| 出品人: | Irene Castano Navarro（2015） |
|---|---|
| 载体名称: | pYC66 |
| 质粒类型: | 酿酒酵母载体；复制型载体；抗性筛选载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 5085 bp |
| 5' 测序引物及序列: | M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’ |
| 3' 测序引物及序列: | M13 rev primer 5’ GGAAACAGCTATGACCATG 3’ |
| 载体标签: | 3XMyc tag (C-ter) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Nourseothricin |
| 克隆菌株: | DH10B感受态细胞 ,30°C |
| 宿主细胞（系）: | 出芽酵母（酿酒酵母）、白色念珠菌 |
| 备注: | -- |
| 产品目录号: | #63929 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pYC66载体质粒基本信息
pYC66质粒图谱载体图谱和pYC66载体序列质粒序列多克隆位点信息
pYC66质粒载体简介
pYC66质粒序列载体序列
LOCUS pYC66 5085 bp DNA SYN
DEFINITION pYC66
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5085
/organism="pYC66"
/mol_type="other DNA"
CDS 761..1333
/label="ORF frame 2"
/translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
terminator 1347..1586
/label="CYC1_terminator"
/translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
misc_feature complement(1356..1374)
/label="CYC1_primer"
/translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
misc_feature complement(1356..1374)
/label="pYESTrp_rev_primer"
/translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
misc_feature 1608..1624
/label="pBluescriptKS_primer"
/translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
promoter complement(2287..2306)
/label="T3_promoter"
/translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
promoter complement(2323..2341)
/label="M13_reverse_primer"
/translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
misc_feature complement(2340..2362)
/label="M13_pUC_rev_primer"
/translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
promoter complement(2376..2405)
/label="lac_promoter"
/translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
gene complement(3488..4348)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
CDS complement(3488..4348)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(4390..4418)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcaCctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cgGCATCAGA GCAGATTGTA CTGAGAGTGC
181 ACCATAccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
301 agcacagact tagattggta tatatacgCA TACcataaag cacAGCTCca tagcttcaaa
361 atgtttctac tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca
421 cttcaaaaca cccaagcaca gcatactaaa tttcccctct ttcttcctct agggtgtcgt
481 taattacccg tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc
541 gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt cttgaaaatt ttttttttga
601 tttttttctc tttcgatgac ctcccattga tatttaagtt aataaacggt cttcaatttc
661 tcaagtttca gtttcatttt tcttgttcta ttacaacttt ttttacttct tgctcattag
721 aaagaaagca tagcaatcta atctaagttt tggatctaaa ATGGGCACCA CTCTTGACGA
781 CACGGCTTAC CGATACCGCA CCAGTGTCCC GGGGGACGCC GAGGCCATCG AGGCACTGGA
841 TGGGTCCTTC ACCACCGACA CCGTCTTCCG CGTCACCGCC ACCGGGGACG GCTTCACCCT
901 GCGGGAGGTG CCGGTGGACC CGCCCCTGAC CAAGGTGTTC CCCGACGACG AATCGGACGA
961 CGAATCGGAC GACGGGGAGG ACGGCGACCC TGACTCCCGG ACGTTCGTCG CGTACGGGGA
1021 CGACGGCGAC CTGGCGGGCT TCGTGGTCGT CTCGTACTCC GGCTGGAACC GCCGGCTGAC
1081 CGTCGAGGAC ATCGAGGTCG CCCCGGAGCA CCGGGGGCAC GGGGTCGGGC GCGCGTTGAT
1141 GGGGCTCGCG ACGGAGTTCG CCCGCGAGCT GGGCGCCGGG CACCTCTGGC TGGAGGTCAC
1201 CAACGTCAAC GCACCGGCGA TCCACGCGTA CCGGCGGATG GGGTTCACCC TCTGCGGCCT
1261 GGACACCGCC CTGTACGACG GCACCGCCTC GGACGGCGAG CAGGCGCTCT ACATGAGCAT
1321 GCCCTGCCCC TAAgtcgagt catgtaatta gttatgtcac gcttacattc acgccctccc
1381 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
1441 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
1501 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
1561 ttgggacgct cgaaggcttt aatttgcggc cGGTACcggg ccccccctcg aggtcgacgg
1621 tatcgataag cttgatatcg aattcctgca gcccggggga tcctagggTT AATTAACGGT
