| 质粒名称: | pIM1773-PRHIII-sfGFP-pNW33N |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 52217 |
| 存储实验室: | Ichiro Matsumura |
|
载体骨架基本信息 |
|
| 载体名称: | pNW33N |
| 载体出品公司: | 芽孢杆菌遗传资源中心 |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 4217 bp |
| 完整质粒大小: | 5062 bp |
| 载体类型: | 细菌表达 |
| 宿生菌： | InvaF' |
| 生长说明： | 这种质粒在大肠杆菌中通常生长缓慢。土芽孢杆菌的生长通常与未转化菌株相当。 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PRHIII-sfGFP |
| 物种来源: | 合成的 |
| 启动子 | PRHIII |
| 克隆方法: | 限制性酶 |
| 5' 克隆位点: | Ecori（未销毁） |
| 3' 克隆位点: | PSTI（未销毁） |
| 5' 测序引物 | N/A |
| 3' 测序引物 | M13-Rev |
|
文献引用方法 |
|
| 材料和方法部分: | pIM1773-PRHIII-sfGFP-pNW33N was a gift from Ichiro Matsumura (Addgene plasmid # 52217 ; http://n2t.net/addgene:52217 ; RRID:Addgene_52217) |
| 参考文献部分: | Transformable facultative thermophile Geobacillus stearothermophilus NUB3621 as a host strain for metabolic engineering. Blanchard K, Robic S, Matsumura I. Appl Microbiol Biotechnol. 2014 May 2. 10.1007/s00253-014-5746-z PubMed 24788326 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pIM1773-PRHIII-sfGFP-pNW33N 5062 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Constitutive sfGFP expression plasmid for use in Geobacillus and E.
coli.
ACCESSION .
VERSION .
KEYWORDS pIM1773-PRHIII-sfGFP-pNW33N
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5062)
AUTHORS Blanchard K, Robic S, Matsumura I
TITLE Transformable facultative thermophile Geobacillus stearothermophilus
NUB3621 as a host strain for metabolic engineering.
JOURNAL Appl Microbiol Biotechnol. 2014 May 2.
PUBMED 24788326
REFERENCE 2 (bases 1 to 5062)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5062
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 869..889
/label=BioBrick suffix
/note="universal suffix for all parts"
primer_bind complement(914..930)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(914..930)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(927..949)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 938..954
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(962..992)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1007..1028
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(1915..2919)
/codon_start=1
/gene="repB"
/product="RepB replication protein"
/label=repB
/note="from Enterococcus faecalis plasmid pAM-alpha-1"
/db_xref="GI:22652809"
/protein_id="AAN03827.1"
/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
primer_bind complement(3440..3457)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3611..4199)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3691..3710)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
misc_feature 5062..21
/label=BioBrick prefix
/note="BioBrick prefix for parts that do not start with
""ATG"""
ORIGIN
1 aattcgcggc cgcttctaga gagcatgctg atttccccca ttctgcaaaa tcctattgtt
61 tatcatacca tgaatgctga tataatgaaa agatacgact gctatgatat gcaaaagaaa
121 aaacaatggt gactaggagt gaatggccat gggctccaaa ggcgaggaat tgtttacggg
181 agttgtcccg attttggtag aattggacgg cgatgttaat ggacataaat tcagcgtgcg
241 cggtgaagga gaaggcgatg ccacgaacgg taaattgacg ctcaaattta tctgtactac
301 ggggaaatta ccagtaccgt ggccgacgct ggtgacaacg ctgacctatg gagtacaatg
361 tttctcacgg tatcccgatc acatgaaacg gcacgacttt ttcaagtcgg cgatgccgga
421 gggctatgtc caagagcgca cgatttcttt taaagacgat ggaacgtata aaacacgtgc
481 cgaagtgaaa tttgagggcg acacgcttgt caacagaatt gagcttaaag gcatcgactt
541 taaagaagat gggaacatac tagggcacaa gttggagtat aacttcaact cgcataacgt
601 ctatataacc gcggacaaac aaaaaaacgg gatcaaagcc aacttcaaaa tccgccataa
661 tgtggaagac ggcagcgtcc aattagcgga tcattaccag caaaatacac cgatcggcga
721 tggacccgta ttactaccgg ataaccacta cttatctaca cagtcggtcc tgtccaaaga
781 tccgaatgaa aaacgcgacc acatggtact gctggaattt gtcaccgcag ccggaatcac
841 ccacggaatg gatgaattat acaagtaata ctagtagcgg ccgctgcagg catgcaagct
901 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
961 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
1021 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
1081 ccttcaaact tcccaaaggc gagccctagt gacattagaa aaccgactgt aaaaagtaca
1141 gtcggcatta tctcatatta taaaagccag tcattaggcc tatctgacaa ttcctgaata
1201 gagttcataa acaatcctgc atgataacca tcacaaacag aatgatgtac ctgtaaagat
1261 agcggtaaat atattgaatt acctttatta atgaattttc ctgctgtaat aatgggtaga
