质粒名称: | pDSG318 |
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出品公司: | Addgene |
目录编号: | 115595 |
存储实验室: | Ingmar Riedel-Kruse |
载体骨架基本信息 |
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载体名称: | pSB3K3 |
完整质粒大小: | 5792bp |
载体类型: | 细菌细胞表达 |
载体抗性: | Kanamycin |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 低拷贝 |
插入基因信息 |
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插入基因名称: | regulated adhesin construct |
GenBank编号 | MH492392 |
文献引用方法 |
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材料和方法部分: | pDSG318 was a gift from Ingmar Riedel-Kruse (Addgene plasmid # 115595 ; http://n2t.net/addgene:115595 ; RRID:Addgene_115595) |
参考文献部分: | A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Glass DS, Riedel-Kruse IH. Cell. 2018 Jul 26;174(3):649-658.e16. doi: 10.1016/j.cell.2018.06.041. Epub 2018 Jul 19. 10.1016/j.cell.2018.06.041 PubMed 30033369 |
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LOCUS pDSG318 5792 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION surface-displayed Ag2, used in composability experiments; medium copy. ACCESSION . VERSION . KEYWORDS pDSG318 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5792) AUTHORS Glass DS, Riedel-Kruse IH TITLE A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. JOURNAL Cell. 2018 Jul 26;174(3):649-658.e16. doi: 10.1016/j.cell.2018.06.041. Epub 2018 Jul 19. PUBMED 30033369 REFERENCE 2 (bases 1 to 5792) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..5792 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 45..56 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 63..686 /codon_start=1 /gene="tetR from transposon Tn10" /product="tetracycline repressor TetR" /label=TetR /note="TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline." /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS" primer_bind complement(136..155) /label=Tet-R /note="Tetracycline resistance gene, reverse primer" terminator 706..777 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 793..820 /label=T7Te terminator /note="phage T7 early transcription terminator" protein_bind 835..853 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 860..878 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" RBS 897..908 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" terminator 2923..2994 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 3010..3037 /label=T7Te terminator /note="phage T7 early transcription terminator" misc_feature 3044..3064 /label=BioBrick suffix /note="universal suffix for all parts" terminator 3065..3122 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." CDS complement(3280..4095) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 4003..4022 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 4087..4106 /label=pENTR-R /note="pENTR vectors, reverse primer" rep_origin complement(4690..5235) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind complement(4770..4789) /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5703..5721 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 5725..5768 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 5771..5792 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact 61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg 121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta 181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt 241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac 301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac 361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt 421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt 481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa 541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc 601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac 661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag 721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta 841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag 901 aggagaaata ctagatgatt actcatggtt gttatacccg gacccggcac aagcataagc 961 taaaaaaaac attgattatg cttagtgctg gtttaggatt gtttttttat gttaatcaga 1021 actcatttgc aaatggtgaa aattatttta aattgggttc ggattcaaaa ctgttaactc 1081 atgatagcta tcagaatcgc cttttttata cgttgaaaac tggtgaaact gttgccgatc 1141 tttctaaatc gcaagatatt aatttatcga cgatttggtc gttgaataag catttataca 1201 gttctgaaag cgaaatgatg aaggccgcgc ctggtcagca gatcattttg ccactcaaaa 1261 aacttccctt tgaatacagt gcactaccac ttttaggttc ggcacctctt gttgctgcgg 1321 gtggtgttgc tggtcacacg aataaactga ctaaaatgtc cccggacgtg accaaaagca 1381 acatgaccga tgacaaggca ttaaattatg cggcacaaca ggcggcgagt ctcggtagcc 1441 agcttcagtc gcgatctctg aacggcgatt acgcgaaaga taccgctctt ggtatcgctg 1501 gtaaccaggc ttcgtcacag ttgcaggcct ggttacaaca ttatggaacg gcagaggtta 1561 atctgcaaag tggtaataac tttgacggta gttcactgga cttcttatta ccgttctatg 1621 attccgaaaa aatgctggca tttggtcagg tcggagcgcg ttacattgac tcccgcttta 1681 cggcaaattt aggtgcgggt cagcgttttt tccttcctgc aaacatgttg ggctataacg 1741 tcttcattga tcaggatttt tctggtgata atacccgttt aggtattggt ggcgaatact 1801 ggcgagacta tttcaaaagt agcgttaacg gctatttccg catgagcggc tggcatgagt 1861 catacaataa gaaagactat gatgagcgcc cagcaaatgg cttcgatatc cgttttaatg 1921 gctatctacc gtcatatccg gcattaggcg ccaagctgat atatgagcag tattatggtg 1981 ataatgttgc tttgtttaat tctgataagc tgcaatcgaa tcctggtgcg gcgaccgttg 2041 gtgtaaacta tactccgatt cctctggtga cgatggggat cgattaccgt catggtacgg 2101 gtaatgaaaa tgatctcctt tactcaatgc agttccgtta tcagtttgat aaatcgtggt 2161 ctcagcaaat tgaaccacag tatgttaacg agttaagaac attatcaggc agccgttacg 2221 atctggttca gcgtaataac aatattattc tggagtacaa gaagcaggat attctttctc 2281 tgaatattcc gcatgatatt aatggtactg aacacagtac gcagaagatt cagttgatcg 2341 ttaagagcaa atacggtctg gatcgtatcg tctgggatga tagtgcatta cgcagtcagg 2401 gcggtcagat tcagcatagc ggaagccaaa gcgcacaaga ctaccaggct attttgcctg 2461 cttatgtgca aggtggcagc aatatttata aagtgacggc tcgcgcctat gaccgtaatg 2521 gcaatagctc taacaatgta cagcttacta ttaccgttct gtcgaatggt caagttgtcg 2581 accaggttgg ggtaacggac tttacggcgg ataagacttc ggctaaagcg gataacgccg 2641 ataccattac ttataccgcg acggtgaaaa agaatggggt agctcaggct aatgtccctg 2701 tttcatttaa tattgtttca ggaactgcaa ctcttggggc aaatagtgcc aaaacggatg 2761 ctaacggtaa ggcaaccgta acgttgaagt cgagtacgcc aggacaggtc gtcgtgtctg 2821 ctaaaaccgc ggagatgact tcagcactta atgccagtgc ggttatattt tttgatggtg 2881 cgactagaga accagaggct taataatact agagccaggc atcaaataaa acgaaaggct 2941 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 3001 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactagt agcggccgct 3061 gcagtccggc aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa 3121 gattacttcg cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3181 tgcggcgagc ggtatcagct cactcaaagg cggtaatctc gagtcccgtc aagtcagcgt 3241 aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact catcgagcat 3301 caaatgaaac tgcaatttat tcatatcagg attatcgata ccatattttt gaaaaagccg 3361 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta 3421 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa 3481 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa 3541 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa 3601 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac 3661 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac 3721 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc 3781 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg 3841 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt 3901 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt 3961 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata 4021 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg 4081 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt 4141 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg 4201 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga 4261 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa 4321 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc 4381 ctggtatgag tcagcaacac cttcttcacg aggcagacct cagcgctagc ggagtgtata 4441 ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa 4501 aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc 4561 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg 4621 gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc 4681 aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa 4741 tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc 4801 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc 4861 gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg 4921 actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc 4981 ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat 5041 ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt 5101 ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc 5161 ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga 5221 ccaaaacgat ctcaagaaga tcatcttatt aaggggtctg acgctcagtg gaacgaaaac 5281 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 5341 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 5401 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 5461 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 5521 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 5581 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 5641 tctattccat ggtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 5701 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg 5761 atgatttctg gaattcgcgg ccgcttctag ag //
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