| 质粒名称: | pJH-MBP-CbAgo |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 127707 |
| 存储实验室: | John van der Oost |
|
载体骨架基本信息 |
|
| 载体名称: | pET (Plasmid #29656) |
| 载体出品公司: | Scott Gradia |
| 空载体大小: | 6472bp |
| 完整质粒大小: | 8716 bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | CbAgo |
| 物种来源: | Clostridium butyricum |
| 插入大小: | 2244bp |
| 启动子: | T7 |
| 标签/融合蛋白: | MPB (N terminal on insert) |
| 克隆方法: | Ligation Independent Cloning |
| 5’克隆位点: | AgeI(未破坏) |
| 5’测序引物 | TAATACGACTCACTATAGGG |
| 3’测序引物 | GCTAGTTATTGCTCAGCGG |
|
文献引用方法 |
|
| 材料和方法部分: | pJH-MBP-CbAgo was a gift from John van der Oost (Addgene plasmid # 127707 ; http://n2t.net/addgene:127707 ; RRID:Addgene_127707) |
| 参考文献部分: | DNA-guided DNA cleavage at moderate temperatures by Clostridium butyricum Argonaute. Hegge JW, Swarts DC, Chandradoss SD, Cui TJ, Kneppers J, Jinek M, Joo C, van der Oost J. Nucleic Acids Res. 2019 May 9. pii: 5487266. doi: 10.1093/nar/gkz306. 10.1093/nar/gkz306 PubMed 31069393 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJH-MBP-CbAgo 8713 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION pET His6 MBP TEV CbAgo.
ACCESSION .
VERSION .
KEYWORDS pJH-MBP-CbAgo
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8713)
AUTHORS Hegge JW, Swarts DC, Chandradoss SD, Cui TJ, Kneppers J, Jinek M,
Joo C, van der Oost J
TITLE DNA-guided DNA cleavage at moderate temperatures by Clostridium
butyricum Argonaute.
JOURNAL Nucleic Acids Res. 2019 May 9. pii: 5487266. doi:
10.1093/nar/gkz306.
PUBMED 31069393
REFERENCE 2 (bases 1 to 8713)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8713
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 106..125
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 209..228
/label=T7
/note="T7 promoter, forward primer"
promoter 209..227
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 228..252
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 267..289
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 308..325
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 335..1435
/codon_start=1
/gene="malE (mutated)"
/product="maltose binding protein from E. coli"
/label=MBP
/note="This version of the gene does not encode a signal
sequence, so MBP will remain in the cytosol."
/translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
WYAVRTAVINAASGRQTVDEALKDAQT"
primer_bind 1409..1432
/label=MBP-F
/note="Maltose binding protein, forward primer"
CDS 1448..1468
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQS"
CDS 3785..3802
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(3855..3873)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 3869..3916
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 3953..4408
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4040..4059)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4250..4271
/label=F1ori-F
/note="F1 origin, forward primer"
CDS complement(4501..5316)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 5224..5243
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 5308..5327
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 5438..6026
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5927..5946
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6180..6197
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 6212..6351
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(6277..6296)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6437..6459
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(6453..6644)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(7453..8535)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 8497..8516
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(8536..8613)
/gene="lacI"
/label=lacI promoter
ORIGIN
1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg
241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat ataccatggg
301 ttcttctcac catcaccatc accatggttc ttctatgaaa atcgaagaag gtaaactggt
361 aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta agaaattcga
421 gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag agaaattccc
481 acaggttgcg gcaactggcg atggccctga cattatcttc tgggcacacg accgctttgg
541 tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt tccaggacaa
601 gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg cttacccgat
661 cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc cgccaaaaac
721 ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga gcgcgctgat
781 gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg ggggttatgc
841 gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata acgctggcgc
901 gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga atgcagacac
961 cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg
1021 cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa cggtactgcc
1081 gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc
1141 cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa aactatctgc tgactgatga
1201 aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga agtcttacga
1261 ggaagagttg gcgaaagatc cacgtattgc cgccactatg gaaaacgccc agaaaggtga
1321 aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtgcgta ctgcggtgat
1381 caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc agactaatgg
1441 gatcgaggaa aacctgtact tccaatccaa tgcaaataat ctgacctttg aggcttttga
1501 agggattggt caactgaatg aactgaattt ttataagtat cgtctgattg ggaaagggca
1561 gattgataat gtgcatcaag ctatttggtc tgttaaatat aaactccagg ctaataattt
1621 ttttaaaccg gtgtttgtga aaggtgagat tctatatagc ctagatgaac tgaaggtgat
1681 tccggaattt gaaaatgttg aggtgattct cgatggtaat attattctgt ctatttctga
1741 gaataccgat atttataagg atgtgattgt gttctatatt aacaatgctc tcaaaaatat
1801 taaagatatt actaattatc gtaagtacat taccaaaaat accgatgaga ttatatgtaa
1861 atctattctg actaccaatc tgaaatatca gtatatgaaa tctgaaaaag ggtttaaact
1921 gcagaggaaa tttaaaatta gcccggttgt ttttcgtaat gggaaggtga ttctgtatct
1981 caattgtagc agcgattttt ctaccgataa gtctatatat gagatgctaa atgatggtct
2041 gggggtggtg ggtctacaag tgaaaaataa atggaccaat gcaaatggga atattttcat
2101 tgaaaaagta ctggataata ccatttctga cccgggtact tctggtaagc taggtcagtc
2161 tctaattgat tactatatta atggtaatca aaagtatcgt gttgaaaagt ttaccgatga
2221 agataaaaat gcaaaggtga ttcaggctaa gattaagaat aaaacctata attatattcc
2281 gcaagctctg accccggtga ttaccaggga atatctgagc cataccgata agaagttttc
2341 taagcagatt gagaatgtga ttaaaatgga catgaattat cgttatcaga ccctaaaatc
2401 tttcgttgaa gatattggtg tgattaaaga actcaataat ctgcatttta aaaatcagta
2461 ttatactaat tttgatttta tggggtttga atctggtgtt ctggaagaac cggtactgat
2521 gggggctaac ggtaaaatta aagataagaa gcaaattttt attaatgggt ttttcaagaa
