| 质粒名称: | pDSG320 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 115597 |
| 存储实验室: | Ingmar Riedel-Kruse |
|
载体骨架基本信息 |
|
| 载体名称: | pSB3K3 |
| 完整质粒大小: | 6158 bp |
| 载体类型: | 细菌细胞表达 |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | regulated adhesin construct |
| GenBank编号 | MH492391 |
|
文献引用方法 |
|
| 材料和方法部分: | pDSG320 was a gift from Ingmar Riedel-Kruse (Addgene plasmid # 115597 ; http://n2t.net/addgene:115597 ; RRID:Addgene_115597) |
| 参考文献部分: | A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Glass DS, Riedel-Kruse IH. Cell. 2018 Jul 26;174(3):649-658.e16. doi: 10.1016/j.cell.2018.06.041. Epub 2018 Jul 19. 10.1016/j.cell.2018.06.041 PubMed 30033369 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDSG320 6158 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION surface-displayed Nb2, used in composability experiments; medium
copy.
ACCESSION .
VERSION .
KEYWORDS pDSG320
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6158)
AUTHORS Glass DS, Riedel-Kruse IH
TITLE A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming
Multicellular Morphologies and Patterns.
JOURNAL Cell. 2018 Jul 26;174(3):649-658.e16. doi:
10.1016/j.cell.2018.06.041. Epub 2018 Jul 19.
PUBMED 30033369
REFERENCE 2 (bases 1 to 6158)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6158
/organism="synthetic DNA construct"
/mol_type="other DNA"
RBS 45..56
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 63..686
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
primer_bind complement(136..155)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
terminator 706..777
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 793..820
/label=T7Te terminator
/note="phage T7 early transcription terminator"
protein_bind 835..853
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 860..878
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
RBS 897..908
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
terminator 3289..3360
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 3376..3403
/label=T7Te terminator
/note="phage T7 early transcription terminator"
misc_feature 3410..3430
/label=BioBrick suffix
/note="universal suffix for all parts"
terminator 3431..3488
/label=his operon terminator
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
CDS complement(3646..4461)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 4369..4388
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 4453..4472
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin complement(5056..5601)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(5136..5155)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6069..6087
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
terminator 6091..6134
/label=bacterial terminator
/note="putative bacterial transcription terminator"
misc_feature 6137..6158
/label=BioBrick prefix
/note="BioBrick prefix for parts that do not start with
""ATG"""
ORIGIN
1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc
961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga
1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc
1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc
1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca
1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa
1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg
1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca
1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc
1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg
1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta
1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg
1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta
1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg
1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact
1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt
1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg
1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg
1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg
2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg
2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt
2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg
2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc
2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg
2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg
2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg
2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg
2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg
2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg
2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg
2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg
2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg
2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg
2881 cgactagaca agggcagctt gttgagagcg gggggggaag cgtacaagcc gggggatcgc
2941 tgcgcttgtc gtgtgcagct tccggtattg attccagctc atactgtatg ggttggtttc
3001 gtcagcgtcc gggaaaggag cgcgagggtg tcgcacgtat caacgggttg ggcggcgtta
3061 aaacagctta cgctgactct gttaaagatc gtttcaccat tagtcgtgat aatgcagaaa
3121 atactgttta tttgcaaatg aactccttga aacccgaaga tacagctatt tattattgtg
3181 ctgccaagtt tagtccaggt tattgcggag gatcgtggag caatttcgga tactggggtc
3241 aaggaacgca agtgaccgta tcttcgtaat aatactagag ccaggcatca aataaaacga
3301 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
3361 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagtagcg
3421 gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac
3481 cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg
3541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatctcgagt cccgtcaagt
3601 cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc
3661 gagcatcaaa tgaaactgca atttattcat atcaggatta tcgataccat atttttgaaa
3721 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
3781 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
3841 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
3901 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
3961 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
4021 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
4081 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
4141 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
4201 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
4261 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
4321 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
4381 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctgg agcaagacgt
4441 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
4501 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
4561 aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct
4621 tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac
4681 ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat
4741 tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc gctagcggag
4801 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca
4861 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt
4921 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
4981 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
5041 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
5101 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
5161 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
5221 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
5281 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
5341 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
5401 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
5461 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
5521 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
5581 cgcagaccaa aacgatctca agaagatcat cttattaagg ggtctgacgc tcagtggaac
5641 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
5701 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
5761 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
5821 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
5881 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
5941 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
6001 atccagtcta ttccatggtg ccacctgacg tctaagaaac cattattatc atgacattaa
6061 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
6121 ctaaggatga tttctggaat tcgcggccgc ttctagag
//