1681 GAACAAAAGC TAATCTCCGA GGAAGACTTG AACGGTGAAC AAAAATTAAT CTCAGAAGAA
1741 GACTTGAACG GACTCGACGG TGAACAAAAG TTGATTTCTG AAGAAGATTT GAACGGTGAA
1801 CAAAAGCTAA TCTCCGAGGA AGACTTGAAC GGTGAACAAA AATTAATCTC AGAAGAAGAC
1861 TTGAACGGAC TCGACGGTGA ACAAAAGTTG ATTTCTGAAG AAGATTTGAA CGGTGAACAA
1921 AAGCTAATCT CCGAGGAAGA CTTGAACGGT GAACAAAAAT TAATCTCAGA AGAAGACTTG
1981 AACGGACTCG ACGGTGAACA AAAGTTGATT TCTGAAGAAG ATTTGAACGG TGAACAAAAG
2041 CTAATCTCCG AGGAAGACTT GAACGGTGAA CAAAAATTAA TCTCAGAAGA AGACTTGAAC
2101 GGACTCGACG GTGAACAAAA GTTGATTTCT GAAGAAGATT TGAACGGTGA ACAAAAGCTA
2161 ATCTCCGAGG AAGACTTGAA CGGTGAACAA AAATTAATCA ATCACtagct agtgaattcg
2221 cgccagatcc GAAGTTCCTA TACTTTctag agcggccgcc accgCGGTGG AGCTCCAGCT
2281 TTTGTTCCCT TTAGTGAGGG TTAATTGCGC GCTTGGCGTA ATCATGGTCA TAGCTGTTTC
2341 Ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat aCgagccgga agcataaagt
2401 gtaaagcctg gggtgcctaa tgagtgagCt aactcacatt aattgcgttg cgctcactgc
2461 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
2521 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
2581 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
2641 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
2701 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
2761 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
2821 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
2881 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
2941 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
3001 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
3061 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
3121 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
3181 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
3241 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
3301 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
3361 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
3421 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
3481 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
3541 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
3601 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
3661 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
3721 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
3781 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
3841 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
3901 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
3961 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
4021 gcttttctgt gactggtgAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC
4081 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA
4141 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT
4201 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT
4261 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA
4321 GGGCGACACG GAAATGTTGA ATACTCATac tcttcctttt tcaatattat tgaagcattt
4381 atcagggtta ttgtctcatg agcggataca taTTTGAATG TGGGGATCTC TAATGATGCA
4441 ATTTTTGTTT TGTACACTAA GATTCTATTT CGGAACTCTT ACCCATGTCA TTATTTactt
4501 tattttcatt attgTAATTT TTTAAGCTTT TATTATGAAT TATTTAAATA CAATTCTTTT
4561 TGATCATGTG ACAGACATCA CTGAAAGATG TGCTCTGCGT TATTAGTCAG ATTGAAATAT
4621 ACATTTTCAT GTAGATATGA TGTTCTTACT TTAAATGTTG TTCCATTTGC TAGCTTAAAT
4681 AGACGATATT ATAAATTTAA GTATTCACTA AAAATCTGAC CCATTAATGT TAGAATTAGT
4741 ATTATTTTTT GTGATATTAC ATTTTTATAT TTTGGATTTC AACCAATGCT CTGCGATATG
4801 ATTCAACAGA ATAATATGTA CAAGCTACTT TAATTCATAT ATACTAATAG ATTCACAGAT
4861 CCAGTACGAC TGCAAGAATT TACGTTTTGT TTGAAATGGT AGTAGCTACA CTAAATTAAC
4921 AATGATCGAT TAATCCACAT CCACAGTGTG TAATCATCCT AATGAAAACA AGAATAACTA
4981 CAAAAATGAA TATAAACTTT TTGCATGTTA GTCTAACCTG CTAAGAAACC ATTATTATCA
5041 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTC
//
