| 质粒名称: | pSLQ1658-dCas9-EGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 51023 |
| 存储实验室: | Bo Huang, Stanley Qi |
|
载体骨架基本信息 |
|
| 载体名称: | pMSCVpuro |
| 载体抗性: | 氨苄霉素 |
| 完整质粒大小: | 11177 bp |
| 载体修饰: | None |
| 载体类型: | 哺乳细胞表达，Retroviral, CRISPR |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dCas9 fuse to EGFP |
| 物种来源: | 人源 |
| 插入大小: | 4887 bp |
| 启动子 | MSCV LTR promoter |
| 标签/融合蛋白: |
EGFP (C terminal on insert)
|
| 克隆方法: | Ligation Independent Cloning |
| 5' 测序引物 | ctcgatcctccctttatccagcc |
| 3' 测序引物 | ggctgctaaagcgcatgct |
|
文献引用方法 |
|
| 材料和方法部分: | pSLQ1658-dCas9-EGFP was a gift from Bo Huang & Stanley Qi (爱迪基因 plasmid # 51023) |
| 参考文献部分: | Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. 10.1016/j.cell.2013.12.001 PubMed 24360272 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSLQ1658-dCas9-EGFP 11177 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging
(the recipient vector can be TetON 3G promoter system).
ACCESSION .
VERSION .
KEYWORDS pSLQ1658-dCas9-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11177)
AUTHORS Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park
J, Blackburn EH, Weissman JS, Qi LS, Huang B
TITLE Dynamic Imaging of Genomic Loci in Living Human Cells by an
Optimized CRISPR/Cas System.
JOURNAL Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
PUBMED 24360272
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pSLQ1658-dCas9-EGFP|
FEATURES Location/Qualifiers
source 1..11177
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 579..920
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 987..1403
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1333..1355
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1371..1387
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
regulatory 1417..1426
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1432..1452
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1453..5553
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5563..5583
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5593..6309
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(5635..5656)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5896..5915)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 6243..6264
/label=EGFP-C
/note="EGFP, forward primer"
promoter 6320..6819
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(6355..6378)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 6752..6771
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 6840..7439
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6840..6859)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 7336..7356
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 7539..8053
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
primer_bind complement(8222..8238)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8222..8238)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8235..8257)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8246..8262
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8270..8300)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8315..8336
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8453..8470)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8624..9212)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8704..8723)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9383..10243)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10006..10025
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10244..10348)
/gene="bla"
/label=AmpR promoter
primer_bind 10416..10434
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(10472..10494)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 10594..10613
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 10807..10829
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 10957..10976
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 11176..515
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
ORIGIN
1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
1381 ccctcactcc ttctctaggc gccggaatta gatctcgcca ccatggctag ccccaaaaag
1441 aagaggaaag tggacaagaa gtattctatc ggactggcca tcgggactaa tagcgtcggg
1501 tgggccgtga tcactgacga gtacaaggtg ccctctaaga agttcaaggt gctcgggaac
1561 accgaccggc attccatcaa gaaaaatctg atcggagctc tcctctttga ttcaggggag
1621 accgctgaag caacccgcct caagcggact gctagacggc ggtacaccag gaggaagaac
1681 cggatttgtt accttcaaga gatattctcc aacgaaatgg caaaggtcga cgacagcttc
1741 ttccataggc tggaagaatc attcctcgtg gaagaggata agaagcatga acggcatccc
1801 atcttcggta atatcgtcga cgaggtggcc tatcacgaga aatacccaac catctaccat
1861 cttcgcaaaa agctggtgga ctcaaccgac aaggcagacc tccggcttat ctacctggcc
1921 ctggcccaca tgatcaagtt cagaggccac ttcctgatcg agggcgacct caatcctgac
1981 aatagcgatg tggataaact gttcatccag ctggtgcaga cttacaacca gctctttgaa
2041 gagaacccca tcaatgcaag cggagtcgat gccaaggcca ttctgtcagc ccggctgtca
