="Renilla luciferase"
/codon_start=1
/transl_table=11
/product="luciferase hRlucCP"
/protein_id="AAW66997.1"
/db_xref="GI:58201886"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSACKNWFSSLSHFVIHLNSHGF
PPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
misc_feature 1249..1470
/note="SV40 late poly A"
misc_feature 1518..1936
/note="SV40 enhancer/promoter"
CDS 1961..2998
/codon_start=1
/transl_table=11
/product="synthetic hygromycin resistance protein"
/protein_id="AAW66998.1"
/db_xref="GI:58201887"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKEVGRV"
regulatory 3022..3070
/regulatory_class="polyA_signal_sequence"
/note="synthetic polyadenylation signal sequence"
primer_bind 3137..3156
/note="RVprimer4 binding site"
rep_origin 3394
/note="ColEI-derived plasmid replication origin"
CDS complement(4185..5045)
/note="Ampr"
/codon_start=1
/transl_table=11
/product="synthetic beta-lactamase"
/protein_id="AAW66999.1"
/db_xref="GI:58201888"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 5150..5303
/note="synthetic poly(A) signal/transcriptional pause
site"
primer_bind 5252..5271
/note="RVprimer3 binding site"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
1021 aagaacgagc agaattctgc ttgcaagaac tggttcagta gcttaagcca ctttgtgatc
1081 caccttaaca gccacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
1141 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
1201 atcaacgtct aactgcagtc tagagtcggg gcggccggcc gcttcgagca gacatgataa
1261 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
1321 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
1381 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
1441 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgttt gcgtattggg
1501 cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
1561 gttagctacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
1621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
1681 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
1741 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
1801 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
1861 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
1921 cttttgcaaa aagctcgatt cttctgacac tagcgccacc atgaagaagc ccgaactcac
1981 cgctaccagc gttgaaaaat ttctcatcga gaagttcgac agtgtgagcg acctgatgca
2041 gttgtcggag ggcgaagaga gccgagcctt cagcttcgat gtcggcggac gcggctatgt
2101 actgcgggtg aatagctgcg ctgatggctt ctacaaagac cgctacgtgt accgccactt
2161 cgccagcgct gcactaccca tccccgaagt gttggacatc ggcgagttca gcgagagcct
2221 gacatactgc atcagtagac gcgcccaagg cgttactctc caagacctcc ccgaaacaga
2281 gctgcctgct gtgttacagc ctgtcgccga agctatggat gctattgccg ccgccgacct
2341 cagtcaaacc agcggcttcg gcccattcgg gccccaaggc atcggccagt acacaacctg
2401 gcgggatttc atttgcgcca ttgctgatcc ccatgtctac cactggcaga ccgtgatgga
2461 cgacaccgtg tccgccagcg tagctcaagc cctggacgaa ctgatgctgt gggccgaaga
2521 ctgtcccgag gtgcgccacc tcgtccatgc cgacttcggc agcaacaacg tcctgaccga
2581 caacggccgc atcaccgccg taatcgactg gtccgaagct atgttcgggg acagtcagta
2641 cgaggtggcc aacatcttct tctggcggcc ctggctggct tgcatggagc agcagactcg
2701 ctacttcgag cgccggcatc ccgagctggc cggcagccct cgtctgcgag cctacatgct
2761 gcgcatcggc ctggatcagc tctaccagag cctcgtggac ggcaacttcg acgatgctgc
2821 ctgggctcaa ggccgctgcg atgccatcgt ccgcagcggg gccggcaccg tcggtcgcac
2881 acaaatcgct cgccggagcg cagccgtatg gaccgacggc tgcgtcgagg tgctggccga
2941 cagcggcaac cgccggccca gtacacgacc gcgcgctaag gaggtaggtc gagtttaaac
3001 tctagaaccg gtcatggccg caataaaata tctttatttt cattacatct gtgtgttggt
3061 tttttgtgtg ttcgaactag atgctgtcga ccgatgccct tgagagcctt caacccagtc
3121 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
3181 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc
3241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
3301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
3361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
3421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
3481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
3541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
3601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
3661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
3721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
3781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
3841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
3901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
3961 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
4021 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
4081 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
4141 aacttggtct gacagcggcc gcaaatgcta aaccactgca gtggttacca gtgcttgatc
4201 agtgaggcac cgatctcagc gatctgccta tttcgttcgt ccatagtggc ctgactcccc
4261 gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag gccccagcgc agcaatgatg
4321 ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg
4381 gccgagcgaa gaagtggtcc tgctactttg tccgcctcca tccagtctat gagctgctgt
4441 cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc gaagagttgt ggccattgct
4501 actggcatcg tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc tggttcccag
4561 cggtcaagcc gggtcacatg atcacccata ttatgaagaa atgcagtcag ctccttaggg
4621 cctccgatcg ttgtcagaag taagttggcc gcggtgttgt cgctcatggt aatggcagca
4681 ctacacaatt ctcttaccgt catgccatcc gtaagatgct tttccgtgac cggcgagtac
4741 tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa gctgctcttg cccggcgtct
4801 atacgggaca acaccgcgcc acatagcagt actttgaaag tgctcatcat cgggaatcgt
4861 tcttcggggc ggaaagactc aaggatcttg ccgctattga gatccagttc gatatagccc
4921 actcttgcac ccagttgatc ttcagcatct tttactttca ccagcgtttc ggggtgtgca
4981 aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg
5041 ctcatactcg tcctttttca atattattga agcatttatc agggttacta gtacgtctct
5101 caaggataag taagtaatat taaggtacgg gaggtattgg acaggccgca ataaaatatc
5161 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
5221 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
5281 tgcaggtgcc agaacatttc tct
//
