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pTRE3G-Luc载体质粒基本信息
pTRE3G-Luc质粒图谱载体图谱和pTRE3G-Luc载体序列质粒序列多克隆位点信息
pTRE3G-Luc质粒载体简介
pTRE3G-Luc质粒序列载体序列
LOCUS pTRE3G-Luc 5075 bp ds-DNA circular SYN 11-MAR-2023
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5075)
AUTHORS biofeng.com
TITLE Direct Submission
JOURNAL Exported Saturday, Mar 11, 2023 from SnapGene 4.3.6 to Vector NTI(R)
format
https://www.biofeng.com
COMMENT VNTDATE|1015632000|
COMMENT VNTDBDATE|1015632000|
COMMENT VNTNAME|pTRE3G-Luc.dna|
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
promoter 4..382
/label=TRE3G promoter
/note="3rd-generation Tet-responsive promoter that can be
activated by binding of Tet-On(R) 3G"
protein_bind 12..30
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 48..66
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 84..102
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 120..138
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 156..174
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 192..210
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 228..246
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
CDS 432..2084
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
intron 2227..2292
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 2422..2442
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
polyA_signal 2867..2988
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3245..3833)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4004..4864)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
promoter complement(4865..4969)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctcgagttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga
61 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
121 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
181 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
241 tagagaacgt ataagcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta
301 gtgaaccgtc agatcgcctg gagcaattcc acaacacttt tgtcttatac caactttccg
361 taccacttcc taccctcgta aagtcgacac cggggcccag atctatcgat cggccggata
421 tcacgcgtac catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc
481 tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc
541 ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac gcggaatact
601 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca
661 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat
721 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca
781 gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg caaaaaattt
841 tgaacgtgca aaaaaaatta ccaataatcc agaaaattat tatcatggat tctaaaacgg
901 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta
961 atgaatacga ttttgtacca gagtcctttg atcgtgacaa aacaattgca ctgataatga
1021 actcctctgg atctactggg ttacctaagg gtgtggccct tccgcataga actgcctgcg
1081 tcagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga
1141 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga
1201 tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ttacgatccc
1261 ttcaggatta caaaattcaa agtgcgttgc tagtaccaac cctattttca ttcttcgcca
1321 aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct tctgggggcg
1381 cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt ccagggatac
1441 gacaaggata tgggctcact gagactacat cagctattct gattacaccc gagggggatg
1501 ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg
1561 ataccgggaa aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga ggacctatga
1621 ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat
1681 ggctacattc tggagacata gcttactggg acgaagacga acacttcttc atagttgacc
1741 gcttgaagtc tttaattaaa tacaaaggat accaggtggc ccccgctgaa ttggagtcga
1801 tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtcttccc gacgatgacg
1861 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag
1921 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg
1981 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag
2041 agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaactgcag ggatccaatg
2101 taactgtatt cagcgatgac gaaattctta gctattgtaa tactctagag gatctttgtg
2161 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
2221 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
2281 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
2341 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
2401 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
2461 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
2521 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
2581 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
2641 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
2701 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
2761 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
2821 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
2881 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
2941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgcgg
3001 ctctagagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
3061 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
3121 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
3181 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
3241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
3301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
3361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
3421 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
3481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
3541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
3601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
3661 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
3721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
3781 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
3841 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
3901 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
3961 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
4021 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
4081 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
4141 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
4201 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
4261 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
4321 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
4381 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
4441 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
4501 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
4561 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
4621 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
4681 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
4741 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
4801 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
4861 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
4921 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
4981 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
5041 ggcgtatcac gaggcccttt cgtcttcaag aattc
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