2581 tccgaaggag aatgtgaagt ttggtgttct gtatccggaa gggtgtatgg aaaatgctca
2641 gtcgattgct cgttctatac tagattttgc aaccgctggg aaatataata aacaagagaa
2701 taaatatatt agcaagaatc taatgaatat tggttttaag ccgtctgaat gtattttcga
2761 atcttataaa ctcggtgata ttaccgaata taaagcaacc gctcgtaagc taaaagaaca
2821 tgaaaaggtg ggttttgtga ttgcagtgat tccggatatg aatgaactgg aagtggaaaa
2881 tccgtataat ccgtttaaaa aagtatgggc aaaactgaat attccgagcc agatgattac
2941 cctgaaaacc accgaaaaat ttaaaaatat tgttgataag tctggtctat attatctaca
3001 caatattgct ctcaatattc tagggaaaat tggggggatt ccgtggatta ttaaagatat
3061 gccgggtaat attgattgtt tcattgggct agatgtgggt acccgtgaaa aaggtattca
3121 ttttccggca tgtagcgttc tatttgataa atatgggaaa ctgattaatt attataagcc
3181 gaccattccg cagtctggtg agaaaattgc agaaaccatt ctgcaagaga ttttcgataa
3241 tgttctgatt agctataaag aagaaaatgg ggaatatccg aaaaatattg tgattcatcg
3301 tgatgggttt tctcgtgaaa atattgattg gtataaagaa tattttgata agaaagggat
3361 taaatttaac attattgagg tgaaaaagaa tattccggtt aaaattgcaa aggtggttgg
3421 gtcgaacatt tgtaatccca ttaaaggttc ttatgttctc aaaaatgata aggcttttat
3481 tgttaccacc gatattaaag atggggttgc tagcccgaac cccctgaaaa ttgaaaagac
3541 ctatggtgac gtggagatga aaagcattct agaacagatt tatagcctgt ctcaaattca
3601 tgttgggagc accaagtctc taaggctccc gattaccacc ggttatgctg ataaaatttg
3661 caaagcaatt gagtacattc cgcaaggtgt tgttgataat cgtctattct ttctgtaata
3721 acattggaag tggataacgg atccgaattc gagcgccgtc gacaagcttg cggccgcact
3781 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
3841 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
3901 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
3961 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
4021 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
4081 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
4141 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
4201 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
4261 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
4321 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
4381 tttaacaaac tagtaacgtt tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga
4441 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc
4501 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
4561 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
4621 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
4681 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
4741 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
4801 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
4861 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
4921 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaatg ttttcacctg aatcaggata
4981 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
5041 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
5101 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
5161 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
5221 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
5281 cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
5341 gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact
5401 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
5461 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
5521 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
5581 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
5641 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
5701 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
5761 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
5821 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
5881 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
5941 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
6001 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
6061 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
6121 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
6181 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
6241 tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata
6301 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
6361 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
6421 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
6481 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct
6541 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
6601 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
6661 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
6721 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg
6781 gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat
6841 acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata
6901 atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc
6961 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
7021 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc
7081 aacgacagga gcacgatcat gcgcacccgt ggggccgcca tgccggcgat aatggcctgc
7141 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag
7201 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg
7261 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
7321 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
7381 aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta
7441 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
7501 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct
7561 tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg
7621 cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa
7681 cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc
7741 accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt
7801 ggcaaccagc atcgcagtgg gaacgatacc ctcattcagc atttgcatgg tttgttgaaa
7861 accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt
7921 gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc
7981 taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc
8041 gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa
8101 cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata
8161 gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc
8221 ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg
8281 agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac
8341 gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt
8401 cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg
8461 gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa
8521 cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat
8581 accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg
8641 actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
8701 ggaatggtgc atg
//
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