质粒名称: | pCDEF3-muSOX |
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出品公司: | 爱迪基因 |
目录编号: | 131695 |
存储实验室: | Britt Glaunsinger |
载体骨架基本信息 |
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载体名称: | pCDEF3 |
载体抗性: | 氨苄青霉素 |
载体类型: | 哺乳细胞表达 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | muSOX,mORF37 |
物种来源: | MHV68 |
插入大小: | 1461 bp |
启动子 | CMV |
5' 测序引物 | CGCAAATGGGCGGTAGGCGTG |
3' 测序引物 | gcgatgcaatttcctcattt |
文献引用方法 |
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材料和方法部分: | pCDEF3-muSOX was a gift from Britt Glaunsinger (Addgene plasmid # 131695 ; http://n2t.net/addgene:131695 ; RRID:Addgene_131695) |
买家导航
pCDEF3muSOX载体基本信息
pCDEF3muSOX载体图谱质粒图谱和多克隆位点信息
pCDEF3muSOX载体简介
pCDEF3muSOX载体序列
LOCUS pCDEF3-muSOX 6571 bp ds-DNA circular SYN 20-SEP-2019 DEFINITION expresses muSOX in mammalian cells. ACCESSION . VERSION . KEYWORDS pCDEF3-muSOX SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6571) TITLE Xrn1 activity broadly represses RNA polymerase II occupancy at mammalian but not viral promoters during herpesvirus infection JOURNAL Unpublished REFERENCE 2 (bases 1 to 6571) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 20, 2019 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6571 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(59..647) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(139..158) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(818..1678) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1441..1460 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1679..1783) /gene="bla" /label=AmpR promoter primer_bind complement(1858..1877) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 2049..2428 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2429..2632 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2583..2603 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 2634..2652 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2655..2673 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 2683..2707 /label=LNCX /note="Human CMV promoter, forward primer" CDS 2805..2828 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" CDS 2865..2888 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" primer_bind complement(4393..4410) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4399..4623 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4669..5097 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4756..4775) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4966..4987 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5106..5126) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5111..5440 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5291..5426 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5353..5372 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 5488..5535 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 5554..5928 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 6058..6179 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6095..6114) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6149..6168 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6228..6244) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6228..6244) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6241..6263) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6252..6268 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6276..6306) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6321..6342 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6459..6476) /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 61 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 121 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 181 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 241 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 301 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 361 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 421 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 481 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 541 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 601 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 661 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 721 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 781 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 841 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 901 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 961 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 1021 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 1081 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 1141 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 1201 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 1261 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 1321 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 1381 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 1441 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 1501 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 1561 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 1621 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 1681 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 1741 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 1801 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac 1861 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt 1921 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg 1981 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata 2041 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 2101 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 2161 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 2221 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 2281 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 2341 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 2401 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 2461 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 2521 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 2581 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat 2641 cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg 2701 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg 2761 atccagcctc cggactctag cgtttaaact taagcttacc aatgtggagc cacccccagt 2821 tcgagaaggg cggcggcagc ggcggcggca gcggcggcgg cagctggagc cacccccagt 2881 tcgagaaggg ggcggccgca atggaagggt cgattattct ggattttttt gaaaaggatg 2941 atttgttgga agttctggga aacatggccc gggaaaatca actagaatca ctggcaactc 3001 ttaatttttc tgcatttata agatcacccc aagttcaaga gctcctggca acatcaaaga 3061 cttctgtacg tatttctgat atgaggttga cgtactatta ctttctgttt ctcagactta 3121 atgaatatat tgggaatact gccataatgg gcgtttttaa ggacatgatg cacctgacag 3181 acaacagtga ggtgtctgcc gtgtatgctg cgtgtcggga cgtgacccca gatataaagt 3241 atgcagtgtg ccagagaata gaagccctga ctaggggaca gcaggataat gaactatggg 3301 acattctgcg tgacggcatg atctcatcat ctaaatttca gtgggctgta aaacaacata 3361 gcatcaacaa gaaactgttt aatccacagc ctataaaggt taatcactat tttgctggtc 3421 ctctagcctt tgggataaga tgtgaacaaa ctgttaaaaa aattttgagt gagcttattc 3481 atccaaactt gccaagattt catgattgtg gatttttgcc cagtgcaatt gatggaattt 3541 ttggagtttc cctggacact gctttcaatg tgtttacaga tagcagcgga cttgtgcact 3601 ttgaaccaga tagtattgtg tatgaaataa agtccagata taagtaccag ttttcaaaat 3661 ctgaatttga tggtctggcc aagaaatatt tggatctcta caaaaacccc tgtgaggcaa 3721 cttttatcaa gttcataaac tgtatttcaa gacccgctgt tgaatatgtt ccacatggaa 3781 aacttccctc agaaagtgac tatctaatca cccactccat gaactggaaa accagcaaca 3841 agagaaagcg caaaatcact gatagtcata tctgtcttaa aaaatgtcta cttcacaaca 3901 tgtatcaaca atcaacagtt tatatcttgt cagacccctc tgagacttct ggaaaaatca 3961 ccattaaggc atcttttccc atagatgtat ttgtgaatcc tgcacacaac tacttttacc 4021 aggtggcgct ccaaaatatg gttgttcaag attacattga atttggtcag ggtgtatata 4081 aaagactagg ccatcagaag aatttcatag catctggatt tttcagaaag agacactttt 4141 ctgatcctgc tgtttgctcc attggtaatt gtggaaagtt ggatactaca gacgagatac 4201 cagtggcgct catcatcact cctgttagga ttccatctac tgtgctgcac gagtacctca 4261 agaaagcaat tgatttttgg aatcagtgtg ctgaggcaaa ctttgaccat atcccatggg 4321 ggcagctttc ggatgttgca agaaaaccca taacccccta atctagaggg cccgtttaaa 4381 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 4441 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 4501 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 4561 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 4621 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta 4681 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 4741 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 4801 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 4861 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 4921 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 4981 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 5041 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 5101 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 5161 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 5221 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 5281 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 5341 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 5401 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 5461 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc 5521 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg 5581 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc 5641 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc 5701 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc 5761 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc 5821 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc 5881 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga 5941 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 6001 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 6061 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 6121 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 6181 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 6241 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 6301 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 6361 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 6421 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 6481 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 6541 acagaatcag gggataacgc aggaaagaac a //
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