| 出品公司: | -- |
|---|---|
| 载体名称: | pCS2(+) |
| 质粒类型: | 克隆载体；哺乳动物表达载体；广宿主载体；体外表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | simian CMV IE94、lac、T3、SP6 |
| 载体大小: | 4095 bp |
| 5' 测序引物及序列: | SP6 fwd（MCS1）、T3 fwd（MCS2） |
| 3' 测序引物及序列: | EBV rev（MCS1）、SV40 polyA rev（MCS2） |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH10B |
| 表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
| 备注: | pCS2(+)载体能够在多种细胞中进行高水平的瞬表达。pCS2(+)载 体同时也能进行体外转录和翻译。 |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCS2+载体质粒基本信息
pCS2+质粒图谱载体图谱和pCS2+载体序列质粒序列多克隆位点信息
 Map.gif)
 多克隆位点1.gif "pCS2(+) 多克隆位点1 点击看大图")
 多克隆位点2.gif "pCS2(+) 多克隆位点2 点击看大图")
pCS2+质粒载体简介
pCS2+ contains a strong enhancer/promoter (simian CMV IE94) followed by a polylinker and the SV40 late polyadenlyation site. An SP6 promoter is present in the 5' untranslated region of the mRNA from the sCMV promoter, allowing in vitro RNA synthesis of sequences cloned into the polylinker. A T7 promoter in reverse orientation between the polylinker and the SV40 polyA site for probe synthesis, as well as a second polylinker after the SV40 polyA site to provide several possible sites to linearize the vector for SP6 RNA transcription. The vector backbone is from pBluescript II KS+ and includes the amp resistance gene and an f1 origin for producing single stranded DNA.
pCS2+质粒序列载体序列
LOCUS Exported 4095 bp ds-DNA circular SYN 29-JUL-2016
DEFINITION Vector that allows high-level transient expression in vertebrate
cells as well as in vitro transcription/translation.
KEYWORDS pCS2+
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4095)
AUTHORS Biofeng Lab.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pCS2(+)|
FEATURES Location/Qualifiers
source 1..4095
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 35..53
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
misc_feature 79..120
/note="MCS1"
misc_feature 117..144
/note="stop codons"
/note="stop codons in all three reading frames"
polyA_signal 144..278
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
misc_feature 345..378
/note="MCS2"
promoter complement(391..409)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature complement(432..434)
/note="ATG"
protein_bind 454..470
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(478..508)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(832..1420)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1591..2451)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2452..2556)
/gene="bla"
/note="AmpR promoter"
rep_origin complement(2582..3037)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3111..4095
/note="CMV IE94 promoter"
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
ORIGIN
1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcctctcg agcctctaga
121 actatagtga gtcgtattac gtagatccag acatgataag atacattgat gagtttggac
181 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
241 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
301 ttatgtttca ggttcagggg gaggtgtggg aggtttttta attcgcggcc gcggcgccaa
361 tgcattgggc ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
421 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
481 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
541 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
601 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
661 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
721 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
781 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
841 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
901 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
961 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
1021 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
1081 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
1141 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
1201 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
1261 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
1321 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
1381 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
1441 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
1501 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
1561 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
1621 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
1681 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
1741 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
1801 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
1861 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
1921 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
1981 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
2041 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
2101 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
2161 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
2221 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
2281 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
2341 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
2401 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
2461 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
2521 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
2581 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
2641 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
2701 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
2761 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
2821 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
2881 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
2941 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
3001 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
3061 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagtc gaccatagcc
3121 aattcaatat ggcgtatatg gactcatgcc aattcaatat ggtggatctg gacctgtgcc
3181 aattcaatat ggcgtatatg gactcgtgcc aattcaatat ggtggatctg gaccccagcc
3241 aattcaatat ggcggacttg gcaccatgcc aattcaatat ggcggacttg gcactgtgcc
3301 aactggggag gggtctactt ggcacggtgc caagtttgag gaggggtctt ggccctgtgc
3361 caagtccgcc atattgaatt ggcatggtgc caataatggc ggccatattg gctatatgcc
3421 aggatcaata tataggcaat atccaatatg gccctatgcc aatatggcta ttggccaggt
3481 tcaatactat gtattggccc tatgccatat agtattccat atatgggttt tcctattgac
3541 gtagatagcc cctcccaatg ggcggtccca tataccatat atggggcttc ctaataccgc
3601 ccatagccac tcccccattg acgtcaatgg tctctatata tggtctttcc tattgacgtc
3661 atatgggcgg tcctattgac gtatatggcg cctcccccat tgacgtcaat tacggtaaat
3721 ggcccgcctg gctcaatgcc cattgacgtc aataggacca cccaccattg acgtcaatgg
3781 gatggctcat tgcccattca tatccgttct cacgccccct attgacgtca atgacggtaa
3841 atggcccact tggcagtaca tcaatatcta ttaatagtaa cttggcaagt acattactat
3901 tggaaggacg ccagggtaca ttggcagtac tcccattgac gtcaatggcg gtaaatggcc
3961 cgcgatggct gccaagtaca tccccattga cgtcaatggg gaggggcaat gacgcaaatg
4021 ggcgttccat tgacgtaaat gggcggtagg cgtgcctaat gggaggtcta tataagcaat
4081 gctcgtttag ggaac
//
