| 质粒名称: | PABE-5 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 115626 |
| 存储实验室: | Caixia Gao |
|
载体骨架基本信息 |
|
| 载体名称: | pJIT163 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达，CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | NLS-nCas9-NLS-wtTadA-TadA7.10-NLS |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | PABE-5 was a gift from Caixia Gao (Addgene plasmid # 115626 ; http://n2t.net/addgene:115626 ; RRID:Addgene_115626) |
| 参考文献部分: | Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. 10.1186/s13059-018-1443-z PubMed 29807545 |
买家导航
PABE5载体基本信息
PABE5载体图谱质粒图谱和多克隆位点信息
PABE5载体简介
PABE5载体序列
LOCUS PABE-5 10362 bp ds-DNA circular SYN 10-JAN-2019
DEFINITION Targeted A to G in wheat plants or monocotyledons protoplasts .
ACCESSION .
VERSION .
KEYWORDS PABE-5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10362)
AUTHORS Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
TITLE Expanded base editing in rice and wheat using a Cas9-adenosine
deaminase fusion.
JOURNAL Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
PUBMED 29807545
REFERENCE 2 (bases 1 to 10362)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 10, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10362
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 996..1016
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1029..5129
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
RBS 5042..5050
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 5136..5156
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6345..6392
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
polyA_signal 6450..6626
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
primer_bind complement(7180..7196)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7180..7196)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7193..7215)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7204..7220
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7228..7258)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7273..7294
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7411..7428)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7582..8170)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7662..7681)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8341..9201)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8964..8983
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9202..9306)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cctctctacc ttctctagat cggcgttccg gtccatggtt agggcccggt agttctactt
61 ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca
121 cggatgcgac ctgtacgtca gacacgttct gattgctaac ttgccagtgt ttctctttgg
181 ggaatcctgg gatggctcta gccgttccgc agacgggatc gatttcatga ttttttttgt
241 ttcgttgcat agggtttggt ttgccctttt cctttatttc aatatatgcc gtgcacttgt
301 ttgtcgggtc atcttttcat gctttttttt gtcttggttg tgatgatgtg gtctggttgg
361 gcggtcgttc tagatcggag tagaattaat tctgtttcaa actacctggt ggatttatta
421 attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa gatgatggat
481 ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat gcatatacag
541 agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt
601 tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat tttggaactg
661 tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa tatcgatcta
721 ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg catatgcagc
781 atctattcat atgctctaac cttgagtacc tatctattat aataaacaag tatgttttat
841 aattattttg atcttgatat acttggatga tggcatatgc agcagctata tgtggatttt
901 tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg tcgatgctca
961 ccctgttgtt tggtgttact tctgcaaagc ttatgccaaa aaagaagaga aaggtttcag
1021 gcggctccga caagaagtac tcgatcggcc tcgccattgg gactaactct gttggctggg
1081 ccgtgatcac cgacgagtac aaggtgccct caaagaagtt caaggtcctg ggcaacaccg
1141 atcggcattc catcaagaag aatctcattg gcgctctcct gttcgacagc ggcgagacgg
1201 ctgaggctac gcggctcaag cgcaccgccc gcaggcggta cacgcgcagg aagaatcgca
1261 tctgctacct gcaggagatt ttctccaacg agatggcgaa ggttgacgat tctttcttcc
1321 acaggctgga ggagtcattc ctcgtggagg aggataagaa gcacgagcgg catccaatct
1381 tcggcaacat tgtcgacgag gttgcctacc acgagaagta ccctacgatc taccatctgc
1441 ggaagaagct cgtggactcc acagataagg cggacctccg cctgatctac ctcgctctgg
1501 cccacatgat taagttcagg ggccatttcc tgatcgaggg ggatctcaac ccggacaata
1561 gcgatgttga caagctgttc atccagctcg tgcagacgta caaccagctc ttcgaggaga
1621 accccattaa tgcgtcaggc gtcgacgcga aggctatcct gtccgctagg ctctcgaagt
1681 ctcggcgcct cgagaacctg atcgcccagc tgccgggcga gaagaagaac ggcctgttcg
1741 ggaatctcat tgcgctcagc ctggggctca cgcccaactt caagtcgaat ttcgatctcg
1801 ctgaggacgc caagctgcag ctctccaagg acacatacga cgatgacctg gataacctcc
1861 tggcccagat cggcgatcag tacgcggacc tgttcctcgc tgccaagaat ctgtcggacg
1921 ccatcctcct gtctgatatt ctcagggtga acaccgagat tacgaaggct ccgctctcag
