| 质粒名称: | pInducer10-mir-RUP-PheS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 44011 |
| 存储实验室: | Stephen Elledge |
|
载体骨架基本信息 |
|
| 载体名称: | pGIPZ |
| 载体出品公司: | Open Biosystems |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 13167 bp |
| 完整质粒大小: | 14567 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒载体 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PheS Gly294,PheS |
| 物种来源: | E. coli |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | TRE2 |
| 标签/融合蛋白: | RFP（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | MluI（销毁后未知） |
| 3' 克隆位点: | BamHI（销毁后未知） |
| 5' 测序引物 | LNCX_primer |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pInducer10-mir-RUP-PheS was a gift from Stephen Elledge (Addgene plasmid # 44011 ; http://n2t.net/addgene:44011 ; RRID:Addgene_44011) |
| 参考文献部分: | The pINDUCER lentiviral toolkit for inducible RNA interference in vitro and in vivo. Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, Elledge SJ. Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9. 10.1073/pnas.1019736108 PubMed 21307310 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pInducer10-mir-RUP-PheS 14567 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral shRNA vector (pGIPz based) for inducible knockdown.
ACCESSION .
VERSION .
KEYWORDS pInducer10-mir-RUP-PheS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14567)
AUTHORS Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE,
Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi
RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF,
Elledge SJ
TITLE The pINDUCER lentiviral toolkit for inducible RNA interference in
vitro and in vivo.
JOURNAL Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi:
10.1073/pnas.1019736108. Epub 2011 Feb 9.
PUBMED 21307310
REFERENCE 2 (bases 1 to 14567)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..14567
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 497..730
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 915..959
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 1257..1374
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(1439..1813)
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
regulatory 1810..1819
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
promoter complement(1823..1841)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(1833..1880)
/label=EM7 promoter
/note="synthetic bacterial promoter "
protein_bind 1950..2220
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2253..2291
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2288..2312
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 2388..3083
/codon_start=1
/product="red fluorescent protein from Entacmaea
quadricolor"
/label=TurboRFP
/note="mammalian codon-optimized"
/translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
DLPSKLGHR"
ncRNA 3134..3227
/label=5' miR-30a
/note="sequence upstream of the 71-nt precursor of the
human miR-30a microRNA (Zeng et al., 2002)"
/ncRNA_class="miRNA"
primer_bind 3227..3243
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 3228..3244
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
CDS 3265..3282
/codon_start=1
/product="thrombin recognition and cleavage site"
/label=thrombin site
/translation="LVPRGS"
CDS 3283..3306
/codon_start=1
/product="recognition and cleavage site for human
rhinovirus 3C and PreScission proteases"
/label=HRV 3C site
/translation="LEVLFQGP"
regulatory 3316..3325
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4601..4621
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
ncRNA 4645..4757
/label=3' miR-30a
/note="sequence downstream of the 71-nt precursor of the
human miR-30a microRNA (Zeng et al., 2002)"
/ncRNA_class="miRNA"
promoter 4808..6019
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 5856..5875
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
protein_bind complement(6026..6059)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS 6066..6812
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/label=rtTA-Advanced
/note="In the Tet-On(R) system, rtTA-Advanced binds to the
Tet-responsive element and stimulates transcription only in
the presence of tetracycline or doxycycline."
/translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
protein_bind 6813..6846
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 6862..7434
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(7029..7046)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 7256..7275
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 7437..8039
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind 7936..7956
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 8061..8649
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8114..8134)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8532..8543)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 8856..9089
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(9190..9207)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9196..9420
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9466..9894
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9553..9572)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9763..9784
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(9903..9923)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9908..10237
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 10088..10223
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 10150..10169
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 11060..11181
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11230..11246)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11230..11246)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(11243..11265)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 11254..11270
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11278..11308)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11323..11344
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(11461..11478)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11632..12220)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11712..11731)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(12391..13251)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 13014..13033
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(13252..13356)
/gene="bla"
/label=AmpR promoter
polyA_signal 13404..13538
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
LTR 13706..14339
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
1 ccgagtaata ccgggggaga ttagatcgcg atgggaaaaa ttcggttaag gccaggggga
61 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
121 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
181 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
241 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
301 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
361 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
421 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
481 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
541 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
601 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
661 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
721 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
781 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
841 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
901 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
961 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
1021 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
1081 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
1141 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
1201 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt
1261 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
1321 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
1381 tattacaggg acagcagaga tccagtttgg ttagtaccgg gcccgctcta gtccggaatc
1441 agtcctgctc ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact
1501 cccgccccca cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt
1561 tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc
1621 aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt
1681 cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt
1741 cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg
1801 tcaacttggc catggtggcc ctcctatagt gagtcgtatt atactatgcc gatatactat
1861 gccgatgatt aattgtcaac acgtgctgca ggtccgaggt tctaggctag cttaaaggaa
1921 ccaattcagt cgactggatc ctttaccact ccctatcagt gatagagaaa agtgaaagtc
1981 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
2041 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
2101 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
2161 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
2221 aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
2281 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
2341 acctccatag aagacaccgg gaccgatcca gcctaccggt cgccaccatg agcgagctga
2401 tcaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca
2461 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg aagatcaagg
2521 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg
2581 gcagcaaagc cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc
2641 ctgagggctt cacatgggag agaatcacca catacgaaga cgggggcgtg ctgaccgcta
2701 cccaggacac cagcttccag aacggctgca tcatctacaa cgtcaagatc aacggggtga
2761 acttcccatc caacggccct gtgatgcaga agaaaacacg cggctgggag gccaacaccg
2821 agatgctgta ccccgctgac ggcggcctga gaggccacag ccagatggcc ctgaagctcg
2881 tgggcggggg ctacctgcac tgctccttca agaccacata cagatccaag aaacccgcta
2941 agaacctcaa gatgcccggc ttccacttcg tggaccacag actggaaaga atcaaggagg
3001 ccgacaaaga gacctacgtc gagcagcacg agatggctgt ggccaagtac tgcgacctcc
3061 ctagcaaact ggggcacaga tgatgcggcc gcaagccttg ttaagtgctc gcttcggcag
3121 cacatatact atgttgaatg aggcttcagt actttacaga atcgttgcct gcacatcttg
3181 gaaacacttg ctgggattac ttcttcaggt taacccaaca gaaggctcga ggtcgacggt
3241 atcgataagc ttgacgtcca tatgctggta ccccgtggaa gtctggaagt tctcttccag
3301 gggcccgaag gagacgccac catgggtcta gatagggata acagggtaat ggtaccggat
3361 cgatccccca acttccaggc ccgccatagt gatttgattt gccagcgcat cgctatcaat
3421 cgccgggttc acccattcgc gtaaccacag ttcactgaat ttcataatct attcctgcct
3481 tatttaaact gtttgaggaa acgcagatcg ttttcgaaga atgaacgcag gtcggtgacg
3541 ccgtaacgca acatagtcag acgctccatc cccatcccga agccgaaacc agagtaaact
3601 tccgggtcga tgccaacgtt acgcaacacg ttcggatgca ccatcccgca gcccagcact
3661 tccagccatt taccgttttt acccatgacg tccacttctg cagaaggttc ggtaaacggg
3721 aagtaggaag gacggaagcg aatctgcaaa tcttcctcaa agaagttacg caggaagtcg
3781 tgcagcgtgc ctttcaggtt ggtaaagctg atgttggtat caacaatcag accttccatc
3841 tgatggaaca tcggcgtgtg agtctggtcg tagtcgttac gataaacacg gccaggcgcg
3901 atgatacgaa tcggtggctg ctgggctttc atggtgcgga tctgtacgcc agaggtctgg
3961 gtacgcagca ggcgggtagt gtcaaaccag aaagtgtcgt ggtcagcgcg cgccgggtgg
4021 tgaccaggaa tgttcagagc atcgaagtta tgataatcgt cttcgatttc cggcccggtt
4081 gccacggtaa agccaagctc accgaagaaa ctttcgatac ggtcgatggt acgggtaacc
4141 ggatgcagac cgccgttttc aatgcgacga cctggcagag agacatcaat cgtttccgcc
4201 gccagacgcg cattcagtgc agcgctttcc agttccgctt tacgcgcatt cagcgcctgc
4261 tgaacctgct ctttcgcttc gttgataacc gcaccagctg ccggacgctc ttctggcggc
4321 agctcacgca gggtcgtcat ctgaagggtt aagtgccctt ttttacccaa atattcgacg
4381 cgcacattat ctaacgcggc aacatctgac gcctggctaa tggccgcctt cgcactggca
4441 accagttctg cgagatgtga catggttttc ctcattgtgt cagtggtgac actggttcgt
4501 tggacttaga gcctatccca tcaggctatt ttacttgcca ttttggtccc cgataccgtc
4561 gaccgcggat taccctgtta tccctagatc tcacaattct gagaatttgt attttcaggg
4621 tgatctccgt ggatctatta cgaattcaag gggctacttt aggagcaatt atcttgttta
4681 ctaaaactga ataccttgct atctctttga tacattttta caaagctgaa ttaaaatggt
4741 ataaattaaa tcactttttt caattggaag actaatgcgt ttaaacacgc ggcgacgcgt
4801 aagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg
4861 agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc
4921 aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac
4981 attttaggac gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca
5041 ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa
5101 cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga
5161 tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg
5221 ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag
5281 agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg
5341 gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg
5401 gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg
5461 aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg
5521 ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg
5581 cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg
5641 tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt
5701 aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct
5761 cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct
5821 ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg
5881 ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt
5941 tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt
