PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

LOCUS        PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry                9238 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  PPI reporter.
ACCESSION   .
VERSION     .
KEYWORDS    PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Zhang Q, Zheng YW, Coughlin SR, Shu X
  TITLE     A rapid fluorogenic GPCR-beta-arrestin interaction assay.
  JOURNAL   Protein Sci. 2018 Apr;27(4):874-879. doi: 10.1002/pro.3385. Epub 
            2018 Feb 23.
  PUBMED    29411438
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2020..2073
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2080..2089
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2176..2196
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2230..2499
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             3793..3846
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3853..4563
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3998..4016)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4266..4285
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4595..4612)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4601..4825
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4871..5299
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4958..4977)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5168..5189
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5308..5328)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5313..5642
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5493..5628
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5555..5574
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5709..6503
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5763..5782)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6373..6392
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6677..6798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6714..6733)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6768..6787
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6847..6863)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6847..6863)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6860..6882)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6871..6887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6895..6925)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6940..6961
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7078..7095)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7249..7837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7329..7348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8008..8868)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8631..8650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8869..8973)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9048..9067)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttccaccatg gataacgtcc ttcctatgga ctctgatctc
      721 ttccccaaca tctccaccaa cacctctgag tctaaccagt tcgtgcagcc tacctggcaa
      781 atcgttcttt gggcggctgc ctacacggtc atcgtggtga cttccgtggt gggcaacgta
      841 gtggtgatat ggatcatttt ggcccacaag agaatgagga cagtgaccaa ttatttcctg
      901 gtgaacctgg ccttcgctga ggcctgcatg gctgcattca atacggtggt gaacttcacc
      961 tacgcagtcc acaatgtgtg gtactacggc ctcttctatt gcaagtttca caacttcttc
     1021 cccatcgctg ctctcttcgc cagtatctac tccatgacag ccgtggcctt cgacagatac
     1081 atggccatca tccaccctct ccagccccgg ctctcagcca cggctaccaa agtggtcatc
     1141 tttgtcatct gggtcctggc tctcctgctg gcctttccac aaggctacta ctccaccaca
     1201 gagaccatgc ccagcagagt cgtgtgcatg atcgagtggc cggagcatcc caacaggact
     1261 tatgagaaag cgtaccacat ctgcgtgact gtactgatct acttcctgcc tctactggtg
     1321 atcggctacg cgtacactgt ggtggggatt acactgtggg caagcgagat ccccggcgac
     1381 tcctctgacc gctaccatga gcaagtctct gccaaacgca aggtggtcaa aatgacgatc
     1441 gtggttgtgt gtaccttcgc catctgctgg ctgcccttcc acgtcttctt cctcctgccc
     1501 tacatcaacc cagatcccca cctgaagaag ttcatccagc aggtctacct ggccagcatg
     1561 tggctggcca tgagttctac catgtacaac cccatcatct actgttgcct caacgacagg
     1621 ttccgtctgg gcttcaagca cgccttccgc tgctgcccct tcatcagtgc aggtgattat
     1681 gaggggctgg aaatgaaatc cacccgatac ctccagacac agagcagcgt gtacaaggtc
     1741 agccgcctgg agaccaccat ctccactgtg gtgggagccc atgaagagga gcccgaggaa
     1801 ggccccaagg ccacaccctc atccttggat ctcacctcca acggctcctc tcgcagcaac
     1861 tccaagacca tgacagaaag ctccagcttc tactctaaca tgctggcctt aattaactct
     1921 ggtggcggga gtggtggcgg gggtaccatg gaaaagcgag accatatggt tttgcttgag
     1981 tatgttacag cggctggcat taccgatgca tcaggatccg agggcagagg aagtctgcta
     2041 acatgcggtg acgtcgagga gaatcctggc ccagaattcg ccaccatgga cctgcctgac
     2101 gaccactacc tgtccaccca gaccatcctg tccaaggacc tgaactccgg actcagatct
     2161 ggcagcggtg gaggcgaaaa tctctacttc cagagtagcg catccggcgg aagcggaagc
     2221 ctcgagatgg agatgtggca cgagggactc gaagaggcca gcaggctgta ctttggcgag
     2281 aggaacgtca agggcatgtt cgaagtgctg gagcccctcc atgcgatgat ggaaaggggc
     2341 ccacagaccc tgaaggagac cagcttcaac caggcttacg gcagggacct gatggaggca
     2401 caggaatggt gcaggaagta catgaagagc ggcaacgtga aagacctgac ccaggcgtgg
     2461 gacctctact accacgtgtt caggaggatc agcaagcagg gcggaggagg cagcgggcgc
     2521 gccatgggcg acaaagggac acgagtgttc aagaaggcaa gccccaatgg aaagctcact
     2581 gtctacctgg gaaagcggga ctttgtggac cacattgacc tcgtggaccc cgtggatggt
     2641 gtggtcctgg tggatcctga gtatctcaaa gaaaggcgag tctacgtgac actgacctgc
     2701 gccttccggt atggccggga agacctggat gtcttgggtc tgacttttcg gaaagacctg
     2761 tttgtggcca atgtgcagtc cttcccaccg gcccccgagg acaagaagcc actgacacgg
     2821 ctacaggagc gactcatcaa gaagctgggc gagcatgcct gccccttcac ctttgagatc
     2881 ccgccaaacc ttccatgctc agtcactctg cagcctgggc ctgaggacac aggaaaggcc
     2941 tgtggtgtgg attatgaagt caaagccttc tgtgctgaga acctggagga gaagatccac
     3001 aaaaggaact ctgtgcggct agtcatccgg aaggttcagt atgcccctga gagacctggc
     3061 cctcagccca cggctgagac taccagacag ttccttatgt cagacaagcc cctacacctt
     3121 gaggcatcac tggataagga gatctattac cacggagaac ccatcagcgt taatgtccac
     3181 gtcaccaaca acaccaacaa gactgtgaag aagatcaaga tctcggtgcg ccagtatgca