2101 aagagccgca gacttgagaa tcttatcgct cagctgccgg gtgaaaagaa aaatggactg
2161 ttcgggaacc tgattgctct ttcacttggg ctgactccca atttcaagtc taatttcgac
2221 ctggcagagg atgccaagct gcaactgtcc aaggacacct atgatgacga tctcgacaac
2281 ctcctggccc agatcggtga ccaatacgcc gaccttttcc ttgctgctaa gaatctttct
2341 gacgccatcc tgctgtctga cattctccgc gtgaacactg aaatcaccaa ggcccctctt
2401 tcagcttcaa tgattaagcg gtatgatgag caccaccagg acctgaccct gcttaaggca
2461 ctcgtccggc agcagcttcc ggagaagtac aaggaaatct tctttgacca gtcaaagaat
2521 ggatacgccg gctacatcga cggaggtgcc tcccaagagg aattttataa gtttatcaaa
2581 cctatccttg agaagatgga cggcaccgaa gagctcctcg tgaaactgaa tcgggaggat
2641 ctgctgcgga agcagcgcac tttcgacaat gggagcattc cccaccagat ccatcttggg
2701 gagcttcacg ccatccttcg gcgccaagag gacttctacc cctttcttaa ggacaacagg
2761 gagaagattg agaaaattct cactttccgc atcccctact acgtgggacc cctcgccaga
2821 ggaaatagcc ggtttgcttg gatgaccaga aagtcagaag aaactatcac tccctggaac
2881 ttcgaagagg tggtggacaa gggagccagc gctcagtcat tcatcgaacg gatgactaac
2941 ttcgataaga acctccccaa tgagaaggtc ctgccgaaac attccctgct ctacgagtac
3001 tttaccgtgt acaacgagct gaccaaggtg aaatatgtca ccgaagggat gaggaagccc
3061 gcattcctgt caggcgaaca aaagaaggca attgtggacc ttctgttcaa gaccaataga
3121 aaggtgaccg tgaagcagct gaaggaggac tatttcaaga aaattgaatg cttcgactct
3181 gtggagatta gcggggtcga agatcggttc aacgcaagcc tgggtaccta ccatgatctg
3241 cttaagatca tcaaggacaa ggattttctg gacaatgagg agaacgagga catccttgag
3301 gacattgtcc tgactctcac tctgttcgag gaccgggaaa tgatcgagga gaggcttaag
3361 acctacgccc atctgttcga cgataaagtg atgaagcaac ttaaacggag aagatatacc
3421 ggatggggac gccttagccg caaactcatc aacggaatcc gggacaaaca gagcggaaag
3481 accattcttg atttccttaa gagcgacgga ttcgctaatc gcaacttcat gcaacttatc
3541 catgatgatt ccctgacctt taaggaggac atccagaagg cccaagtgtc tggacaaggt
3601 gactcactgc acgagcatat cgcaaatctg gctggttcac ccgctattaa gaagggtatt
3661 ctccagaccg tgaaagtcgt ggacgagctg gtcaaggtga tgggtcgcca taaaccagag
3721 aacattgtca tcgagatggc cagggaaaac cagactaccc agaagggaca gaagaacagc
3781 agggagcgga tgaaaagaat tgaggaaggg attaaggagc tcgggtcaca gatccttaaa
3841 gagcacccgg tggaaaacac ccagcttcag aatgagaagc tctatctgta ctaccttcaa
3901 aatggacgcg atatgtatgt ggaccaagag cttgatatca acaggctctc agactacgac
3961 gtggacgcca tcgtccctca gagcttcctc aaagacgact caattgacaa taaggtgctg
4021 actcgctcag acaagaaccg gggaaagtca gataacgtgc cctcagagga agtcgtgaaa
4081 aagatgaaga actattggcg ccagcttctg aacgcaaagc tgatcactca gcggaagttc
4141 gacaatctca ctaaggctga gaggggcgga ctgagcgaac tggacaaagc aggattcatt
4201 aaacggcaac ttgtggagac tcggcagatt actaaacatg tcgcccaaat ccttgactca
4261 cgcatgaata ccaagtacga cgaaaacgac aaacttatcc gcgaggtgaa ggtgattacc
4321 ctgaagtcca agctggtcag cgatttcaga aaggactttc aattctacaa agtgcgggag
4381 atcaataact atcatcatgc tcatgacgca tatctgaatg ccgtggtggg aaccgccctg
4441 atcaagaagt acccaaagct ggaaagcgag ttcgtgtacg gagactacaa ggtctacgac
4501 gtgcgcaaga tgattgccaa atctgagcag gagatcggaa aggccaccgc aaagtacttc
4561 ttctacagca acatcatgaa tttcttcaag accgaaatca cccttgcaaa cggtgagatc
4621 cggaagaggc cgctcatcga gactaatggg gagactggcg aaatcgtgtg ggacaagggc
4681 agagatttcg ctaccgtgcg caaagtgctt tctatgcctc aagtgaacat cgtgaagaaa
4741 accgaggtgc aaaccggagg cttttctaag gaatcaatcc tccccaagcg caactccgac
4801 aagctcattg caaggaagaa ggattgggac cctaagaagt acggcggatt cgattcacca
4861 actgtggctt attctgtcct ggtcgtggct aaggtggaaa aaggaaagtc taagaagctc
4921 aagagcgtga aggaactgct gggtatcacc attatggagc gcagctcctt cgagaagaac
4981 ccaattgact ttctcgaagc caaaggttac aaggaagtca agaaggacct tatcatcaag
5041 ctcccaaagt atagcctgtt cgaactggag aatgggcgga agcggatgct cgcctccgct
5101 ggcgaacttc agaagggtaa tgagctggct ctcccctcca agtacgtgaa tttcctctac
5161 cttgcaagcc attacgagaa gctgaagggg agccccgagg acaacgagca aaagcaactg
5221 tttgtggagc agcataagca ttatctggac gagatcattg agcagatttc cgagttttct
5281 aaacgcgtca ttctcgctga tgccaacctc gataaagtcc ttagcgcata caataagcac
5341 agagacaaac caattcggga gcaggctgag aatatcatcc acctgttcac cctcaccaat
5401 cttggtgccc ctgccgcatt caagtacttc gacaccacca tcgaccggaa acgctatacc
5461 tccaccaaag aagtgctgga cgccaccctc atccaccaga gcatcaccgg actttacgaa
5521 actcggattg acctctcaca gctcggaggg gatgagggag ctcccaagaa aaagcgcaag
5581 gtaggtagtt ccgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
5641 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
5701 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
5761 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
5821 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
5881 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
5941 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
6001 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
6061 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
6121 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
6181 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
6241 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
6301 tacaagtaag aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
6361 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
6421 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
6481 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
6541 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
6601 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
6661 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
6721 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
6781 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
6841 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
6901 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
6961 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
7021 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
7081 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
7141 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
7201 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
7261 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
7321 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
7381 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
7441 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgataa
7501 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
7561 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat
7621 agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata
7681 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
7741 tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
7801 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
7861 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
7921 ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
7981 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
8041 gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg agggtaggag
8101 tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat actccaatac
8161 tcctgaaata gttcattatg gacagcgcag aagagctggg gagaattaat tcgtaatcat
8221 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
8281 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
8341 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
8401 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
8461 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
8521 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
8581 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
8641 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
8701 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
8761 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
8821 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
8881 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
8941 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
9001 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
9061 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
9121 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
9181 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
9241 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
9301 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
9361 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
9421 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
9481 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
9541 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
9601 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
9661 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
9721 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
9781 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
9841 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
9901 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
9961 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
10021 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
10081 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
10141 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
10201 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
10261 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
10321 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
10381 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
10441 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
10501 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
10561 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
10621 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
10681 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
10741 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
10801 ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat gcaaggagat
10861 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
10921 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
10981 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggcgatt
11041 agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata
11101 tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct
11161 ccagaaaaag gggggaa
//
