LOCUS VEGFR2-mCh 8856 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Encodes for human VEGFR2 fluorescently labeled with mCherry on the
C-Terminus via a 3 amino acid (GGS) flexible linker.
ACCESSION .
VERSION .
KEYWORDS VEGFR2-mCh
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8856)
AUTHORS Sarabipour S, Ballmer-Hofer K, Hristova K
TITLE VEGFR-2 conformational switch in response to ligand binding.
JOURNAL Elife. 2016 Apr 7;5:e13876. doi: 10.7554/eLife.13876.
PUBMED 27052508
REFERENCE 2 (bases 1 to 8856)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8856
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 45..633
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 534..553
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
enhancer 808..1111
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1112..1315
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1266..1286
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 1531..1557
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 5575..6279
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind complement(5717..5735)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 5985..6004
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 6391..6512
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6428..6447)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6482..6501
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(6519..6974)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6656..6677)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6868..6887
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 7001..7105
/gene="bla"
/label=AmpR promoter
promoter 7107..7464
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(7130..7150)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 7315..7450
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7377..7396
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 7499..8293
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7553..7572)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 8163..8182
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(8481..8500)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 8525..8572
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
1 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
61 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
181 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
661 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
721 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
781 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
841 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
901 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
961 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
1021 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
1081 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
1141 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
1201 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
1261 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
1321 agtgaaccgt cagatccgct agagagctta tcgattctag ccgagtcccc gcctcgccgc
1381 caacgccaca accaccgcgc acggccccct gactccgtcc agtattgatc gggagagccg
1441 gagcgagctc ttcggggagc agcgatggag agcaaggtgc tgctggccgt cgccctgtgg
1501 ctctgcgtgg agacccgggc cgcctctgtg tacccatacg atgttccaga ttacgctggt
1561 ttgcctagtg tttctcttga tctgcccagg ctcagcatac aaaaagacat acttacaatt
1621 aaggctaata caactcttca aattacttgc aggggacaga gggacttgga ctggctttgg
1681 cccaataatc agagtggcag tgagcaaagg gtggaggtga ctgagtgcag cgatggcctc
1741 ttctgtaaga cactcacaat tccaaaagtg atcggaaatg acactggagc ctacaagtgc
1801 ttctaccggg aaactgactt ggcctcggtc atttatgtct atgttcaaga ttacagatct
1861 ccatttattg cttctgttag tgaccaacat ggagtcgtgt acattactga gaacaaaaac
1921 aaaactgtgg tgattccatg tctcgggtcc atttcaaatc tcaacgtgtc actttgtgca
1981 agatacccag aaaagagatt tgttcctgat ggtaacagaa tttcctggga cagcaagaag
2041 ggctttacta ttcccagcta catgatcagc tatgctggca tggtcttctg tgaagcaaaa
2101 attaatgatg aaagttacca gtctattatg tacatagttg tcgttgtagg gtataggatt
2161 tatgatgtgg ttctgagtcc gtctcatgga attgaactat ctgttggaga aaagcttgtc
2221 ttaaattgta cagcaagaac tgaactaaat gtggggattg acttcaactg ggaataccct
2281 tcttcgaagc atcagcataa gaaacttgta aaccgagacc taaaaaccca gtctgggagt
2341 gagatgaaga aatttttgag caccttaact atagatggtg taacccggag tgaccaagga
2401 ttgtacacct gtgcagcatc cagtgggctg atgaccaaga agaacagcac atttgtcagg
2461 gtccatgaaa aaccttttgt tgcttttgga agtggcatgg aatctctggt ggaagccacg
2521 gtgggggagc gtgtcagaat ccctgcgaag taccttggtt acccaccccc agaaataaaa
2581 tggtataaaa atggaatacc ccttgagtcc aatcacacaa ttaaagcggg gcatgtactg
2641 acgattatgg aagtgagtga aagagacaca ggaaattaca ctgtcatcct taccaatccc
2701 atttcaaagg agaagcagag ccatgtggtc tctctggttg tgtatgtccc accccagatt
2761 ggtgagaaat ctctaatctc tcctgtggat tcctaccagt acggcaccac tcaaacgctg
2821 acatgtacgg tctatgccat tcctcccccg catcacatcc actggtattg gcagttggag
2881 gaagagtgcg ccaacgagcc cagccaagct gtctcagtga caaacccata cccttgtgaa
2941 gaatggagaa gtgtggagga cttccaggga ggaaataaaa ttgaagttaa taaaaatcaa
3001 tttgctctaa ttgaaggaaa aaacaaaact gtaagtaccc ttgttatcca agcggcaaat
3061 gtgtcagctt tgtacaaatg tgaagcggtc aacaaagtcg ggagaggaga gagggtgatc
3121 tccttccacg tgaccagggg tcctgaaatt actttgcaac ctgacatgca gcccactgag
3181 caggagagcg tgtctttgtg gtgcactgca gacagatcta cgtttgagaa cctcacatgg
3241 tacaagcttg gcccacagcc tctgccaatc catgtgggag agttgcccac acctgtttgc
3301 aagaacttgg atactctttg gaaattgaat gccaccatgt tctctaatag cacaaatgac
3361 attttgatca tggagcttaa gaatgcatcc ttgcaggacc aaggagacta tgtctgcctt
3421 gctcaagaca ggaagaccaa gaaaagacat tgcgtggtca ggcagctcac agtcctagag
3481 cgtgtggcac ccacgatcac aggaaacctg gagaatcaga cgacaagtat tggggaaagc
