| 质粒名称: | gRNA_Cloning Vector |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 41824 |
| 存储实验室: | George Church |
| 用途: | 一个空的gRNA表达载体，用于创建一个特定序列的基因组。 |
|
载体骨架基本信息 |
|
| 载体名称: | pCR-Blunt II-TOPO |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 卡那霉素 |
| 载体类型: | 哺乳细胞表达,CRISPR |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 克隆方法: | TOPO克隆 |
| 5' 测序引物 | T7 |
|
文献引用方法 |
|
| 材料和方法部分: | gRNA_Cloning Vector was a gift from George Church (Addgene plasmid # 41824) |
| 参考文献部分: | RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science. 2013 Jan 3. 10.1126/science.1232033 PubMed 23287722 |
买家导航
gRNA_Cloning Vector载体质粒基本信息
gRNA_Cloning Vector质粒图谱载体图谱和gRNA_Cloning Vector载体序列质粒序列多克隆位点信息
gRNA_Cloning Vector质粒载体简介
gRNA_Cloning Vector质粒序列载体序列
LOCUS gRNA_Cloning Vector Sequencing Result 3915 bp DNA SYN
DEFINITION gRNA_Cloning Vector Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3915
/organism="gRNA_Cloning Vector Sequencing Result"
/mol_type="other DNA"
promoter 143..172
/label="lac_promoter"
misc_feature 186..208
/label="M13_pUC_rev_primer"
promoter 207..225
/label="M13_reverse_primer"
misc_feature 239..256
/label="Sp6_primer"
promoter complement(434..678)
/label="hU6_promoter"
misc_feature complement(473..492)
/label="LKO1_5_primer"
promoter complement(803..821)
/label="T7_promoter"
misc_feature 825..968
/label="lacZ_a"
promoter complement(828..844)
/label="M13_forward20_primer"
misc_feature complement(837..859)
/label="M13_pUC_fwd_primer"
misc_feature 991..1284
/label="ccdB"
promoter 1495..1544
/label="NEOKAN_promoter"
CDS 1633..2427
/label="ORF frame 1"
gene 1636..2424
/label="NeoR/KanR"
/gene="NeoR/KanR"
CDS complement(1942..2475)
/label="ORF frame 1"
promoter 2561..2589
/label="AmpR_promoter"
gene 2634..3005
/label="bleo"
/gene="bleo"
misc_feature 2634..3008
/label="sh_ble"
rep_origin 3130..3749
/label="pBR322_origin"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
301 ctagtaacgg ccgccagtgt gctggaattc gccctttaat gccaactttg tacaagaaag
361 ctgggtctag aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc
421 cttattttaa cttaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag
481 catatgatag tccattttaa aacataattt taaaactgca aactacccaa gaaattatta
541 ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattctaat
601 tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc
661 ctcttcctgc ccgaccttgg taccggatcc agtcgactga attggttcct ttaaagcctg
721 cttttttgta caaagggcga attctgcaga tatccatcac actggcggcc gctcgagcat
781 gcatctagag ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
841 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
901 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
961 ttgcgcagcc tatacgtacg gcagtttaag gtttacacct ataaaagaga gagccgttat
1021 cgtctgtttg tggatgtaca gagtgatatt attgacacgc cggggcgacg gatggtgatc
1081 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
1141 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
1201 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
1261 aacctgatgt tctggggaat ataaatgtca ggcatgagat tatcaaaaag gatcttcacc
1321 tagatccttt tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
1381 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc
1441 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa
1501 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
1561 ttctcgccgc caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga
1621 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
1681 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
1741 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
1801 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
1861 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
1921 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
1981 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
2041 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
2101 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
2161 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
2221 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
2281 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
2341 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
2401 tatcgccttc ttgacgagtt cttctgaatt attaacgctt acaatttcct gatgcggtat
2461 tttctcctta cgcatctgtg cggtatttca caccgcatac aggtggcact tttcggggaa
2521 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
2581 tgagacaata accctgataa atgcttcaat aatagcacgt gaggagggcc accatggcca
2641 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
2701 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
2761 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
2821 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
2881 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
2941 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
3001 aggactgaca cgtgctaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
3061 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
3121 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
3181 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
3241 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
3301 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
3361 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
3421 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
3481 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
3541 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
3601 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
3661 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
3721 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctgggc ttttgctggc
3781 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
3841 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
3901 gcgaggaagc ggaag
//