     3241 gacatctgtc tcttcaacac agctcagtac aagtgcccag tggccatgga ggaggctgat
     3301 gacaatgtgg cacccagctc aacattctgc aaggtctata cgctgactcc cttcctggcc
     3361 aacaaccgag agaagcgggg actcgccctc gacgggaagc tcaagcatga ggacacgaat
     3421 ctggcttcca gcacgctgtt gcgggaaggt gccaaccgtg aaatcctggg catcatcgtt
     3481 tcctacaaag tcaaggtgaa actggtggtg tcccggggcg gcctcttggg agaccttgca
     3541 tccagtgatg tggccgtgga gctgcctttt accctaatgc accccaagcc taaggaggag
     3601 cccccacatc gggaagttcc agagagcgag actccagtag acaccaatct catagagctt
     3661 gacaccaatg atgacgacat tgtatttgag gactttgctc gccagcggct gaaaggcatg
     3721 aaggatgaca aggacgaaga ggatgacggc accggctctc cgcacctcaa caacagaggc
     3781 ggccgctcta gagagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct
     3841 ggcccagaat tgatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     3901 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     3961 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     4021 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     4081 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     4141 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     4201 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     4261 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     4321 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     4381 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     4441 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     4501 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     4561 taatctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     4621 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4681 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4741 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4801 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     4861 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4921 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4981 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5041 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5101 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5161 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5221 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5281 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     5341 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     5401 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5461 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5521 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5581 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5641 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     5701 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5761 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5821 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5881 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5941 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6001 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6061 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6121 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6181 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     6241 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6301 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6361 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6421 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6481 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     6541 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6601 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     6661 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6721 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6781 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     6841 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6901 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6961 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7021 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7081 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7141 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7201 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7261 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7321 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7381 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7441 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7501 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7561 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7621 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7681 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7741 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7801 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7861 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7921 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7981 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8041 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8101 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8161 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8221 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8281 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8341 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8401 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8461 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8521 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8581 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8641 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8701 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8761 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8821 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8881 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8941 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9001 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     9061 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     9121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     9181 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//