| 质粒名称: | PABE-7 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 115628 |
| 存储实验室: | Caixia Gao |
|
载体骨架基本信息 |
|
| 载体名称: | pJIT163 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达，CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | wtTadA-TadA7.10-nCas9-3*NLS |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | PABE-7 was a gift from Caixia Gao (Addgene plasmid # 115628 ; http://n2t.net/addgene:115628 ; RRID:Addgene_115628) |
| 参考文献部分: | Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. 10.1186/s13059-018-1443-z PubMed 29807545 |
买家导航
PABE7载体基本信息
PABE7载体图谱质粒图谱和多克隆位点信息
PABE7载体简介
PABE7载体序列
LOCUS PABE-7 10360 bp ds-DNA circular SYN 10-JAN-2019
DEFINITION Targeted A to G in wheat plants or monocotyledons protoplasts .
ACCESSION .
VERSION .
KEYWORDS PABE-7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10360)
AUTHORS Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
TITLE Expanded base editing in rice and wheat using a Cas9-adenosine
deaminase fusion.
JOURNAL Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
PUBMED 29807545
REFERENCE 2 (bases 1 to 10360)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 10, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10360
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 3203..7303
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
RBS 7216..7224
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 7316..7336
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7349..7369
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7382..7402
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 7460..7636
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
primer_bind complement(8190..8206)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8190..8206)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8203..8225)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8214..8230
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8238..8268)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8283..8304
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8421..8438)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8592..9180)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8672..8691)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9351..10211)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9974..9993
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10212..10316)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
1981 gtttggtgtt acttctgcaa agcttatgtc cgaggtggag ttctctcacg agtattggat
2041 gaggcacgct cttacacttg ctaagagagc ttgggacgaa agagaagtgc cagttggcgc
2101 cgttcttgtg cataataata gggtgatcgg cgagggttgg aatagaccaa ttggaaggca
2161 tgatccaaca gctcacgcag agattatggc tctcagacaa ggcggcctcg ttatgcagaa
2221 ctacaggctc attgacgcta cactctacgt gacactcgaa ccttgcgtta tgtgcgccgg
2281 agctatgatt cattctagga ttggcagggt cgtgtttgga gctagggacg ctaaaacagg
2341 agccgccgga tctcttatgg acgtgttgca tcatccaggc atgaaccata gggtggagat
2401 tacagagggc attcttgcag acgagtgcgc tgctcttctt tccgatttct tcaggatgag
2461 aaggcaggag attaaggccc agaagaaggc tcagtcttct acagatagcg gaggatcttc
2521 cggaggatct agcggctccg agacaccagg aacatccgaa agcgctacac cagaatctag
2581 cggaggctct tccggaggat cttctgaagt ggagttctcc cacgagtatt ggatgaggca
2641 cgctcttaca cttgctaaaa gggctaggga cgaaagggaa gttccagttg gagctgttct
2701 cgtgctcaat aacagggtga ttggcgaggg ttggaataga gccattggac tccatgatcc
2761 aacagctcac gcagagatta tggctcttag acaaggcggc ctcgttatgc agaattacag
2821 actcatcgac gccacactct acgttacctt cgaaccttgc gttatgtgcg ccggagctat
2881 gatccattct aggattggca gggtcgtgtt cggcgttaga aacgctaaga caggagctgc
2941 aggctctctt atggacgttc ttcattaccc aggcatgaat catagagtgg agatcacaga
3001 aggcattctt gcagacgagt gcgcagctct cctttgctat ttcttcagga tgccgaggca
3061 agttttcaac gctcagaaga aggcccagtc ttctacagat tccggcggat cttctggagg
3121 atctagcggc tccgagacac caggaacatc cgaatccgct acaccagagt cttctggagg
3181 atctagcgga ggatctctta aggacaagaa gtactcgatc ggcctcgcca ttgggactaa
3241 ctctgttggc tgggccgtga tcaccgacga gtacaaggtg ccctcaaaga agttcaaggt
