| 出品公司: | Clontech |
|---|---|
| 载体名称: | RNAi-Ready pSIREN-RetroQ |
| 质粒类型: | 哺乳动物细胞自失活基因沉默逆病毒载体，shRNA |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | Human U6 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6455 bp |
| 5' 测序引物及序列: | U6-F: ATGGACTATCATATGCTTACCGTA (Clontech ) |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 筛选标记: | 嘌呤霉素 (Puromycin) |
| 备注: | |
| 产品目录号: | 631526 |
| 稳定性: | / |
| 组成型: | 诱导型 |
| 病毒/非病毒: | 逆病毒 |
买家导航
RNAi-Ready pSIREN-RetroQ载体质粒基本信息
RNAi-Ready pSIREN-RetroQ质粒图谱载体图谱和RNAi-Ready pSIREN-RetroQ载体序列质粒序列多克隆位点信息
RNAi-Ready pSIREN-RetroQ质粒载体简介
RNAi-Ready pSIREN-RetroQ质粒序列载体序列
LOCUS pSIREN-RetroQ 6455 bp DNA circular 13-NOV-2009
COMMENT Created by Clontech Laboratories Inc.
COMMENT Cat. No. 631526
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
misc_feature 1..727
/label=5'-LTR\(CMV/MSV)
misc_feature 757..1566
/label=Extended\packaging\signal
misc_feature 3161..3586
/label=3'\MMLV\LTR\(deletion\in\U3)
CDS complement(5233..6093)
/label=Amp(R)
misc_feature 3866..4152
/label=SV40\Promoter
promoter 1582..1839
/label=Human\U6\promoter
rep_origin complement(4472..5071)
/label=pUC\origin
promoter 1852..2360
/label=PGK\Promoter
CDS 2381..2980
/label=Puro(R)
rep_origin 4087..4152
/label=SV40\origin
misc_feature 1834..1856
/label=shRNA\Cloning\Site
BASE COUNT 1458 a 1810 c 1668 g 1519 t
ORIGIN
1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
1561 aggcgccgga attgaagatc tgggcaggaa gagggcctat ttcccatgat tccttcatat
1621 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca
1681 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
1741 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
1801 ttcttggctt tatatatctt gtggaaagga cgaggatccg gacaagcttc gaattctacc
1861 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
1921 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
1981 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
2041 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
2101 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
2161 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
2221 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
2281 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
2341 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
2401 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
2461 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
2521 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
2581 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
2641 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
2701 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
2761 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
2821 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
2881 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
2941 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
3001 gcgcccgacc gaaaggagcg cacgacccca tgcatcggcg tctcgagata tcagtggtcc
3061 aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga
3121 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
3181 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
3241 gaatagagaa gttcagatca aggtcaggaa cagatggaac agggtcgacc ctagagaacc
3301 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
3361 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
3421 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
3481 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
3541 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg
3601 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc
3661 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
3721 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
3781 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtagatcc
3841 ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
3901 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
3961 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
4021 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
4081 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
4141 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttact ggcttaacta
4201 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
4261 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
4321 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
4381 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
4441 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
4501 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
4561 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
4621 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
4681 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
4741 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
4801 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
4861 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
4921 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
4981 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
5041 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
5101 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
5161 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
5221 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
5281 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
5341 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
5401 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
5461 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
5521 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
5581 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
5641 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
5701 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
5761 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
5821 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
5881 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
5941 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
6001 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
6061 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
6121 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
6181 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
6241 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
6301 attagcttgg ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc
6361 atgtccaaca ttaccgccat gttgacattg attattgact agttattaat agtaatcaat
6421 tacggggtca ttagttcata gcccatatat ggagt
//