1321 aggtaattac tattattatt gatatttaag ttaaacccag taaatgaagt ccatggaata
1381 atagaaagag aaaaagcatt ttcaggtata ggtgttttgg gaaacaattt ccccgaacca
1441 ttatatttct ctacatcaga aaggtataaa tcataaaact ctttgaagtc attctttaca
1501 ggagtccaaa taccagagaa tgttttagat acaccatcaa aaattgtata aagtggctct
1561 aacttatccc aataacctaa ctctccgtcg ctattgtaac cagttctaaa agctgtattt
1621 gagtttatca cccttgtcac taagaaaata aatgcagggt aaaatttata tccttcttgt
1681 tttatgtttc ggtataaaac actaatatca atttctgtgg ttatactaaa agtcgtttgt
1741 tggttcaaat aatgattaaa tatctctttt ctcttccaat tgtctaaatc aattttatta
1801 aagttcattt gatatgcctc ctaaattttt atctaaagtg aatttaggag gcttacttgt
1861 ctgctttctt cattagaatc aatccttttt taaaagtcaa tcccgtttgt tgaactactc
1921 tttaataaaa taatttttcc gttcccaatt ccacattgca ataatagaaa atccatcttc
1981 atcggctttt tcgtcatcat ctgtatgaat caaatcgcct tcttctgtgt catcaaggtt
2041 taatttttta tgtatttctt ttaacaaacc accataggag attaaccttt tacggtgtaa
2101 accttcctcc aaatcagaca aacgtttcaa attcttttct tcatcatcgg tcataaaatc
2161 cgtatccttt acaggatatt ttgcagtttc gtcaattgcc gattgtatat ccgatttata
2221 tttatttttc ggtcgaatca tttgaacttt tacatttgga tcatagtcta atttcattgc
2281 ctttttccaa aattgaatcc attgtttttg attcacgtag ttttctgtat tcttaaaata
2341 agttggttcc acacatacca atacatgcat gtgctgatta taagaattat ctttattatt
2401 tattgtcact tccgttgcac gcataaaacc aacaagattt ttattaattt ttttatattg
2461 catcattcgg cgaaatcctt gagccatatc tgacaaactc ttatttaatt cttcgccatc
2521 ataaacattt ttaactgtta atgtgagaaa caaccaacga actgttggct tttgtttaat
2581 aacttcagca acaacctttt gtgactgaat gccatgtttc attgctctcc tccagttgca
2641 cattggacaa agcctggatt tacaaaacca cactcgatac aactttcttt cgcctgtttc
2701 acgattttgt ttatactcta atatttcagc acaatctttt actctttcag cctttttaaa
2761 ttcaagaata tgcagaagtt caaagtaatc aacattagcg attttctttt ctctccatgg
2821 tctcactttt ccactttttg tcttgtccac taaaaccctt gatttttcat ctgaataaat
2881 gctactatta ggacacataa tattaaaaga aacccccatc tatttagtta tttgtttggt
2941 cacttataac tttaacagat ggggtttttc tgtgcaacca attttaaggg ttttcaatac
3001 tttaaaacac atacatacca acacttcaac gcacctttca gcaactaaaa taaaaatgac
3061 gttatttcta tatgtatcaa gaatagaaag aactcgtttt tcgctacgct caaaacgcaa
3121 aaaaagcact cattcgagtg ctttttctta tcgctccaaa tcatgcgatt ttttcctctt
3181 tgcttttctt tgctcacgaa gttctcgatc acgctgcaaa acatcttgaa gcgaaaaagt
3241 attcttcttt tcttccgatc gctcatgctg acgcacgaaa agccctctag gcgcatagga
3301 acaactccta aatgcatgtg aggggttttc tcgtccatgt gaacagtcgc atacgcaata
3361 ttttgtttcc catactgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
3421 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
3481 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
3541 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
3601 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
3661 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
3721 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
3781 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
3841 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
3901 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
3961 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
4021 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
4081 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
4141 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
4201 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
4261 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
4321 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
4381 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
4441 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
4501 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccc
4561 gatatgggaa acaaaatatt gcgtatgcga ctgttcacat ggacgagaaa acccctcaca
4621 tgcatttagg agttgttcct atgcgcctag agggcttttc gtgcgtcagc atgagcgatc
4681 ggaagaaaag aagaatactt tttcgcttca agatgttttg cagcgtgatc gagaacttcg
4741 tgagcaaaga aaagcaaaga ggaaaaaatc gcatgatttg gagcgataag aaaaagcact
4801 cgaatgagtg ctttttttgc gttttgagcg tagcgaaaaa cgagttcttt ctattcttga
4861 tacatataga aataacgtca tttttatttt agttgctgaa aggtgcgttg aagtgttggt
4921 atgtatgtga ttcaataatt tcttttactc gctcgttata gtcgatcggt tcatcattca
4981 ccaaatcata attttcatgt gaccgttctt tatcaatatc gggattcgtt ttactttccc
5041 gttctctctg attgtgaaat tg
//
