质粒名称: | DsRed2-Mito-7 |
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出品公司: | Addgene |
目录编号: | 55838 |
存储实验室: | Michael Davidson |
载体骨架基本信息 |
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载体名称: | DsRed2 |
载体出品公司: | Clontech |
空载体大小: | 4750bp |
完整质粒大小: | 4715bp |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
载体抗性: | Kanamycin |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 高拷贝 |
插入基因信息 |
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插入基因名称: | Mito、NM_004074.2 |
物种来源: | H. sapiens (human) |
GenBank编号 | JN247409 |
启动子: | CMV |
标签/融合蛋白: | DsRed2 (C terminal on backbone) |
克隆方法: | 未知 |
5’测序引物 | CMV-F |
3’测序引物 | SV40pA-R |
文献引用方法 |
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材料和方法部分: | DsRed2-Mito-7 was a gift from Michael Davidson (Addgene plasmid # 55838 ; http://n2t.net/addgene:55838 ; RRID:Addgene_55838) |
买家导航
DsRed2-Mito-7载体质粒基本信息
DsRed2-Mito-7质粒图谱载体图谱和DsRed2-Mito-7载体序列质粒序列多克隆位点信息
DsRed2-Mito-7质粒载体简介
DsRed2-Mito-7质粒序列载体序列
LOCUS DsRed2-Mito-7 4715 bp DNA circular SYN 25-AUG-2024 DEFINITION Localization: Mitochondria, Excitation: 558, Emission: 583. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4715) TITLE Davidson Red fluorescent proteins REFERENCE 2 (bases 1 to 4715) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3 https://www.biofeng.com COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4715 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 64..367 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 368..571 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 522..542 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 600..674 /codon_start=1 /product="mitochondrial presequence of human cytochrome c oxidase subunit VIII (Rizutto et al., 1989)" /label=COX8 presequence /translation="MSVLTPLLLRGLTGSARRLPVPRAK" regulatory 702..711 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" CDS 708..1385 /codon_start=1 /product="improved tetrameric variant of DsRed fluorescent protein" /label=DsRed2 /note="mammalian codon-optimized" /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH LFL" primer_bind complement(888..908) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 1300..1323 /label=DsRed1-C /note="DsRed1, forward primer" polyA_signal 1506..1627 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1543..1562) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1597..1616 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1634..2089) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1771..1792) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1983..2002 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2116..2220 /gene="bla" /label=AmpR promoter promoter 2222..2579 /label=SV40 promoter /note="SV40 enhancer and early promoter" enhancer 2222..2413 /label=SV40 enhancer /note="enhancer for the SV40 early promoter (Herr, 1993)" primer_bind complement(2245..2265) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2430..2565 /label=SV40 ori /note="SV40 origin of replication" CDS 2614..3408 /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" CDS 2614..3408 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2668..2687) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3278..3297 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3596..3615) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3640..3687 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4016..4604 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4505..4524 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 61 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 361 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg 421 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca 481 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg 541 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagca 601 tgtccgtcct gacgccgctg ctgctgcggg gcttgacagg ctcggcccgg cggctcccag 661 tgccgcgcgc caagatccat tcgttggggg atccaccggt cgccaccatg gcctcctccg 721 agaacgtcat caccgagttc atgcgcttca aggtgcgcat ggagggcacc gtgaacggcc 781 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac aacaccgtga 841 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt 901 tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac tacaagaagc 961 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg 1021 cgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag gtgaagttca 1081 tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg ggctgggagg 1141 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc cacaaggccc 1201 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac atggccaaga 1261 agcccgtgca gctgcccggc tactactacg tggacgccaa gctggacatc acctcccaca 1321 acgaggacta caccatcgtg gagcagtacg agcgcaccga gggccgccac cacctgttcc 1381 tgtagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg 1441 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 1501 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 1561 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 1621 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 1681 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 1741 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 1801 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 1861 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 1921 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 1981 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 2041 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact 2101 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 2161 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 2221 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 2281 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 2341 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 2401 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 2461 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 2521 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag 2581 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 2641 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 2701 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 2761 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg 2821 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 2881 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 2941 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 3001 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 3061 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 3121 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc 3181 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 3241 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 3301 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 3361 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 3421 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg 3481 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 3541 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa 3601 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata 3661 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact 3721 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc 3781 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag 3841 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt 3901 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 3961 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 4021 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 4081 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 4141 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 4201 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 4261 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 4321 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 4381 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 4441 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 4501 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 4561 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 4621 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 4681 cccctgattc tgtggataac cgtattaccg ccatg //
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DsRed2-Mito-7质粒载体应用举例
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