| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pDONR201 |
| 质粒类型: | Gateway载体；克隆载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | -- |
| 克隆方法: | Gateway; BP反应 |
| 载体大小: | 4470 bp |
| 5' 测序引物及序列: | pDONR-F: 5'-TCGCGTTAACGCTAGCATGGATCTC-3' |
| 3' 测序引物及序列: | pDONR-R: 5'-GTAACATCAGAGATTTTGAGACAC-3' |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 / 氯霉素 |
| 筛选标记: | -- |
| 备注: |
载体本身含有CCDb致死基因，能够使得普通的DH5alpha等细菌死亡，必须使用DB3.1感受态细胞进行质粒扩增，用来构建Gateway系统入门载体。 pDONR201尽管含有pUC质粒的起始复制点，但是细菌内仍然是低产量质粒。 构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2 感受态细胞。 |
| 产品目录号: | 11798-014 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
pDONR201载体质粒基本信息
pDONR201质粒图谱载体图谱和pDONR201载体序列质粒序列多克隆位点信息
pDONR201质粒载体简介
pDONR201质粒序列载体序列
LOCUS pDONR201 4470 bp DNA circular SYN
DEFINITION pDONR201
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4470
/organism="pDONR201"
/mol_type="other DNA"
terminator complement(73..100)
/label="rrnB_T2_terminator"
terminator complement(232..275)
/label="rrnB_T1_terminator"
misc_feature complement(331..426)
/label="attL1"
misc_feature 332..563
/label="attP"
misc_feature complement(430..530)
/label="attR2"
misc_feature 430..530
/label="attR1"
misc_feature complement(959..1264)
/label="ccdB"
gene complement(1606..2265)
/label="CAT/CamR"
/gene="CAT/CamR"
CDS complement(1606..2265)
/label="ORF frame 1"
misc_feature complement(2513..2744)
/label="attP"
misc_feature complement(2546..2653)
/label="attR1"
misc_feature 2546..2646
/label="attR2"
misc_feature 2670..2745
/label="attL2"
gene 2868..3677
/label="KanR2"
/gene="KanR2"
CDS 2868..3677
/label="ORF frame 3"
rep_origin 3804..4423
/label="pBR322_origin"
ORIGIN
1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCC AGGAAGAGTT
61 TGTAGAAACG CAAAAAGGCC ATCCGTCAGG ATGGCCTTCT GCTTAGTTTG ATGCCTGGCA
121 GTTTATGGCG GGCGTCCTGC CCGCCACCCT CCGGGCCGTT GCTTCACAAC GTTCAAATCC
181 GCTCCCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG ACAAACAACA GATAAAACGA
241 AAGGCCCAGT CTTCCGACTG AGCCTTTCGT TTTATTTGAT GCCTGGCAGT TCCCTACTCT
301 CGCGTTAACG CTAGCATGGA TCTCGGGCCC CAAATAATGA TTTTATTTTG ACTGATAGTG
361 ACCTGTTCGT TGCAACAAAT TGATGAGCAA TGCTTTTTTA TAATGCCAAG TTTGTACAAA
421 AAAGCAGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT
481 AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG AATCAACTAC
541 TTAGATGGTA TTAGTGACCT GTAGTCGACC GACAGCCTTC CAAATGTTCT TCGGGTGATG
601 CTGCCAACTT AGTCGACCGA CAGCCTTCCA AATGTTCTTC TCAAACGGAA TCGTCGTATC
661 CAGCCTACTC GCTATTGTCC TCAATGCCGT ATTAAATCAT AAAAAGAAAT AAGAAAAAGA
721 GGTGCGAGCC TCTTTTTTGT GTGACAAAAT AAAAACATCT ACCTATTCAT ATACGCTAGT
781 GTCATAGTCC TGAAAATCAT CTGCATCAAG AACAATTTCA CAACTCTTAT ACTTTTCTCT
841 TACAAGTCGT TCGGCTTCAT CTGGATTTTC AGCCTCTATA CTTACTAAAC GTGATAAAGT
901 TTCTGTAATT TCTACTGTAT CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT
961 ATATTCCCCA GAACATCAGG TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT
1021 CAGCCACTTC TTCCCCGATA ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC
1081 GCCAGCTTTC ATCCCCGATA TGCACCACCG GGTAAAGTTC ACGGGAGACT TTATCTGACA
1141 GCAGACGTGC ACTGGCCAGG GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC
1201 TCTGTACATC CACAAACAGA CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT
1261 GCATTTCACC AGTCCCTGTT CTCGTCAGCA AAAGAGCCGT TCATTTCAAT AAACCGGGCG
1321 ACCTCAGCCA TCCCTTCCTG ATTTTCCGCT TTCCAGCGTT CGGCACGCAG ACGACGGGCT
1381 TCATTCTGCA TGGTTGTGCT TACCAGACCG GAGATATTGA CATCATATAT GCCTTGAGCA
1441 ACTGATAGCT GTCGCTGTCA ACTGTCACTG TAATACGCTG CTTCATAGCA CACCTCTTTT
1501 TGACATACTT CGGGTATACA TATCAGTATA TATTCTTATA CCGCAAAAAT CAGCGCGCAA
1561 ATACGCATAC TGTTATCTGG CTTTTAGTAA GCCGGATCCA CGCGATTACG CCCCGCCCTG
1621 CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA
1681 GACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA
1741 TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA
1801 ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT
1861 AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA
1921 CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG
1981 GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC
2041 CATACGGAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA
2101 AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT
2161 CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA
2221 TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG CTTCCTTAGC
2281 TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT CATTATGGTG
2341 AAAGTTGGAA CCTCTTACGT GCCGATCAAC GTCTCATTTT CGCCAAAAGT TGGCCCAGGG
2401 CTTCCCGGTA TCAACAGGGA CACCAGGATT TATTTATTCT GCGAAGTGAT CTTCCGTCAC
2461 AGGTATTTAT TCGGCGCAAA GTGCGTCGGG TGATGCTGCC AACTTAGTCG ACTACAGGTC
2521 ACTAATACCA TCTAAGTAGT TGATTCATAG TGACTGGATA TGTTGTGTTT TACAGTATTA
2581 TGTAGTCTGT TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT CATTTTACGT
2641 TTCTCGTTCA GCTTTCTTGT ACAAAGTGGG CATTATAAGA AAGCATTGCT TATCAATTTG
2701 TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGCAGC
2761 TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAAAT ATATCATCAT
2821 GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC
2881 AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA
2941 AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC
3001 CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG
3061 ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT
3121 TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT
3181 TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT
3241 TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT
3301 TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG
3361 ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC
3421 CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG
3481 ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC
3541 AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC
3601 TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC
3661 TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC
3721 TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA
3781 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
3841 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
3901 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
3961 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
4021 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
4081 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
4141 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
4201 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
4261 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
4321 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
4381 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
4441 GGCCTTTTGC TGGCCTTTTG CTCACATGTT
//
