| 出品公司: | -- |
|---|---|
| 载体名称: | pCS108 |
| 质粒类型: | 克隆载体；哺乳动物表达载体；广宿主载体；体外表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac、T3、SP6、CMV IE94 |
| 载体大小: | 4149 bp |
| 5' 测序引物及序列: | SP6 fwd（MCS1）、T3 fwd（MCS2） |
| 3' 测序引物及序列: | EBV rev（MCS1）、SV40 polyA rev（MCS2） |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH10B |
| 表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
| 备注: | pCS108载体能够在多种细胞中进行高水平的瞬表达。pCS108载体同时也能进行体外转录和翻译。 |
| 产品目录号: | -- |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
pCS108载体质粒基本信息
pCS108质粒图谱载体图谱和pCS108载体序列质粒序列多克隆位点信息
pCS108质粒载体简介
pCS108质粒序列载体序列
LOCUS Exported 4149 bp ds-DNA circular SYN 29-JUL-2016
DEFINITION Vector that allows high-level transient expression in vertebrate
cells as well as in vitro transcription/translation.
ACCESSION DQ649433
VERSION .
KEYWORDS pCS108
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4149)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..4149
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 35..53
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
misc_feature 79..158
/note="MCS1"
polyA_signal 190..324
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
misc_feature 393..428
/note="MCS2"
promoter complement(441..459)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(480..496)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature complement(482..484)
/note="ATG"
protein_bind 504..520
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(528..558)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(882..1470)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1641..2501)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2502..2606)
/gene="bla"
/note="AmpR promoter"
rep_origin complement(2632..3087)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3165..4149
/note="CMV IE94 promoter"
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
ORIGIN
1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg tcgacaggcc aatctggccg
121 cggccgcaag gcctgatatc tctagaccgc ggctcgagcc tctcgcccta tagtgagtcg
181 tattacgtag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
241 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
301 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
361 cagggggagg tgtgggaggt tttttaattc gcggcgcgcc gcggcgccaa tgcattgggc
421 ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
481 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
541 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
601 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
661 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
721 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
781 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
1021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
1081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
1141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
1201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
1261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
1321 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
1381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
1441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
1501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
1561 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
1621 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
1681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
1741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
1801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
1861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
1921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
1981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
2041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
2101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
2161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
2221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
2281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
2341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
2401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
2461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
2521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
2581 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
2641 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
2701 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
2761 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
2821 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
2881 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
2941 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
3001 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
3061 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
3121 aagggcgatc ggtgcgggcc tcttcgctat tacgccagtc gatcgaccat agccaattca
3181 atatggcgta tatggactca tgccaattca atatggtgga tctggacctg tgccaattca
3241 atatggcgta tatggactcg tgccaattca atatggtgga tctggacccc agccaattca
3301 atatggcgga cttggcacca tgccaattca atatggcgga cttggcactg tgccaactgg
3361 ggaggggtct acttggcacg gtgccaagtt tgaggagggg tcttggccct gtgccaagtc
3421 cgccatattg aattggcatg gtgccaataa tggcggccat attggctata tgccaggatc
3481 aatatatagg caatatccaa tatggcccta tgccaatatg gctattggcc aggttcaata
3541 ctatgtattg gccctatgcc atatagtatt ccatatatgg gttttcctat tgacgtagat
3601 agcccctccc aatgggcggt cccatatacc atatatgggg cttcctaata ccgcccatag
3661 ccactccccc attgacgtca atggtctcta tatatggtct ttcctattga cgtcatatgg
3721 gcggtcctat tgacgtatat ggcgcctccc ccattgacgt caattacggt aaatggcccg
3781 cctggctcaa tgcccattga cgtcaatagg accacccacc attgacgtca atgggatggc
3841 tcattgccca ttcatatccg ttctcacgcc ccctattgac gtcaatgacg gtaaatggcc
3901 cacttggcag tacatcaata tctattaata gtaacttggc aagtacatta ctattggaag
3961 tacgccaggg tacattggca gtactcccat tgacgtcaat ggcggtaaat ggcccgcgat
4021 ggctgccaag tacatcccca ttgacgtcaa tggggagggg caatgacgca aatgggcgtt
4081 ccattgacgt aaatgggcgg taggcgtgcc taatgggagg tctatataag caatgctcgt
4141 ttagggaac
//
