出品公司: | -- |
---|---|
载体名称: | pCDNA3.0-HA-N |
质粒类型: | 哺乳细胞真核表达载体;蛋白过表达质粒 |
终止子: | bGH poly(A) signal |
复制子: | pUC |
启动子: | CMV |
载体大小: | 5484 bp |
5' 测序引物及序列: | T7:TAATACGACTCACTATAGGG |
3' 测序引物及序列: | SP6:ATTTAGGTGACACTATAGAA |
载体标签: | N-HA |
载体抗性: | 氨苄青霉素 |
筛选标记: | G418 |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 哺乳动物 |
备注: | 载体上有起始密码子ATG,注意不要移码。 |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | -- |
病毒/非病毒: | -- |
买家导航
pCDNA3.0-HA-N载体质粒基本信息
pCDNA3.0-HA-N质粒图谱载体图谱和pCDNA3.0-HA-N载体序列质粒序列多克隆位点信息
pCDNA3.0-HA-N质粒载体简介
pCDNA3.0-HA-N质粒序列载体序列
LOCUS pCDNA3.0-HA-N 5484 bp ds-DNA circular SYN 20-NOV-2016 DEFINITION synthetic circular DNA http://www.biofeng.com/ FEATURES Location/Qualifiers source 1..5484 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 235..614 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" CDS 898..924 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /translation="YPYDVPDYA" misc_feature 927..1032 /note="MCS" promoter complement(1036..1054) /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" polyA_signal 1080..1304 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" rep_origin 1350..1778 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1792..2122 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 1973..2108 /note="SV40 ori" /note="SV40 origin of replication" CDS 2189..2983 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3157..3278 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" primer_bind complement(3327..3343) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 3351..3367 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3375..3405) /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 3420..3441 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3729..4317) /direction=LEFT /note="pUC ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4488..5348) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5349..5453) /gene="bla" /note="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccgc caccatgtac 901 ccatacgacg tcccagacta cgctggaagc ttggtaccga gctcggatcc actagtaacg 961 gccgccagtg tgctggaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca 1021 tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac 1081 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct 1141 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct 1201 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg 1261 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag 1321 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc 1381 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 1441 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 1501 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 1561 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 1621 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 1681 ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt 1741 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag 1801 ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct 1861 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 1921 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 1981 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 2041 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 2101 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 2161 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 2221 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 2281 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 2341 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 2401 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 2461 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 2521 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 2581 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 2641 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 2701 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 2761 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 2821 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 2881 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 2941 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 3001 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 3061 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 3121 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat 3181 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 3241 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc 3301 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 3361 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 3421 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 3481 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 3541 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 3601 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 3661 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 3721 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 3781 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 3841 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 3901 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc 3961 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 4021 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 4081 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 4141 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 4201 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 4261 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 4321 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 4381 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 4441 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 4501 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 4561 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 4621 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 4681 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 4741 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 4801 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 4861 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 4921 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 4981 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 5041 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 5101 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 5161 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 5221 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 5281 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 5341 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 5401 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 5461 ccccgaaaag tgccacctga cgtc //
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pCDNA3.0-HA-N质粒载体应用举例
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