| 质粒名称: | pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141380 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | 8825bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-nsp14 |
| 物种来源: | SARS-CoV-2 |
| 基因突变: | human codon optimized |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (N terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: |
pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141380 ; http://n2t.net/addgene:141380 ; RRID:Addgene_141380)
|
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro 10491 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10491)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 10491)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10491
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2338..3519
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2568..3510
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3467..3487
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 3553..3576
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 3613..3636
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 5241..5814
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(5408..5425)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 5635..5654
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 5847..6446
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5847..5866)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6343..6363
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 6460..7048
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6513..6533)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6931..6942)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 7255..7888
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(8016..8032)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8016..8032)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8029..8051)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8040..8056
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8064..8094)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8109..8130
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8247..8264)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8418..9006)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8498..8517)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9177..10037)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9800..9819
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10038..10142)
/gene="bla"
/label=AmpR promoter
polyA_signal 10190..10324
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10227..10246)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10281..10300
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
3541 gccgccacca tgtggtccca tccgcagttt gagaagggtg gtggttcagg cggaggctcc
3601 gggggcggga gctggtctca cccgcaattt gaaaaaggcg ctgcggctgc tgaaaatgta
3661 acgggcttgt ttaaagactg tagtaaagtg atcacaggac tccaccccac acaagcacct
3721 acccaccttt ccgtagatac gaagttcaaa acggaaggat tgtgtgtgga tataccaggg
3781 ataccaaagg atatgacgta ccgaaggctg atttccatga tgggttttaa gatgaattac
3841 caagttaatg gctaccccaa catgttcatc accagggagg aggcaattag acacgtaaga
3901 gcctggatag gcttcgatgt tgaaggttgc catgcaaccc gggaagccgt aggcacaaac
3961 cttccgttgc agcttggctt ttccacgggc gtcaacctcg ttgccgtacc gactggctat
4021 gttgacacgc cgaacaacac cgatttctct cgcgtaagtg ctaagcctcc tccgggagat
4081 caattcaagc atcttatacc tctcatgtac aagggactgc cgtggaatgt agttcgcatt
4141 aagatcgtgc agatgctctc cgatactttg aaaaatctga gcgacagggt ggtcttcgtt
4201 ctgtgggcac atggcttcga acttacttct atgaaatact ttgtaaaaat cgggcccgag
4261 cggacatgtt gtttgtgcga ccgacgagct acttgtttta gcacagcatc tgacacctac
4321 gcatgctggc accacagtat aggcttcgat tacgtctaca atccctttat gatagacgta
4381 caacaatggg gctttacggg taacttgcag agtaatcacg atctttactg ccaagttcat
4441 gggaacgcac acgtggcctc ctgcgatgcg ataatgacga ggtgcttggc cgtgcacgag
4501 tgctttgtta agcgggtcga ttggactata gagtatccca taatcggtga cgaacttaaa
4561 attaatgctg catgcaggaa agtgcagcac atggtagtaa aagcagcgct cctcgcggat
4621 aagtttcctg tgctccacga tatcggcaac cctaaggcca ttaaatgcgt cccccaagct
4681 gatgtagagt ggaaattcta tgacgctcag ccatgtagtg acaaagcata caagattgaa
4741 gaattgtttt attcatatgc gacacactcc gataagttca ctgacggagt ttgtctcttt
4801 tggaactgta atgttgatag gtatccggcg aatagcatag tgtgtcgatt tgacacgcgc
4861 gttctctcta atttgaacct ccctggctgc gacggggggt ctttgtacgt aaacaagcat
4921 gcatttcata ccccagcttt tgacaaatcc gctttcgtaa atctgaaaca acttcccttc
4981 ttctattatt cagatagccc ttgcgaatct cacggtaagc aagtggttag tgacattgac
5041 tatgtaccac ttaaaagtgc aacgtgcatt acacgatgca atttgggggg cgcggtttgc
5101 cgacaccatg ccaacgaata cagattgtac ctggatgcat ataacatgat gataagtgca
5161 gggtttagtc tttgggtgta taagcagttc gatacgtata atctctggaa cacatttacc
5221 cgattgcagt agggatcccg cccctctccc tccccccccc ctaacgttac tggccgaagc
5281 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
5341 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
5401 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
5461 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
5521 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
5581 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
5641 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
5701 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
5761 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc
5821 cacaacccac aaggagacga ccttccatga ccgagtacaa gcccacggtg cgcctcgcca
5881 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
5941 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
6001 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
6061 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
6121 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
6181 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
6241 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
6301 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
6361 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
6421 gcatgacccg caagcccggt gcctagacgc gtctggaaca atcaacctct ggattacaaa
6481 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
6541 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
6601 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
6661 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
6721 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
6781 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
6841 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
6901 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
6961 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
7021 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
7081 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
7141 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
7201 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
7261 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
7321 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
7381 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
7441 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
7501 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
7561 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
7621 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
7681 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
7741 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
7801 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
7861 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
7921 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgttta
7981 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
8041 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
8101 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
8161 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
8221 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
8281 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
8341 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
8401 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
8461 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
8521 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
8581 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
8641 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
8701 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
8761 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
8821 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
8881 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
8941 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
9001 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
9061 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
9121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
9181 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
9241 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
9301 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
9361 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
9421 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
9481 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
9541 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
9601 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
9661 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
9721 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
9781 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
9841 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
9901 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
9961 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
10021 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
10081 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
10141 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat
10201 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
10261 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
10321 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
10381 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
10441 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
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