出品公司: | Clontech |
---|---|
载体名称: | pLVX-rHom-Sec1 |
质粒类型: | 慢病毒载体;双顺反子载体;信号通路载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 9550 bp |
5' 测序引物及序列: | CMV Forward: CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | 嘌呤霉素(Puromycin) |
克隆菌株: | Stbl3 |
宿主细胞(系): | 常规细胞系(293、CV-1、CHO等) |
备注: |
慢毒载体pLVX-rHom-Sec1载体表达DmrD融合蛋白; 利用信号受体的可逆二聚化作用研究细胞信号通路; DmrD的N端带有信号肽。 |
产品目录号: | 635077 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 慢病毒 |
买家导航
pLVX-rHom-Sec1载体质粒基本信息
pLVX-rHom-Sec1质粒图谱载体图谱和pLVX-rHom-Sec1载体序列质粒序列多克隆位点信息
pLVX-rHom-Sec1质粒载体简介
pLVX-rHom-Sec1质粒序列载体序列
LOCUS pLVX-rHom-sec1 9550 bp DNA circular 10-NOV-2011 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Catalog Nos. 635077, 635076 FEATURES Location/Qualifiers LTR 1..635 /label=5'\LTR misc_feature 685..822 /label=packaging\signal misc_feature 2028..2151 /label=CTS/cppt\(DNA\flap) promoter 2185..2788 /label=pCMV misc_feature 5472..6063 /label=WPRE rep_origin complement(7373..8043) /label=pUC\origin CDS complement(8188..9184) /label=Amp(R) misc_binding 1303..1536 /label=RRE LTR 6267..6903 /label=3'\LTR misc_feature 4251..4826 /label=IRES CDS 4859..5458 /label=Puro(R) misc_feature 2822..2899 /label=Secretory\signal\sequence CDS 2903..3226 /label=DmrD CDS 3557..3880 /label=DmrD CDS 3230..3553 /label=DmrD CDS 3884..4207 /label=DmrD BASE COUNT 2438 a 2396 c 2500 g 2216 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tagctagagg atctatttcc ggtgaattcc tcgagactag 2821 tatggctaca ggctcccgga cgtccctgct cctggctttt ggcctgctct gcctgccctg 2881 gcttcaagag ggcagtgcct ctagaggagt gcaggtggag actatctccc caggagacgg 2941 gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga 3001 tggaaagaaa atggattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa 3061 gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc 3121 caaactgact atatctccag attatgccta tggtgccact gggcacccag gcatcatccc 3181 accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaaacta gaggagtgca 3241 ggtggagact atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt 3301 ggtgcactac accgggatgc ttgaagatgg aaagaaaatg gattcctccc gggacagaaa 3361 caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt 3421 tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg 3481 tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct 3541 tctaaaactg gaaactagag gagtgcaggt ggagactatc tccccaggag acgggcgcac 3601 cttccccaag cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa 3661 gaaaatggat tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga 3721 ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact 3781 gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca 3841 tgccactctc gtcttcgatg tggagcttct aaaactggaa actagaggag tgcaggtgga 3901 gactatctcc ccaggagacg ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca 3961 ctacaccggg atgcttgaag atggaaagaa aatggattcc tcccgggaca gaaacaagcc 4021 ctttaagttt atgctaggca agcaggaggt gatccgaggc tgggaagaag gggttgccca 4081 gatgagtgtg ggtcagagag ccaaactgac tatatctcca gattatgcct atggtgccac 4141 tgggcaccca ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg agcttctaaa 4201 actggaaact agtgctagaa accccagcgg ccgcggatcc tacgtatgac cgcccctctc 4261 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg 4321 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg 4381 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag 4441 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt 4501 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc 4561 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga 4621 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga 4681 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct 4741 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg 4801 ttttcctttg aaaaacacga tgataagctt gccacaaccc acaaggagac gaccttccat 4861 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg 4921 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg 4981 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 5041 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 5101 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 5161 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 5221 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 5281 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 5341 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 5401 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctagac 5461 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 5521 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 5581 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 5641 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 5701 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 5761 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 5821 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc 5881 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 5941 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 6001 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 6061 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac 6121 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt 6181 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta 6241 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag 6301 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca 6361 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag 6421 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg 6481 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc 6541 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat 6601 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg 6661 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact 6721 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 6781 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 6841 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 6901 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 6961 gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc 7021 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 7081 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 7141 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 7201 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 7261 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 7321 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 7381 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 7441 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 7501 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 7561 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 7621 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 7681 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 7741 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 7801 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 7861 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 7921 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg 7981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 8041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 8101 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 8161 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 8221 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 8281 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 8341 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 8401 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 8461 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 8521 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 8581 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 8641 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 8701 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 8761 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 8821 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 8881 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 8941 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 9001 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 9061 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 9121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 9181 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca 9241 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 9301 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa 9361 atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat 9421 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa 9481 atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata tgtactacaa 9541 acttagtagt //
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