| 出品公司: | -- |
|---|---|
| 载体名称: | pCS107 |
| 质粒类型: | 克隆载体；哺乳动物表达载体；广宿主载体；体外表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | simian CMV IE94、lac、T3、SP6 |
| 载体大小: | 4131 bp |
| 5' 测序引物及序列: | SP6 fwd（MCS1）、T3 fwd（MCS2） |
| 3' 测序引物及序列: | EBV rev（MCS1）、SV40 polyA rev（MCS2） |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH10B |
| 表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
| 备注: | pCS107载体能够在多种细胞中进行高水平的瞬表达。pCS107载体 同时也能进行体外转录和翻译。 |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCS107载体质粒基本信息
pCS107质粒图谱载体图谱和pCS107载体序列质粒序列多克隆位点信息
pCS107质粒载体简介
pCS107质粒序列载体序列
LOCUS Exported 4131 bp ds-DNA circular SYN 29-JUL-2016
DEFINITION Vector that allows high-level transient expression in vertebrate
cells as well as in vitro transcription/translation.
ACCESSION DQ649432
KEYWORDS pCS107
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4131)
AUTHORS Biofeng Lab.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pCS107|
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..4131
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 35..53
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
misc_feature 79..140
/note="MCS1"
polyA_signal 172..306
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
misc_feature 375..410
/note="MCS2"
promoter complement(423..441)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature complement(464..466)
/note="ATG"
protein_bind 486..502
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(510..540)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(864..1452)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1623..2483)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2484..2588)
/gene="bla"
/note="AmpR promoter"
rep_origin complement(2614..3069)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3147..4131
/note="CMV IE94 promoter"
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
ORIGIN
1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg tcgacaggcc aatctggccg
121 cggccgcaag gcctctcgag cctctcgccc tatagtgagt cgtattacgt agatccagac
181 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
241 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
301 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
361 gttttttaat tcgcggcgcg ccgcggcgcc aatgcattgg gcccggtacc cagcttttgt
421 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
481 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
541 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
601 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
661 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
721 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
781 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
841 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
901 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
961 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
1021 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
1081 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
1141 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
1201 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
1261 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
1321 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
1381 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
1441 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
1501 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
1561 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
1621 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
1681 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
1741 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
1801 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
1861 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
1921 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
1981 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
2041 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
2101 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
2161 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
2221 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
2281 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
2341 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
2401 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
2461 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
2521 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
2581 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
2641 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
2701 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
2761 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
2821 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
2881 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
2941 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
3001 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
3061 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
3121 cctcttcgct attacgccag tcgatcgacc atagccaatt caatatggcg tatatggact
3181 catgccaatt caatatggtg gatctggacc tgtgccaatt caatatggcg tatatggact
3241 cgtgccaatt caatatggtg gatctggacc ccagccaatt caatatggcg gacttggcac
3301 catgccaatt caatatggcg gacttggcac tgtgccaact ggggaggggt ctacttggca
3361 cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag tccgccatat tgaattggca
3421 tggtgccaat aatggcggcc atattggcta tatgccagga tcaatatata ggcaatatcc
3481 aatatggccc tatgccaata tggctattgg ccaggttcaa tactatgtat tggccctatg
3541 ccatatagta ttccatatat gggttttcct attgacgtag atagcccctc ccaatgggcg
3601 gtcccatata ccatatatgg ggcttcctaa taccgcccat agccactccc ccattgacgt
3661 caatggtctc tatatatggt ctttcctatt gacgtcatat gggcggtcct attgacgtat
3721 atggcgcctc ccccattgac gtcaattacg gtaaatggcc cgcctggctc aatgcccatt
3781 gacgtcaata ggaccaccca ccattgacgt caatgggatg gctcattgcc cattcatatc
3841 cgttctcacg ccccctattg acgtcaatga cggtaaatgg cccacttggc agtacatcaa
3901 tatctattaa tagtaacttg gcaagtacat tactattgga agtacgccag ggtacattgg
3961 cagtactccc attgacgtca atggcggtaa atggcccgcg atggctgcca agtacatccc
4021 cattgacgtc aatggggagg ggcaatgacg caaatgggcg ttccattgac gtaaatgggc
4081 ggtaggcgtg cctaatggga ggtctatata agcaatgctc gtttagggaa c
//
