pAAV.CMV.LacZ.bGH

LOCUS       pAAV.CMV.LacZ.bGH         7152 bp ds-DNA     circular SYN 07-AUG-2018
DEFINITION  AAV expression of LacZ from CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV.CMV.LacZ.bGH
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7152)
  TITLE     AAV plasmids
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        31..61
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    69..85
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     74..96
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     93..109
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     93..109
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   147..276
                     /label=AAV2 ITR
     enhancer        417..720
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        721..924
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     875..895
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             965..4039
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(985..1001)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4047..4064)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4053..4260
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4348..4477
                     /label=AAV2 ITR
     primer_bind     complement(4496..4512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4654..5109
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4741..4760)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4951..4972
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5135..5239
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5240..6100
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5458..5477)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6271..6859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6760..6779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7013..7030
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7147..16
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
       61 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      121 cagatttaat taaggcctta attaggctgc gcgctcgctc gctcactgag gccgcccggg
      181 caaagcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca
      241 gagagggagt ggccaactcc atcactaggg gttccttgta gttaatgatt aacccgccat
      301 gctacttatc tacgtagcca tgctctagga agatcggaat tcgcccttaa gctagctagt
      361 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      421 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      481 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      541 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      601 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      661 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      721 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      781 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      841 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      901 tgggaggtct atataagcag agctggttta gtgaaccgtc agatcctgca gataagcggc
      961 cgccatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     1021 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     1081 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1141 gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct
     1201 tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc
     1261 catctacacc aacgtgacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa
     1321 tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca
     1381 gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg
     1441 ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg
     1501 cgccggagaa aaccgcctcg cggtgatggt gctgcgctgg agtgacggca gttatctgga
     1561 agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc
     1621 gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc
     1681 tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt
     1741 ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat
     1801 tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc
     1861 gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc
     1921 cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga
     1981 aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga
     2041 gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct
     2101 gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg
     2161 gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca
     2221 cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga
     2281 acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct
     2341 ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt
     2401 cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat
     2461 tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg
     2521 gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata
     2581 cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca
     2641 gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata
     2701 tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga
     2761 tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac
     2821 ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt
     2881 gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct
     2941 ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca
     3001 gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt
     3061 acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca
     3121 gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc
     3181 gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt
     3241 taaccgccag tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac
     3301 gccgctgcgc gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc
     3361 gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc
     3421 cgaagcagcg ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac
     3481 cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat
     3541 tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca
     3601 tccggcgcgg attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct
     3661 cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg
     3721 ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg
     3781 ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa
     3841 catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc
     3901 ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc
     3961 ctggagcccg tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc attaccagtt
     4021 ggtctggtgt caaaaataag gatctgcctc gactgtgcct tctagttgcc agccatctgt
     4081 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4141 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4201 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4261 ctcgagttaa gggcgaattc ccgataagga tcttcctaga gcatggctac gtagataagt
     4321 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct
     4381 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     4441 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagcct taattaacct aattcactgg
     4501 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     4561 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4621 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     4681 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4741 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4801 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4861 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4921 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4981 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5041 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     5101 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5161 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5221 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5281 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5341 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5401 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5461 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5521 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5581 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5641 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5701 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5761 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5821 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5881 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5941 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6001 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6061 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6121 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6181 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6241 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6301 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6361 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6421 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6481 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6541 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6601 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6661 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6721 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6781 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6841 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6901 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6961 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7021 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     7081 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7141 cgcaattaat gt
//