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LOCUS pGen2.1 5774 bp ds-DNA circular SYN 23-FEB-2013
DEFINITION CMV promoter-based mammalian expression vector encoding an
N-terminal FLAG tag.
ACCESSION .
VERSION .
KEYWORDS pGen2.1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5774)
AUTHORS GenScript
TITLE Direct Submission
JOURNAL Exported Monday, February 24, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5774
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 917..941
/gene="mutant version of attB"
/bound_moiety="BP Clonase(TM)"
/note="attB1"
/note="recombination site for the Gateway(R) BP reaction"
CDS 948..950
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 951..974
/codon_start=1
/product="FLAG epitope tag, followed by an enterokinase
cleavage site"
/note="FLAG"
/translation="DYKDDDDK"
misc_feature 975..1036
/note="MCS"
/note="multiple cloning site"
protein_bind 1006..1030
/gene="mutant version of attB"
/bound_moiety="BP clonase(TM)"
/note="attB2"
/note="recombination site for the Gateway(R) BP reaction"
promoter complement(1040..1058)
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
polyA_signal 1079..1555
/note="hGH poly(A) signal"
/note="human growth hormone polyadenylation signal"
rep_origin 1644..2072
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2086..2415
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2266..2401
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2482..3276
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3450..3571
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(4022..4607)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4778..5638)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5639..5743)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttacaa gtttgtacaa aaaagcaggc tgccaccatg gattacaagg
961 atgacgacga taaggcggcc gcttgagcat gcatctagag gatccaccca gctttcttgt
1021 acaaagtggt gggccctatt ctatagtgtc acctaaatcg ctagagctcg ctgatcacgg
1081 gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt
1141 gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt
1201 ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg ggatgacaac
1261 ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct
1321 cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt
1381 gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg
1441 tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg
1501 gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttgaaga
1561 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
1621 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
1681 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
1741 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
1801 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
1861 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
1921 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
1981 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
2041 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
2101 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
2161 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
2221 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
2281 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
2341 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
2401 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
2461 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
2521 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
2581 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
2641 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
2701 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
2761 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
2821 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
2881 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
2941 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
3001 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
3061 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
3121 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
3181 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
3241 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
3301 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
3361 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
3421 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
3481 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
3541 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
3601 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
3661 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
3721 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
3781 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
3841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
3901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
3961 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
4021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
4081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
4141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
4201 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
4261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
4321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
4381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
4441 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
4501 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
4561 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
4621 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
4681 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
4741 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
4801 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
4861 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
4921 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
4981 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
5041 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
5101 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
5161 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
5221 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
5281 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
5341 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
5401 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
5461 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
5521 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
5581 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
5641 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
5701 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
5761 tgccacctga cgtc
//