3301 cctgggcaac accgatcggc attccatcaa gaagaatctc attggcgctc tcctgttcga
3361 cagcggcgag acggctgagg ctacgcggct caagcgcacc gcccgcaggc ggtacacgcg
3421 caggaagaat cgcatctgct acctgcagga gattttctcc aacgagatgg cgaaggttga
3481 cgattctttc ttccacaggc tggaggagtc attcctcgtg gaggaggata agaagcacga
3541 gcggcatcca atcttcggca acattgtcga cgaggttgcc taccacgaga agtaccctac
3601 gatctaccat ctgcggaaga agctcgtgga ctccacagat aaggcggacc tccgcctgat
3661 ctacctcgct ctggcccaca tgattaagtt caggggccat ttcctgatcg agggggatct
3721 caacccggac aatagcgatg ttgacaagct gttcatccag ctcgtgcaga cgtacaacca
3781 gctcttcgag gagaacccca ttaatgcgtc aggcgtcgac gcgaaggcta tcctgtccgc
3841 taggctctcg aagtctcggc gcctcgagaa cctgatcgcc cagctgccgg gcgagaagaa
3901 gaacggcctg ttcgggaatc tcattgcgct cagcctgggg ctcacgccca acttcaagtc
3961 gaatttcgat ctcgctgagg acgccaagct gcagctctcc aaggacacat acgacgatga
4021 cctggataac ctcctggccc agatcggcga tcagtacgcg gacctgttcc tcgctgccaa
4081 gaatctgtcg gacgccatcc tcctgtctga tattctcagg gtgaacaccg agattacgaa
4141 ggctccgctc tcagcctcca tgatcaagcg ctacgacgag caccatcagg atctgaccct
4201 cctgaaggcg ctggtcaggc agcagctccc cgagaagtac aaggagatct tcttcgatca
4261 gtcgaagaac ggctacgctg ggtacattga cggcggggcc tctcaggagg agttctacaa
4321 gttcatcaag ccgattctgg agaagatgga cggcacggag gagctgctgg tgaagctcaa
4381 tcgcgaggac ctcctgagga agcagcggac attcgataac ggcagcatcc cacaccagat
4441 tcatctcggg gagctgcacg ctatcctgag gaggcaggag gacttctacc ctttcctcaa
4501 ggataaccgc gagaagatcg agaagattct gactttcagg atcccgtact acgtcggccc
4561 actcgctagg ggcaactccc gcttcgcttg gatgacccgc aagtcagagg agacgatcac
4621 gccgtggaac ttcgaggagg tggtcgacaa gggcgctagc gctcagtcgt tcatcgagag
4681 gatgacgaat ttcgacaaga acctgccaaa tgagaaggtg ctccctaagc actcgctcct
4741 gtacgagtac ttcacagtct acaacgagct gactaaggtg aagtatgtga ccgagggcat
4801 gaggaagccg gctttcctgt ctggggagca gaagaaggcc atcgtggacc tcctgttcaa
4861 gaccaaccgg aaggtcacgg ttaagcagct caaggaggac tacttcaaga agattgagtg
4921 cttcgattcg gtcgagatct ctggcgttga ggaccgcttc aacgcctccc tggggaccta
4981 ccacgatctc ctgaagatca ttaaggataa ggacttcctg gacaacgagg agaatgagga
5041 tatcctcgag gacattgtgc tgacactcac tctgttcgag gaccgggaga tgatcgagga
5101 gcgcctgaag acttacgccc atctcttcga tgacaaggtc atgaagcagc tcaagaggag
5161 gaggtacacc ggctggggga ggctgagcag gaagctcatc aacggcattc gggacaagca
5221 gtccgggaag acgatcctcg acttcctgaa gagcgatggc ttcgcgaacc gcaatttcat
5281 gcagctgatt cacgatgaca gcctcacatt caaggaggat atccagaagg ctcaggtgag
5341 cggccagggg gactcgctgc acgagcatat cgcgaacctc gctggctcgc cagctatcaa
5401 gaaggggatt ctgcagaccg tgaaggttgt ggacgagctg gtgaaggtca tgggcaggca
5461 caagcctgag aacatcgtca ttgagatggc ccgggagaat cagaccacgc agaagggcca
5521 gaagaactca cgcgagagga tgaagaggat cgaggagggc attaaggagc tggggtccca
5581 gatcctcaag gagcacccgg tggagaacac gcagctgcag aatgagaagc tctacctgta
5641 ctacctccag aatggccgcg atatgtatgt ggaccaggag ctggatatta acaggctcag
5701 cgattacgac gtcgatcata tcgttccaca gtcattcctg aaggatgact ccattgacaa
5761 caaggtcctc accaggtcgg acaagaaccg gggcaagtct gataatgttc cttcagagga
5821 ggtcgttaag aagatgaaga actactggcg ccagctcctg aatgccaagc tgatcacgca
5881 gcggaagttc gataacctca caaaggctga gaggggcggg ctctctgagc tggacaaggc
5941 gggcttcatc aagaggcagc tggtcgagac acggcagatc actaagcacg ttgcgcagat
6001 tctcgactca cggatgaaca ctaagtacga tgagaatgac aagctgatcc gcgaggtgaa
6061 ggtcatcacc ctgaagtcaa agctcgtctc cgacttcagg aaggatttcc agttctacaa
6121 ggttcgggag atcaacaatt accaccatgc ccatgacgcg tacctgaacg cggtggtcgg
6181 cacagctctg atcaagaagt acccaaagct cgagagcgag ttcgtgtacg gggactacaa
6241 ggtttacgat gtgaggaaga tgatcgccaa gtcggagcag gagattggca aggctaccgc
6301 caagtacttc ttctactcta acattatgaa tttcttcaag acagagatca ctctggccaa
6361 tggcgagatc cggaagcgcc ccctcatcga gacgaacggc gagacggggg agatcgtgtg
6421 ggacaagggc agggatttcg cgaccgtcag gaaggttctc tccatgccac aagtgaatat
6481 cgtcaagaag acagaggtcc agactggcgg gttctctaag gagtcaattc tgcctaagcg
6541 gaacagcgac aagctcatcg cccgcaagaa ggactgggat ccgaagaagt acggcgggtt
6601 cgacagcccc actgtggcct actcggtcct ggttgtggcg aaggttgaga agggcaagtc