1981 cctccatgat caagcgctac gacgagcacc atcaggatct gaccctcctg aaggcgctgg
2041 tcaggcagca gctccccgag aagtacaagg agatcttctt cgatcagtcg aagaacggct
2101 acgctgggta cattgacggc ggggcctctc aggaggagtt ctacaagttc atcaagccga
2161 ttctggagaa gatggacggc acggaggagc tgctggtgaa gctcaatcgc gaggacctcc
2221 tgaggaagca gcggacattc gataacggca gcatcccaca ccagattcat ctcggggagc
2281 tgcacgctat cctgaggagg caggaggact tctacccttt cctcaaggat aaccgcgaga
2341 agatcgagaa gattctgact ttcaggatcc cgtactacgt cggcccactc gctaggggca
2401 actcccgctt cgcttggatg acccgcaagt cagaggagac gatcacgccg tggaacttcg
2461 aggaggtggt cgacaagggc gctagcgctc agtcgttcat cgagaggatg acgaatttcg
2521 acaagaacct gccaaatgag aaggtgctcc ctaagcactc gctcctgtac gagtacttca
2581 cagtctacaa cgagctgact aaggtgaagt atgtgaccga gggcatgagg aagccggctt
2641 tcctgtctgg ggagcagaag aaggccatcg tggacctcct gttcaagacc aaccggaagg
2701 tcacggttaa gcagctcaag gaggactact tcaagaagat tgagtgcttc gattcggtcg
2761 agatctctgg cgttgaggac cgcttcaacg cctccctggg gacctaccac gatctcctga
2821 agatcattaa ggataaggac ttcctggaca acgaggagaa tgaggatatc ctcgaggaca
2881 ttgtgctgac actcactctg ttcgaggacc gggagatgat cgaggagcgc ctgaagactt
2941 acgcccatct cttcgatgac aaggtcatga agcagctcaa gaggaggagg tacaccggct
3001 gggggaggct gagcaggaag ctcatcaacg gcattcggga caagcagtcc gggaagacga
3061 tcctcgactt cctgaagagc gatggcttcg cgaaccgcaa tttcatgcag ctgattcacg
3121 atgacagcct cacattcaag gaggatatcc agaaggctca ggtgagcggc cagggggact
3181 cgctgcacga gcatatcgcg aacctcgctg gctcgccagc tatcaagaag gggattctgc
3241 agaccgtgaa ggttgtggac gagctggtga aggtcatggg caggcacaag cctgagaaca
3301 tcgtcattga gatggcccgg gagaatcaga ccacgcagaa gggccagaag aactcacgcg
3361 agaggatgaa gaggatcgag gagggcatta aggagctggg gtcccagatc ctcaaggagc
3421 acccggtgga gaacacgcag ctgcagaatg agaagctcta cctgtactac ctccagaatg
3481 gccgcgatat gtatgtggac caggagctgg atattaacag gctcagcgat tacgacgtcg
3541 atcatatcgt tccacagtca ttcctgaagg atgactccat tgacaacaag gtcctcacca
3601 ggtcggacaa gaaccggggc aagtctgata atgttccttc agaggaggtc gttaagaaga
3661 tgaagaacta ctggcgccag ctcctgaatg ccaagctgat cacgcagcgg aagttcgata
3721 acctcacaaa ggctgagagg ggcgggctct ctgagctgga caaggcgggc ttcatcaaga
3781 ggcagctggt cgagacacgg cagatcacta agcacgttgc gcagattctc gactcacgga
3841 tgaacactaa gtacgatgag aatgacaagc tgatccgcga ggtgaaggtc atcaccctga
3901 agtcaaagct cgtctccgac ttcaggaagg atttccagtt ctacaaggtt cgggagatca
3961 acaattacca ccatgcccat gacgcgtacc tgaacgcggt ggtcggcaca gctctgatca
4021 agaagtaccc aaagctcgag agcgagttcg tgtacgggga ctacaaggtt tacgatgtga
4081 ggaagatgat cgccaagtcg gagcaggaga ttggcaaggc taccgccaag tacttcttct
4141 actctaacat tatgaatttc ttcaagacag agatcactct ggccaatggc gagatccgga
4201 agcgccccct catcgagacg aacggcgaga cgggggagat cgtgtgggac aagggcaggg
4261 atttcgcgac cgtcaggaag gttctctcca tgccacaagt gaatatcgtc aagaagacag
4321 aggtccagac tggcgggttc tctaaggagt caattctgcc taagcggaac agcgacaagc
4381 tcatcgcccg caagaaggac tgggatccga agaagtacgg cgggttcgac agccccactg
4441 tggcctactc ggtcctggtt gtggcgaagg ttgagaaggg caagtccaag aagctcaaga
4501 gcgtgaagga gctgctgggg atcacgatta tggagcgctc cagcttcgag aagaacccga
4561 tcgatttcct ggaggcgaag ggctacaagg aggtgaagaa ggacctgatc attaagctcc
4621 ccaagtactc actcttcgag ctggagaacg gcaggaagcg gatgctggct tccgctggcg
4681 agctgcagaa ggggaacgag ctggctctgc cgtccaagta tgtgaacttc ctctacctgg
4741 cctcccacta cgagaagctc aagggcagcc ccgaggacaa cgagcagaag cagctgttcg
4801 tcgagcagca caagcattac ctcgacgaga tcattgagca gatttccgag ttctccaagc
4861 gcgtgatcct ggccgacgcg aatctggata aggtcctctc cgcgtacaac aagcaccgcg
4921 acaagccaat cagggagcag gctgagaata tcattcatct cttcaccctg acgaacctcg
4981 gcgcccctgc tgctttcaag tacttcgaca caactatcga tcgcaagagg tacacaagca
5041 ctaaggaggt cctggacgcg accctcatcc accagtcgat taccggcctc tacgagacgc
5101 gcatcgacct gtctcagctc gggggcgacg aattcccaaa gaagaagcgc aaggtctccg
5161 gcggatcttc tggaggatct agcggctccg agacaccagg aacatccgaa tccgctacac
5221 cagagtcttc tggaggatct agcggaggat cttccgaggt ggagttctct cacgagtatt
5281 ggatgaggca cgctcttaca cttgctaaga gagcttggga cgaaagagaa gtgccagttg
5341 gcgccgttct tgtgcataat aatagggtga tcggcgaggg ttggaataga ccaattggaa
5401 ggcatgatcc aacagctcac gcagagatta tggctctcag acaaggcggc ctcgttatgc
5461 agaactacag gctcattgac gctacactct acgtgacact cgaaccttgc gttatgtgcg
5521 ccggagctat gattcattct aggattggca gggtcgtgtt tggagctagg gacgctaaaa
5581 caggagccgc cggatctctt atggacgtgt tgcatcatcc aggcatgaac catagggtgg
5641 agattacaga gggcattctt gcagacgagt gcgctgctct tctttccgat ttcttcagga
5701 tgagaaggca ggagattaag gcccagaaga aggctcagtc ttctacagat agcggaggat
5761 cttccggagg atctagcggc tccgagacac caggaacatc cgaaagcgct acaccagaat
5821 ctagcggagg ctcttccgga ggatcttctg aagtggagtt ctcccacgag tattggatga
5881 ggcacgctct tacacttgct aaaagggcta gggacgaaag ggaagttcca gttggagctg
5941 ttctcgtgct caataacagg gtgattggcg agggttggaa tagagccatt ggactccatg