6001 tttggctttt ttgttagacg gatccataac ttcgtataat gtatgctata cgaagttatg
6061 ccaccatgtc tagactggac aagagcaaag tcataaacgg agctctggaa ttactcaatg
6121 gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc
6181 ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga
6241 tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc
6301 tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag
6361 tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt
6421 tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc
6481 actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag
6541 agacacctac caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc
6601 ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga
6661 aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca
6721 tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg
6781 acgattttga ccttgacatg ctccccgggt aaataacttc gtatagcata cattatacga
6841 agttatggcg cgccaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
6901 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
6961 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
7021 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
7081 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
7141 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
7201 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
7261 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
7321 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
7381 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatgg
7441 ccaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
7501 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
7561 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
7621 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
7681 gcgtcgaagc gggggcggtg ttcgccgaga tcggctcgcg catggccgag ttgagcggtt
7741 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
7801 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
7861 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg
7921 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
7981 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat
8041 taattaagcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg
8101 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
8161 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
8221 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
8281 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
8341 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
8401 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
8461 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
8521 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
8581 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
8641 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag
8701 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
8761 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
8821 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg
8881 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
8941 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
9001 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
9061 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
9121 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gtttaaaccc
9181 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
9241 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
9301 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
9361 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
9421 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
9481 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
9541 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
9601 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
9661 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
9721 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
9781 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
9841 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
9901 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
9961 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
10021 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
10081 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
10141 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
10201 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
10261 cattttcgga tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtgaccgca
10321 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
10381 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
10441 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
10501 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
10561 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
10621 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
10681 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
10741 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
10801 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
10861 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
10921 aaaggaatag cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
10981 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
11041 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
11101 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
11161 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
11221 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
11281 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
11341 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
11401 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
11461 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
11521 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
11581 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
11641 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
11701 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
11761 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
11821 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
11881 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
11941 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
12001 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
12061 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
12121 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
12181 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
12241 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
12301 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
12361 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
12421 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
12481 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
12541 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
12601 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
12661 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
12721 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
12781 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
12841 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
12901 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
12961 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtccatac gggataatac
13021 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
13081 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
13141 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
13201 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
13261 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
13321 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
13381 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa
13441 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
13501 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
13561 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
13621 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
13681 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
13741 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
13801 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
13861 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
13921 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
13981 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
14041 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
14101 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
14161 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
14221 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
14281 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
14341 tgccgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
14401 actcggcttg ctgaagcgcg cacggcaaga ggcgaagggc ggcgactggt gagtacgcca
14461 aaaattttga ctagcggaag gctagaagga agaagatggt gccagagcgt cagtattaag
14521 cgggggaaaa ttagatcgcg aagggaaaaa attcggttaa ggccagg
//
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