| 质粒名称: | Flag-SENP1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 17357 |
| 存储实验室: | Edward Yeh |
|
载体骨架基本信息 |
|
| 载体名称: | pFlag-CMV |
| 载体出品公司: | Sigma |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4700 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SENP1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1930 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 标志（主干上的N个终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BglII(未破坏) |
| 3' 克隆位点: | SalI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | Flag-SENP1 was a gift from Edward Yeh (Addgene plasmid # 17357 ; http://n2t.net/addgene:17357 ; RRID:Addgene_17357) |
| 参考文献部分: | SUMO-specific protease 1 is essential for stabilization of HIF1alpha during hypoxia. Cheng J, Kang X, Zhang S, Yeh ET. Cell. 2007 Nov 2. 131(3):584-95. 10.1016/j.cell.2007.08.045 PubMed 17981124 |
买家导航
FlagSENP1载体基本信息
FlagSENP1载体图谱质粒图谱和多克隆位点信息
FlagSENP1载体简介
FlagSENP1载体序列
LOCUS Flag-SENP1 6605 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Flag-SENP1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6605)
AUTHORS Cheng J, Kang X, Zhang S, Yeh ET
TITLE SUMO-specific protease 1 is essential for stabilization of HIF1alpha
during hypoxia.
JOURNAL Cell. 2007 Nov 2. 131(3):584-95.
PUBMED 17981124
REFERENCE 2 (bases 1 to 6605)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6605
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 235..254
/label=T7
/note="T7 promoter, forward primer"
promoter 235..253
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(292..621)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 306..441
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(360..379)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 606..626
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
polyA_signal 650..1272
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind 1054..1073
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
CDS complement(3260..3283)
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
promoter complement(3317..3520)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(3346..3366)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 3521..3900
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind complement(4061..4078)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(4061..4077)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4070..4092)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin complement(4218..4673)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4355..4376)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 4567..4586
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 4700..4804
/gene="bla"
/label=AmpR promoter
CDS 4805..5665
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5023..5042)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5836..6424
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6325..6344
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6578..6595
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttaatac
241 gactcactat agggaattaa ctttctggtt tttcagttcc tcgaggagct ttttgcaaaa
301 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
361 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
421 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
481 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
541 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
601 tccacaccct aactgacaca cattccacag ccaagctggc cgcgtaccca attcaacagg
661 catctactga gtggacccaa cgcatgagag gacagtgcca agcaagcaac tcaaatgtcc
721 caccggttgg gcatggccag gtagcctatg ctgtgtctgg acgtcctcct gctggtatag
781 ttattttaaa atcagaagga cagggaaggg agcagtggtt cacgcctgta atcccagcaa
841 tttgggaggc caaggtgggt agatcacctg agattaggag ttggagacca gcctggccaa
901 tatggtgaaa ccccgtctct accaaaaaaa caaaaattag ctgagcctgg tcatgcatgc
961 ctggaatccc aacaactcgg gaggctgagg caggagaatc gcttgaaccc aggaggcgga
1021 gattgcagtg agccaagatt gtgccactgc actccagctt ggttcccaat agaccccgca
1081 ggccctacag gttgtcttcc caacttgccc cttgctccat accacccccc tccaccccat
1141 aatattatag aaggacacct agtcagacaa aatgatgcaa cttaatttta ttaggacaag
1201 gctggtgggc actggagtgg caacttccag ggccaggaga ggcactgggg aggggtcaca
1261 gggatgccac ccgggatcct ctagagtcga ctcatcacaa gagttttcgg tggaggatct
1321 cccagaccat ccgcttccgg aagtatggca tgtgttgctg tgcgaagttg attggtctgt
1381 ctttggtaat acagtcagca tatttgcagg caaacatccc acagtcactt ccattcatct
1441 gctgaggaat ctcctggctt ttcttgctga aaagctgcca gccattggtg tcaaactctt
1501 tccttttctt gtcaatgctt tcttgcttta ggtattgcaa gagtattctg caggcttcat
1561 tgtttatccc acccatggag tcgtaatagg taatattctt ctttctaaag tccacaacag
1621 ctagacacca gtgtactccc aggtgaatgg gcaccaaaag aatgtcaaca gaaaatacat
1681 ctactttctt tgtccaacgt ttcactgcct gataaccagc cgtttttaat ttagtgaaga
1741 aaaaggtatt aaatgcatgc acacttggca agcccttctc tttacttcgc tccatcagca
1801 tattcatgta gaaattgatg atctcatcat tgagccaatt cagatggttt agagtttgaa
1861 tatctttgcg tgtaatggtc aggcgaaatg cttcactgag aacttcatcc tgattcccat
1921 tacgaaatac attctttatt tctttctcca tttcctctgt aatttcagga aattcatctt
