| 出品公司: | -- |
|---|---|
| 载体名称: | pSH63 |
| 质粒类型: | 酵母基因敲除载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | GAL |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6869 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
pSH63载体质粒基本信息
pSH63质粒图谱载体图谱和pSH63载体序列质粒序列多克隆位点信息
pSH63质粒载体简介
Plasmid is part of the " DEL-MARKER-SET ". For an individual plasmid order use accession number P30121.
Transformed E.coli cells are resistant to ampicillin. The plasmid contains the cre recombinase gene under control of a GAL promoter and complements the trp1 mutation in transformed Saccharomyces cerevisiae strains.
The pSH63 plasmid is usefull to express the cre recombinase under Galactose control. loxP flanked deletion markers will be removed from yeast deletions.
pSH63质粒序列载体序列
LOCUS AF298789 6869 bp DNA circular SYN 11-MAR-2002
DEFINITION Recombinase expression vector pSH63, complete sequence.
ACCESSION AF298789
VERSION AF298789.1 GI:11344895
KEYWORDS .
SOURCE recombinase expression vector pSH63
ORGANISM recombinase expression vector pSH63
artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6869)
AUTHORS Gueldener,U., Heinisch,J., Koehler,G.J., Voss,D. and Hegemann,J.H.
TITLE A second set of loxP marker cassettes for Cre-mediated multiple
gene knockouts in budding yeast
JOURNAL Nucleic Acids Res. 30 (6), E23 (2002)
MEDLINE 21882009
PUBMED 11884642
REFERENCE 2 (bases 1 to 6869)
AUTHORS Gueldener,U., Heinisch,J.H., Voss,D., Koehler,G. and Hegemann,J.H.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) Institut fuer Mikrobiologie,
Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf
40225, Germany
FEATURES Location/Qualifiers
source 1..6869
/organism="recombinase expression vector pSH63"
/mol_type="other DNA"
/db_xref="taxon:142966"
/note="derived from Saccharomyces cerevisiae"
gene 468..1142
/gene="TRP1"
CDS 468..1142
/gene="TRP1"
/codon_start=1
/transl_table=11
/product="phosphoribosylanthranilate isomerase"
/protein_id="AAG34533.1"
/db_xref="GI:11344896"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK"
terminator 1899..2152
/note="from CYC1"
gene complement(2470..3501)
/gene="Cre"
CDS complement(2470..3501)
/gene="Cre"
/codon_start=1
/transl_table=11
/product="recombinase"
/protein_id="AAG34534.1"
/db_xref="GI:11344897"
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM
LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN
LAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAK
DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRTLDSETGA
MVRLLEDGD"
promoter complement(3610..4077)
/note="from GAL1"
rep_origin 4520..4592
gene complement(5295..6155)
/gene="AmpR"
CDS complement(5295..6155)
/gene="AmpR"
/codon_start=1
/transl_table=11
/product="ampicillin resistance protein"
/protein_id="AAG34535.1"
/db_xref="GI:11344898"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1884 a 1538 c 1594 g 1852 t 1 others
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg
1921 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc
1981 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta
2041 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag
2101 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaggtc
2161 gacaattcca accttaccca agagttcgcc aaactcagac atcactttan gcaaaaccgc
2221 gccgtgcttc ttcctcggtg gcattcatca cgaaatgttc agcactacgc atacttttga
2281 caggaaacgc aacggatatt gagtcaatat caggcattct atcgctcagc tttacagtga
2341 caatgacggc tggcgactga atattagtgc ttacagacag cactacatat tttccgtcga
2401 tgttgaaatc ctttctcata tgtcaccata aatatcaaat aattatagca atcatttacg
2461 cgttaatggc taatcgccat cttccagcag gcgcaccatt gcccctgttt cactatccag
2521 ggtacggata tagttcatga caatatttac attggtccag ccaccagctt gcatgatctc
2581 cggtattgaa actccagcgc gggccatatc tcgcgcggct ccgacacggg cactgtgtcc
2641 agaccaggcc aggtatctct gaccagagtc atccttagcg ccgtaaatca atcgatgagt
2701 tgcttcaaaa atcccttcca gggcgcgagt tgatagctgg ctggtggcag atggcgcggc
2761 aacaccattt tttctgaccc ggcaaaacag gtagttattc ggatcatcag ctacaccaga
2821 gacggaaatc catcgctcga ccagtttagt tacccccagg ctaagtgcct tctctacacc
2881 tgcggtgcta accagcgttt tcgttctgcc aatatggatt aacattctcc caccgtcagt
2941 acgtgagata tctttaaccc tgatcctggc aatttcggct atacgtaaca gggtgttata
3001 agcaatcccc agaaatgcca gattacgtat atcctggcag cgatcgctat tttccatgag
3061 tgaacgaacc tggtcgaaat cagtgcgttc gaacgctaga gcctgttttg cacgttcacc
3121 ggcatcaacg ttttcttttc ggatccgccg cataaccagt gaaacagcat tgctgtcact
3181 tggtcgtggc agcccggacc gacgatgaag catgtttagc tggcccaaat gttgctggat
3241 agtttttact gccagaccgc gcgcctgaag atatagaaga taatcgcgaa catcttcagg
3301 ttctgcggga aaccatttcc ggttattcaa cttgcaccat gccgcccacg accggcaaac
3361 ggacagaagc attttccagg tatgctcaga aaacgcctgg cgatccctga acatgtccat
3421 caggttcttg cgaacctcat cactcgttgc atcgaccggt aatgcaggca aattttggtg
3481 tacggtcagt aaattggaca tttaacactc agataatggt tttaagtaaa gtgtacagga
3541 tcggctctgc ccctcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga
3601 tccactagtt ctagaatccg gggttttttc tccttgacgt taaagtatag aggtatatta
3661 acaatttttt gttgatactt ttattacatt tgaataagaa gtaatacaaa ccgaaaatgt
3721 tgaaagtatt agttaaagtg gttatgcagt ttttgcattt atatatctgt taatagatca
3781 aaaatcatcg cttcgctgat taattacccc agaaataagg ctaaaaaact aatcgcatta
3841 tcatcctatg gttgttaatt tgattcgttc atttgaaggt ttgtggggcc aggttactgc
3901 caatttttcc tcttcataac cataaaagct agtattgtag aatctttatt gttcggagca
3961 gtgcggcgcg aggcacatct gcgtttcagg aacgcgaccg gtgaagacga ggacgcacgg
4021 aggagagtct tccttcggag ggctgtcacc cgctcggcgg cttctaatcc gtactagagc
4081 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
4141 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatagg agccggaagc
4201 ataaagtgta aagcctgggg tgcctaatga gtgaggtaac tcacattaat tgcgttgcgc
4261 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
4321 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
4381 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
4441 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
4501 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
4561 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
4621 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
4681 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
4741 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
4801 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
4861 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
4921 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
4981 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
5041 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
5101 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
5161 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
5221 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
5281 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
5341 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
5401 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
5461 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
5521 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
5581 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
5641 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
5701 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
5761 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
5821 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
5881 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
5941 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
6001 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
6061 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
6121 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
6181 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
6241 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc cttttcatca
6301 cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa ttaaaaatag
6361 aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg taaaagactc
6421 tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc aggtattaat
6481 gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg tgattttaca
6541 ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt ctaataaata
6601 tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt ttttttcttc
6661 attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca aaacataaaa
6721 ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac gaggcgcgtg
6781 taagttacag gcaagcgatc cgtcctaaga aaccattatt atcatgacat taacctataa
6841 aaataggcgt atcacgaggc cctttcgtc
//