6661 caagaagctc aagagcgtga aggagctgct ggggatcacg attatggagc gctccagctt
6721 cgagaagaac ccgatcgatt tcctggaggc gaagggctac aaggaggtga agaaggacct
6781 gatcattaag ctccccaagt actcactctt cgagctggag aacggcagga agcggatgct
6841 ggcttccgct ggcgagctgc agaaggggaa cgagctggct ctgccgtcca agtatgtgaa
6901 cttcctctac ctggcctccc actacgagaa gctcaagggc agccccgagg acaacgagca
6961 gaagcagctg ttcgtcgagc agcacaagca ttacctcgac gagatcattg agcagatttc
7021 cgagttctcc aagcgcgtga tcctggccga cgcgaatctg gataaggtcc tctccgcgta
7081 caacaagcac cgcgacaagc caatcaggga gcaggctgag aatatcattc atctcttcac
7141 cctgacgaac ctcggcgccc ctgctgcttt caagtacttc gacacaacta tcgatcgcaa
7201 gaggtacaca agcactaagg aggtcctgga cgcgaccctc atccaccagt cgattaccgg
7261 cctctacgag acgcgcatcg acctgtctca gctcgggggc gactccggcg gcagcccaaa
7321 gaagaagcgg aaggtgtctg gaggttctcc taagaaaaag agaaaagtgt ccggcggctc
7381 cccgaagaag aagcgcaagg tgtaggaatt cggtacgctg aaatcaccag tctctctcta
7441 caaatctatc tctctctatt ttctccataa ataatgtgtg agtagtttcc cgataaggga
7501 aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
7561 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
7621 tactaaaatc cagatctcct aaagtcccta tagatctttg tcgtgaatat aaaccagaca
7681 cgagacgact aaacctggag cccagacgcc gttcgaagct agaagtaccg cttaggcagg
7741 aggccgttag ggaaaagatg ctaaggcagg gttggttacg ttgactcccc cgtaggtttg
7801 gtttaaatat gatgaagtgg acggaaggaa ggaggaagac aaggaaggat aaggttgcag
7861 gccctgtgca aggtaagaag atggaaattt gatagaggta cgctactata cttatactat
7921 acgctaaggg aatgcttgta tttataccct atacccccta ataacccctt atcaatttaa
7981 gaaataatcc gcataagccc ccgcttaaaa attggtatca gagccatgaa taggtctatg
8041 accaaaactc aagaggataa aacctcacca aaatacgaaa gagttcttaa ctctaaagat
8101 aaaagatctt tcaagatcaa aactagttcc ctcacaccgg tgacggggat cgcatgcgat
8161 atctcgagat ctagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
8221 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
8281 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
8341 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
8401 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
8461 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
8521 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
8581 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
8641 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
8701 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
8761 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
8821 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
8881 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
8941 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
9001 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
9061 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
9121 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
9181 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
9241 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
9301 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
9361 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
9421 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
9481 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
9541 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
9601 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
9661 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
9721 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
9781 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
9841 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
9901 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
9961 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
10021 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
10081 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
10141 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
10201 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
10261 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
10321 tttccccgaa aagtgccacc tgccagtgcc aagctaattc
//
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