3541 atcgaagtct catgcacggc atctgggaat ccccctccac agatcatgtg gtttaaagat
3601 aatgagaccc ttgtagaaga ctcaggcatt gtattgaagg atgggaaccg gaacctcact
3661 atccgcagag tgaggaagga ggacgaaggc ctctacacct gccaggcatg cagtgttctt
3721 ggctgtgcaa aagtggaggc atttttcata atagaaggtg cccaggaaaa gacgaacttg
3781 gaaatcatta ttctagtagg cacggcggtg attgccatgt tcttctggct acttcttgtc
3841 atcatcctac ggaccgttaa gcgggccaat ggaggggaac tgaagacagg ctacttgtcc
3901 atcgtcatgg atccagatga actcccattg gatgaacatt gtgaacgact gccttatgat
3961 gccagcaaat gggaattccc cagagaccgg ctgaagctag gtaagcctct tggccgtggt
4021 gcctttggcc aagtgattga agcagatgcc tttggaattg acaagacagc aacttgcagg
4081 acagtagcag tcaaaatgtt gaaagaagga gcaacacaca gtgagcatcg agctctcatg
4141 tctgaactca agatcctcat tcatattggt caccatctca atgtggtcaa ccttctaggt
4201 gcctgtacca agccaggagg gccactcatg gtgattgtgg aattctgcaa atttggaaac
4261 ctgtccactt acctgaggag caagagaaat gaatttgtcc cctacaagac caaaggggca
4321 cgattccgtc aagggaaaga ctacgttgga gcaatccctg tggatctgaa acggcgcttg
4381 gacagcatca ccagtagcca gagctcagcc agctctggat ttgtggagga gaagtccctc
4441 agtgatgtag aagaagagga agctcctgaa gatctgtata aggacttcct gaccttggag
4501 catctcatct gttacagctt ccaagtggct aagggcatgg agttcttggc atcgcgaaag
4561 tgtatccaca gggacctggc ggcacgaaat atcctcttat cggagaagaa cgtggttaaa
4621 atctgtgact ttggcttggc ccgggatatt tataaagatc cagattatgt cagaaaagga
4681 gatgctcgcc tccctttgaa atggatggcc ccagaaacaa tttttgacag agtgtacaca
4741 atccagagtg acgtctggtc ttttggtgtt ttgctgtggg aaatattttc cttaggtgct
4801 tctccatatc ctggggtaaa gattgatgaa gaattttgta ggcgattgaa agaaggaact
4861 agaatgaggg cccctgatta tactacacca gaaatgtacc agaccatgct ggactgctgg
4921 cacggggagc ccagtcagag acccacgttt tcagagttgg tggaacattt gggaaatctc
4981 ttgcaagcta atgctcagca ggatggcaaa gactacattg ttcttccgat atcagagact
5041 ttgagcatgg aagaggattc tggactctct ctgcctacct cacctgtttc ctgtatggag
5101 gaggaggaag tatgtgaccc caaattccat tatgacaaca cagcaggaat cagtcagtat
5161 ctgcagaaca gtaagcgaaa gagccggcct gtgagtgtaa aaacatttga agatatcccg
5221 ttagaagaac cagaagtaaa agtaatccca gatgacaacc agacggacag tggtatggtt
5281 cttgcctcag aagagctgaa aactttggaa gacagaacca aattatctcc atcttttggt
5341 ggaatggtgc ccagcaaaag cagggagtct gtggcatctg aaggctcaaa ccagacaagc
5401 ggctaccagt ccggatatca ctccgatgac acagacacca ccgtgtactc cagtgaggaa
5461 gcagaacttt taaagctgat agagattgga gtgcaaaccg gtagcacagc ccagattctc
5521 cagcctgact cggggaccac actgagctct cctcctgttc gtacgggtgg ttcagtgagc
5581 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
5641 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
5701 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
5761 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
5821 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
5881 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
5941 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
6001 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
6061 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
6121 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
6181 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
6241 ggccgccact ccaccggcgg catggacgag ctgtacaaga cgcgtactca tctcagaaga
6301 ggatctgtga taaaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
6361 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
6421 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
6481 tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt
6541 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
6601 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
6661 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
6721 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
6781 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
6841 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
6901 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
6961 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
7021 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
7081 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
7141 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
7201 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
7261 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
7321 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
7381 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
7441 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
7501 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
7561 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
7621 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
7681 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
7741 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
7801 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
7861 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
7921 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
7981 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
8041 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
8101 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
8161 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
8221 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
8281 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
8341 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
8401 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
8461 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
8521 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
8581 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
8641 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
8701 ctcgcagcca acgtcggggc ggcaggccct gtcatagcct caggttactc atatatactt
8761 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
8821 aatctcatga ccaaaatccc ttaacgtgag ttttcg
//