1981 cactatcagt taatttatga cctttttttt gtgtttcttg gacaacagta acaggaatct
2041 ccttttcaag aggtacacga agatgtagtt ctactgaatc atgtactgaa tgttcccgct
2101 cctgcaatct ctggttttga agctgtaagg ccaatgcctt ctgttcttca atctggcgca
2161 atctttctcg tgctcgagaa tcataaacac tagttaattc tttgatccac agctctgcct
2221 ggaagaaagt agaactggga gttggagtct gggaatcttt cactttcagt aaaatcacag
2281 agtctgatcc ttcagattgt gtatttgaag ctgctaagtt atctggctga tgtggaacag
2341 agtggtgatg atggggatga tgaagagtac cagaatcttt ggatccaaat gcaacatctg
2401 gggtataaga agatagggaa tatacactgt gggacagctg ttcctggtta gttaaaatgc
2461 tggcagatcc agatgatgaa gtatcagagc caatgatctg agatgcacaa gagcttccat
2521 ttttaaacag tgagtctttc aaagtatttt tactggaact aagacatcga gacaggtgta
2581 aaggaaaatg tgtggtgggt ttggctatag caaactgttt ccctgtgacc atctgtagca
2641 gctgtctgta aaactctctt tcttcttctt gaactgtctc ttctgctgta ctaacatgtc
2701 gcctctgagt tttcttgggg ctcaaaagac ttcgacgaca tgaaccactc caagatggac
2761 ttggaacagg tttaatagga aaagattttt catatgcaga tacatggcag tgatggtttg
2821 actttcccgc aaaactgttt gataatccac ttgaggtctt tcgggtttcg aggtaaagac
2881 ttcggctgtt tcttgatttt tgtaaagatg agcttgacga tggggtagaa tttctccatt
2941 ggccatttgc actctggcca aaggttctta aatcgcctga gccaagaaaa ctgtctgagg
3001 aaggattatc tgagtaatag cttggattat aagctgcact tcttgtggaa catgtaaaag
3061 atcggtccaa atgtccttgc ctggaagata aaatctgctg gtcagaaagc gaaagctggt
3121 cctctggaaa acctgtttgt ggcaggaggt gggttttgaa tacggagttg tggttcacta
3181 aagtcacttc tccagcatcc atcctcatcc tatcagcaat atcatccata agatctatcg
3241 atgaattcgc ggccgcaagc ttgtcgtcat cgtctttgta gtccatggta gatcaattct
3301 gacggttcac taaacgagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
3361 tttgcgtcaa cggggcgggg ttattacgac attttggaaa gtcccgttga ttttggtgcc
3421 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
3481 gctatccacg cccattggtg tactgccaaa accgcatcac catggtaata gcgatgacta
3541 atacgtagat gtactgccaa gtaggaaagt cccgtaaggt catgtactgg gcataatgcc
3601 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgg acttggcata tgatacactt
3661 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
3721 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
3781 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
3841 ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc
3901 aacatggcgg tcatattgga catgagccaa tataaatgta catattatga tatagataca
3961 acgtatgcaa tggccaatag ccaatattga tttatgctat ataaccaatg actaatatgg
4021 ctaattgcca atattgattc aatgtataga tcagcttggc actggccgtc gttttacaac
4081 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
4141 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
4201 gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
4261 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
4321 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
4381 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
4441 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
4501 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
4561 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
4621 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
4681 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
4741 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
4801 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
4861 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
4921 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
4981 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
5041 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
5101 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
5161 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
5221 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
5281 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
5341 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
5401 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
5461 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
5521 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
5581 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
5641 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
5701 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
5761 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
5821 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
5881 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
5941 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
6001 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
6061 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
6121 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
6181 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
6241 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
6301 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
6361 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
6421 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
6481 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
6541 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
6601 ggaag
//
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