| 出品公司: | -- |
|---|---|
| 载体名称: | pPZP221 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 8732 bp |
| 5' 测序引物及序列: | M13 Reverse：CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward：CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | 无 |
| 载体抗性: | 壮观霉素(or 链霉素) |
| 筛选标记: | 庆大霉素 |
| 克隆菌株: | HB101 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | 重组质粒可用蓝白斑法筛选 |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pPZP221载体质粒基本信息
pPZP221质粒图谱载体图谱和pPZP221载体序列质粒序列多克隆位点信息
pPZP221质粒载体简介
pPZP221质粒序列载体序列
LOCUS Exported 8732 bp ds-DNA circular SYN 28-JUL-2016
DEFINITION Agrobacterium binary vector for plant transformation, with
spectinomycin- and gentamycin-resistance genes. The MCS is reversed
in pPZP222.
ACCESSION U10491
VERSION .
KEYWORDS pPZP221
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8732)
AUTHORS Hajdukiewicz P, Svab Z, Maliga P.
TITLE The small, versatile pPZP family of Agrobacterium binary vectors for
plant transformation.
JOURNAL Plant Mol. Biol. 1994;25:989-94.
PUBMED 7919218
REFERENCE 2 (bases 1 to 8732)
AUTHORS .
TITLE Direct Submission
COMMENT The GenBank sequence was corrected by inserting a G at position
2249.
FEATURES Location/Qualifiers
source 1..8732
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
CDS 1187..1816
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/note="pVS1 StaA"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 2245..3318
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/note="pVS1 RepA"
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 3384..3578
/note="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 3922..4062
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(4248..4836)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5080..5871)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase"
/note="SmR"
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
misc_feature 6399..6423
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 6521..6695
/note="CaMV poly(A) signal"
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(6730..7263)
/codon_start=1
/gene="aacC1"
/product="gentamycin acetyltransferase"
/note="GmR"
/note="confers resistance to gentamycin"
/translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
EEVMHFDIDPSTAT"
promoter complement(7323..8001)
/note="35S promoter (enhanced)"
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
promoter 8228..8258
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 8266..8282
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8290..8306
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 8302..8535
/codon_start=1
/gene="lacZ (fragment)"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
misc_feature 8302..8304
/note="ATG"
misc_feature 8316..8372
/note="MCS"
/note="pUC18 multiple cloning site"
primer_bind complement(8376..8392)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 8595..8619
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
ORIGIN
1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata
4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc
4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aatcgcttga gttaacgccg
5041 gcgaagcggc gtcggcttga acgaatttct agctagacat tatttgccga ctaccttggt
5101 gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
5161 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
5221 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
5281 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
5341 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
5401 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
5461 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
5521 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
5581 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
5641 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
5701 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
5761 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
5821 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccccca tgatgtttaa
5881 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
5941 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa
6001 aaaacatgtc ataacaagaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
6061 cggtcaaggt tctggaccag ttgcgtgacg gcagttacgc tacttgcatt acagcttacg
6121 aaccgaacga ggcttatgtc cactgggttc gtgcccgaat tgatcacagg cagcaacgct
6181 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc
6241 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca
6301 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt
6361 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta
6421 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt
6481 aacgccgaat taattcgggg gatctatagg gactttaggt gatctggatt ttagtactgg
6541 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
6601 actaagggtt tcttatatgc tcaacacgtg agcgaaaccc tataagaacc ctaattccct
6661 tatctgggaa ctactcacac attattatgg agaaactcga tgtcgatcga ctctagctag
6721 aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat
6781 gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat
6841 cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat
6901 gccctgcctc cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg
6961 ctgctcaaac ctgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa
7021 ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg
7081 ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc
7141 ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga
7201 gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtac
7261 catggtcgat cgacagatct gcgaatcgag agagatagat ttgtagagag agactggtga
7321 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
7381 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
7441 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
7501 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
7561 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
7621 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
7681 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
7741 accatgttat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
7801 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
7861 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
7921 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
7981 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
8041 ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca
8101 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
8161 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
8221 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
8281 aatttcacac aggaaacagc tatgaccatg attacgaatt cgagctcggt acccggggat
8341 cctctagagt cgacctgcag gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt
8401 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
8461 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
8521 aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt
8581 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
8641 tagaataacg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
8701 catgccaacc acagggttcc cctcgggatc aa
//
