质粒名称: | AAVS1-CAG-hrGFP |
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出品公司: | 爱迪基因 |
目录编号: | 52344 |
存储实验室: | Su-Chun Zhang |
载体骨架基本信息 |
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载体名称: | AAVS1-CAG |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | 8085 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | 嘌呤霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | hrGFP,Vitality hrGFP II |
物种来源: | Synthetic |
插入大小: | 724 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | CAG |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | SalI(未破坏) |
3' 克隆位点: | MluI (未破坏) |
5' 测序引物 | pCAX-F |
3' 测序引物 | Bglob-pA-R |
文献引用方法 |
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材料和方法部分: | AAVS1-CAG-hrGFP was a gift from Su-Chun Zhang (Addgene plasmid # 52344 ; http://n2t.net/addgene:52344 ; RRID:Addgene_52344) |
参考文献部分: | A simple and efficient system for regulating gene expression in human pluripotent stem cells and derivatives. Qian K, Huang CL, Chen H, Blackbourn LW 4th, Chen Y, Cao J, Yao L, Sauvey C, Du Z, Zhang SC. Stem Cells. 2014 Feb 4. doi: 10.1002/stem.1653. 10.1002/stem.1653 PubMed 24497442 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS AAVS1-CAG-hrGFP 9817 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION AAVS1 donor vector for constitutive gene expression. ACCESSION . VERSION . KEYWORDS AAVS1-CAG-hrGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9817) AUTHORS Qian K, Huang CL, Chen H, Blackbourn LW 4th, Chen Y, Cao J, Yao L, Sauvey C, Du Z, Zhang SC TITLE A simple and efficient system for regulating gene expression in human pluripotent stem cells and derivatives. JOURNAL Stem Cells. 2014 Feb 4. doi: 10.1002/stem.1653. PUBMED 24497442 REFERENCE 2 (bases 1 to 9817) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9817 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 37..840 /label=HA-L /note="left homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" misc_feature 847..872 /label=SA /note="splice acceptor site" CDS 896..949 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 959..1558 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(959..978) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 1455..1475 /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind complement(1590..1607) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1596..1820 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" enhancer 1826..2205 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2208..2483 /label=chicken beta-actin promoter intron 2484..3492 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 3416..3438 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" CDS 3557..4276 /codon_start=1 /product="humanized Renilla green fluorescent protein" /label=hrGFP /note="mammalian codon-optimized" /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE DGGLVEIRSDINLIEGMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI AQLTSLGKPLGSLHEWV" primer_bind complement(4378..4397) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 4443..4498 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(4497..4516) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(4859..4875) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 4883..4899 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4907..4937) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4952..4973 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." misc_feature 5047..5883 /label=HA-R /note="right homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" primer_bind complement(5923..5942) /label=T7 /note="T7 promoter, forward primer" promoter complement(5924..5942) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5949..5966) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(5949..5965) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5958..5980) /label=M13/pUC Forward /note="In lacZ gene" CDS 6103..6405 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 6284..6303 /label=ccdB-fwd /note="ccdB gene, forward primer" primer_bind complement(6808..6827) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7422..7441 /label=Neo-F /note="Neomycin resistance gene, forward primer" CDS complement(7802..8662) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8425..8444 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8786..9374 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9275..9294 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9528..9545 /label=L4440 /note="L4440 vector, forward primer" protein_bind 9662..9683 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9698..9728 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9736..9752 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9760..9776 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 9797..9815 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" ORIGIN 1 ctagctagtc ctgcaggttt aaacgaattc gccctttgct ttctctgacc agcattctct 61 cccctgggcc tgtgccgctt tctgtctgca gcttgtggcc tgggtcacct ctacggctgg 121 cccagatcct tccctgccgc ctccttcagg ttccgtcttc ctccactccc tcttcccctt 181 gctctctgct gtgttgctgc ccaaggatgc tctttccgga gcacttcctt ctcggcgctg 241 caccacgtga tgtcctctga gcggatcctc cccgtgtctg ggtcctctcc gggcatctct 301 cctccctcac ccaaccccat gccgtcttca ctcgctgggt tcccttttcc ttctccttct 361 ggggcctgtg ccatctctcg tttcttagga tggccttctc cgacggatgt ctcccttgcg 421 tcccgcctcc ccttcttgta ggcctgcatc atcaccgttt ttctggacaa ccccaaagta 481 ccccgtctcc ctggctttag ccacctctcc atcctcttgc tttctttgcc tggacacccc 541 gttctcctgt ggattcgggt cacctctcac tcctttcatt tgggcagctc ccctaccccc 601 cttacctctc tagtctgtgc tagctcttcc agccccctgt catggcatct tccaggggtc 661 cgagagctca gctagtcttc ttcctccaac ccgggcccct atgtccactt caggacagca 721 tgtttgctgc ctccagggat cctgtgtccc cgagctggga ccaccttata ttcccagggc 781 cggttaatgt ggctctggtt ctgggtactt ttatctgtcc cctccacccc acagtggggc 841 aagcttctga cctcttctct tcctcccaca gggcctcgag agatctggca gcggagaggg 901 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta ggctcgagat 961 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 1021 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 1081 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 1141 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 1201 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 1261 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 1321 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 1381 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 1441 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 1501 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatc 1561 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 1621 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 1681 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 1741 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 1801 ggatgcggtg ggctctatgg gtctcgacat tgattattga ctagttatta atagtaatca 1861 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta 1921 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat 1981 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga ctatttacgg 2041 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac 2101 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt 2161 cctacttggc agtacatcta cgtattagtc atcgctatta ccatgggtcg aggtgagccc 2221 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 2281 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc gcgccaggcg 2341 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc 2401 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata 2461 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt gccccgctcc 2521 gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg 2581 ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggctcgttt 2641 cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg tgcggggggg 2701 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggcccgcgc 2761 tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcgtgtgcg 2821 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga ggggaacaaa 2881 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc ggcggtcggg 2941 ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc 3001 ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt 3061 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg 3121 cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg 3181 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag ccgaaatctg 3241 ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg ccggcaggaa 3301 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccatctcca 3361 gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag ggcggggttc 3421 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc 3481 tttttcctac agctcctggg caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa 3541 gaattcgtcg accaccatgg tgagcaagca gatcctgaag aacaccggcc tgcaggagat 3601 catgagcttc aaggtgaacc tggagggcgt ggtgaacaac cacgtgttca ccatggaggg 3661 ctgcggcaag ggcaacatcc tgttcggcaa ccagctggtg cagatccgcg tgaccaaggg 3721 cgcccccctg cccttcgcct tcgacatcct gagccccgcc ttccagtacg gcaaccgcac 3781 cttcaccaag taccccgagg acatcagcga cttcttcatc cagagcttcc ccgccggctt 3841 cgtgtacgag cgcaccctgc gctacgagga cggcggcctg gtggagatcc gcagcgacat 3901 caacctgatc gaggggatgt tcgtgtaccg cgtggagtac aagggccgca acttccccaa 3961 cgacggcccc gtgatgaaga agaccatcac cggcctgcag cccagcttcg aggtggtgta 4021 catgaacgac ggcgtgctgg tgggccaggt gatcctggtg taccgcctga acagcggcaa 4081 gttctacagc tgccacatgc gcaccctgat gaagagcaag ggcgtggtga aggacttccc 4141 cgagtaccac ttcatccagc accgcctgga gaagacctac gtggaggacg gcggcttcgt 4201 agagcagcac gagaccgcca tcgcccagct gaccagcctg ggcaagcccc tgggcagcct 4261 gcacgagtgg gtgtaaatcg atgatatcac gcgtgaattc actcctcagg tgcaggctgc 4321 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct 4381 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg 4441 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact 4501 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag 4561 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca 4621 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg 4681 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt 4741 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc 4801 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt 4861 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 4921 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 4981 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc gacagtacta 5041 agctttacta gggacaggat tggtgacaga aaagccccat ccttaggcct cctccttcct 5101 agtctcctga tattgggtct aacccccacc tcctgttagg cagattcctt atctggtgac 5161 acacccccat ttcctggagc catctctctc cttgccagaa cctctaaggt ttgcttacga 5221 tggagccaga gaggatcctg ggagggagag cttggcaggg ggtgggaggg aaggggggga 5281 tgcgtgacct gcccggttct cagtggccac cctgcgctac cctctcccag aacctgagct 5341 gctctgacgc ggctgtctgg tgcgtttcac tgatcctggt gctgcagctt ccttacactt 5401 cccaagagga gaagcagttt ggaaaaacaa aatcagaata agttggtcct gagttctaac 5461 tttggctctt cacctttcta gtccccaatt tatattgttc ctccgtgcgt cagttttacc 5521 tgtgagataa ggccagtagc cagccccgtc ctggcagggc tgtggtgagg aggggggtgt 5581 ccgtgtggaa aactcccttt gtgagaatgg tgcgtcctag gtgttcacca ggtcgtggcc 5641 gcctctactc cctttctctt tctccatcct tctttcctta aagagtcccc agtgctatct 5701 gggacatatt cctccgccca gagcagggtc ccgcttccct aaggccctgc tctgggcttc 5761 tgggtttgag tccttggcaa gcccaggaga ggcgctcagg cttccctgtc ccccttcctc 5821 gtccaccatc tcatgcccct ggctctcctg ccccttccct acaggggttc ctggctctgc 5881 tctaagggca agggcgaatt cgcggccgct aaattcaatt cgccctatag tgagtcgtat 5941 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 6001 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 6061 accgatcgcc cttcccaaca gttgcgcagc ctatacgtac ggcagtttaa ggtttacacc 6121 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg 6181 ccggggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc 6241 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat 6301 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa 6361 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggcatgaga 6421 ttatcaaaaa ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg 6481 tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca 6541 aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta 6601 tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc 6661 tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaagc 6721 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 6781 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 6841 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 6901 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg 6961 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 7021 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 7081 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 7141 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 7201 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 7261 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatgca 7321 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 7381 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 7441 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 7501 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattattaa 7561 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg 7621 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 7681 tacattcaaa tatgtatccg ctcatgagat tatcaaaaag gatcttcacc tagatccttt 7741 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 7801 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 7861 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 7921 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 7981 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 8041 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 8101 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 8161 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 8221 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 8281 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 8341 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 8401 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 8461 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 8521 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 8581 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 8641 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 8701 gttattgtct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 8761 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 8821 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 8881 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 8941 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 9001 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 9061 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 9121 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 9181 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 9241 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 9301 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 9361 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 9421 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 9481 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 9541 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 9601 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 9661 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 9721 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 9781 attacgccaa gctcagaatt aaccctcact aaaggga //
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