出品公司: | Clontech |
---|---|
载体名称: | pLVX-rHom-Nuc1 |
质粒类型: | 慢病毒载体;双顺反子载体;信号通路载体;亚细胞定位载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 9160 bp |
5' 测序引物及序列: | CMV Forward: CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | 嘌呤霉素(Puromycin) |
克隆菌株: | Stbl3 |
宿主细胞(系): | 常规细胞系(293、CV-1、CHO等) |
备注: |
慢毒载体pLVX-rHom-Nuc1载体表达DmrD融合蛋白; 利用信号受体的可逆二聚化作用研究细胞信号通路; DmrDN端带有核定位信号序列。 |
产品目录号: | 635077 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 慢病毒 |
买家导航
pLVX-rHom-Nuc1载体质粒基本信息
pLVX-rHom-Nuc1质粒图谱载体图谱和pLVX-rHom-Nuc1载体序列质粒序列多克隆位点信息
pLVX-rHom-Nuc1质粒载体简介
pLVX-rHom-Nuc1质粒序列载体序列
LOCUS pLVX-rHom-Nuc1 9160 bp DNA circular 10-NOV-2011 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Catalog Nos. 635077, 635076 FEATURES Location/Qualifiers LTR 1..635 /label=5'\LTR misc_feature 685..822 /label=packaging\signal misc_feature 2028..2151 /label=CTS/cppt\(DNA\flap) promoter 2185..2788 /label=pCMV misc_feature 5082..5673 /label=WPRE rep_origin complement(6983..7653) /label=pUC\origin CDS complement(7798..8794) /label=Amp(R) misc_binding 1303..1536 /label=RRE LTR 5877..6513 /label=3'\LTR misc_feature 3861..4436 /label=IRES CDS 4469..5068 /label=Puro(R) misc_feature 2822..2845 /label=NLS CDS 2849..3172 /label=DmrD CDS 3176..3499 /label=DmrD CDS 3503..3826 /label=DmrD BASE COUNT 2353 a 2281 c 2388 g 2138 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tagctagagg atctatttcc ggtgaattcc tcgagactag 2821 tatgcctaag aagaagagaa aggtgtctag aggagtgcag gtggagacta tctccccagg 2881 agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct 2941 tgaagatgga aagaaaatgg attcctcccg ggacagaaac aagcccttta agtttatgct 3001 aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca 3061 gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat 3121 catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaactgg aaactagagg 3181 agtgcaggtg gagactatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac 3241 ctgcgtggtg cactacaccg ggatgcttga agatggaaag aaaatggatt cctcccggga 3301 cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga 3361 aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc 3421 ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt 3481 ggagcttcta aaactggaaa ctagaggagt gcaggtggag actatctccc caggagacgg 3541 gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga 3601 tggaaagaaa atggattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa 3661 gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc 3721 caaactgact atatctccag attatgccta tggtgccact gggcacccag gcatcatccc 3781 accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaaacta gttatagcgg 3841 ccgcggatcc tacgtatgac cgcccctctc cctccccccc ccctaacgtt actggccgaa 3901 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 3961 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 4021 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 4081 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 4141 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 4201 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 4261 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 4321 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 4381 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt 4441 gccacaaccc acaaggagac gaccttccat gaccgagtac aagcccacgg tgcgcctcgc 4501 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 4561 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga 4621 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 4681 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 4741 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 4801 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 4861 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 4921 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 4981 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 5041 gtgcatgacc cgcaagcccg gtgcctagac gcgtctggaa caatcaacct ctggattaca 5101 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 5161 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 5221 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 5281 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 5341 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 5401 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 5461 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 5521 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 5581 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 5641 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga 5701 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct 5761 agaagcacaa gaggaggagg aggtgggttt ttccagtcac acctcaggta cctttaagac 5821 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg 5881 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac 5941 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga 6001 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata 6061 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga 6121 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc 6181 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg 6241 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct 6301 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc 6361 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 6421 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 6481 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 6541 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt 6601 tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 6661 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 6721 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 6781 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 6841 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 6901 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 6961 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 7021 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 7081 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 7141 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 7201 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 7261 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 7321 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7381 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7441 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 7501 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7561 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 7621 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 7681 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 7741 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 7801 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 7861 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 7921 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 7981 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 8041 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 8101 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 8161 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 8221 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 8281 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 8341 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 8401 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 8461 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 8521 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 8581 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 8641 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 8701 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 8761 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta 8821 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 8881 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 8941 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta 9001 ttaccactgc caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta 9061 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa 9121 gaaattgtat ttgttaaata tgtactacaa acttagtagt //
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