6001 atccaacagc tcacgcagag attatggctc ttagacaagg cggcctcgtt atgcagaatt
6061 acagactcat cgacgccaca ctctacgtta ccttcgaacc ttgcgttatg tgcgccggag
6121 ctatgatcca ttctaggatt ggcagggtcg tgttcggcgt tagaaacgct aagacaggag
6181 ctgcaggctc tcttatggac gttcttcatt acccaggcat gaatcataga gtggagatca
6241 cagaaggcat tcttgcagac gagtgcgcag ctctcctttg ctatttcttc aggatgccga
6301 ggcaagtttt caacgctcag aagaaggccc agtcttctac agataagaga ccagcagcta
6361 caaagaaggc cggacaagct aagaagaaga agtaggaatt cggtacgctg aaatcaccag
6421 tctctctcta caaatctatc tctctctatt ttctccataa ataatgtgtg agtagtttcc
6481 cgataaggga aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
6541 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
6601 caaaatccag tactaaaatc cagatctcct aaagtcccta tagatctttg tcgtgaatat
6661 aaaccagaca cgagacgact aaacctggag cccagacgcc gttcgaagct agaagtaccg
6721 cttaggcagg aggccgttag ggaaaagatg ctaaggcagg gttggttacg ttgactcccc
6781 cgtaggtttg gtttaaatat gatgaagtgg acggaaggaa ggaggaagac aaggaaggat
6841 aaggttgcag gccctgtgca aggtaagaag atggaaattt gatagaggta cgctactata
6901 cttatactat acgctaaggg aatgcttgta tttataccct atacccccta ataacccctt
6961 atcaatttaa gaaataatcc gcataagccc ccgcttaaaa attggtatca gagccatgaa
7021 taggtctatg accaaaactc aagaggataa aacctcacca aaatacgaaa gagttcttaa
7081 ctctaaagat aaaagatctt tcaagatcaa aactagttcc ctcacaccgg tgacggggat
7141 cgcatgcgat atctcgagat ctagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
7201 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
7261 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
7321 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
7381 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
7441 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
7501 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
7561 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
7621 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
7681 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
7741 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
7801 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
7861 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
7921 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
7981 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
8041 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
8101 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
8161 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
8221 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
8281 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
8341 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
8401 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
8461 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
8521 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
8581 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
8641 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
8701 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
8761 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
8821 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
8881 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
8941 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
9001 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
9061 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
9121 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
9181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
9241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
9301 tccgcgcaca tttccccgaa aagtgccacc tgccagtgcc aagctaattc gagctcggta
9361 cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa gttataaaaa
9421 attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat ctttatacat
9481 atatttaaac tttactctac gaataatata atctatagta ctacaataat atcagtgttt
9541 tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag tattttgaca
9601 acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt tttgcaaata
9661 gcttcaccta tataatactt catccatttt attagtacat ccatttaggg tttagggtta
9721 atggttttta tagactaatt tttttagtac atctatttta ttctatttta gcctctaaat
9781 taagaaaact aaaactctat tttagttttt ttatttaata atttagatat aaaatagaat
9841 aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa actaaggaaa
9901 catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac gagtctaacg
9961 gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat
10021 ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt tggacttgct
10081 ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc
10141 ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc accgctcctt
10201 cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca
10261 acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg
10321 gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc cc
//
