| 出品公司: | -- |
|---|---|
| 载体名称: | pCS22+ |
| 质粒类型: | 克隆载体；哺乳动物表达载体；广宿主载体；体外表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | simian CMV IE94、lac、T3、SP6 |
| 载体大小: | 4046 bp |
| 5' 测序引物及序列: | SP6 fwd（MCS1）、T3 fwd（MCS2） |
| 3' 测序引物及序列: | EBV rev（MCS1）、SV40 polyA rev（MCS2） |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH10B |
| 表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
| 备注: | pCS22+载体能够在多种细胞中进行高水平的瞬表达。pCS22+载体同时也能进行体外转录和翻译。 |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCS22+载体质粒基本信息
pCS22+质粒图谱载体图谱和pCS22+载体序列质粒序列多克隆位点信息
 Map.gif)
 多克隆位点1.gif "pCS22(+) 多克隆位点1 点击看大图")
 多克隆位点2.gif "pCS22(+) 多克隆位点2 点击看大图")
pCS22+质粒载体简介
pCS22+质粒序列载体序列
LOCUS Exported 4046 bp ds-DNA circular SYN 31-JUL-2016
DEFINITION Vector that allows high-level transient expression in vertebrate
cells as well as in vitro transcription/translation.
KEYWORDS pCS22+
SOURCE Biofeng Lab.
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4046)
AUTHORS I.M.A.G.E. Consortium
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pCS22+|
FEATURES Location/Qualifiers
source 1..4046
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 34..52
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 69..101
/note="MCS1"
promoter complement(103..121)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
polyA_signal 128..262
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
misc_feature 330..362
/note="MCS2"
protein_bind 405..421
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(429..459)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(783..1371)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1542..2402)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2403..2507)
/gene="bla"
/note="AmpR promoter"
rep_origin complement(2533..2988)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3062..4046
/note="CMV IE94 promoter"
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
ORIGIN
1 cgccattctg cctggggacg tcggagcaag ctttaatacg actcactata gggcaataca
61 agctacttgt ttaaactctg cagggatccg aattcctcga gccctttagt gagggttaat
121 ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
181 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
241 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
301 gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta
361 ccgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
421 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
481 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
541 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
601 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
661 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
1021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
1081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
1141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
1201 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
1261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
1321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
1381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
1441 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
1501 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
1561 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
1621 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
1681 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
1741 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
1801 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
1861 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
1921 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
1981 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
2041 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
2101 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
2161 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
2221 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
2281 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
2341 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
2401 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
2461 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
2521 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
2581 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
2641 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
2701 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
2761 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
2821 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
2881 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
2941 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
3001 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt
3061 cgaccatagc caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct
3121 ggacctgtgc caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct
3181 ggaccccagc caattcaata tggcggactt ggcaccatgc caattcaata tggcggactt
3241 ggcactgtgc caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct
3301 tggccctgtg ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt
3361 ggctatatgc caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct
3421 attggccagg ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt
3481 ttcctattga cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt
3541 cctaataccg cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc
3601 ctattgacgt catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa
3661 ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt
3721 gacgtcaatg ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc
3781 aatgacggta aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag
3841 tacattacta ttggaaggac gccagggtac attggcagta ctcccattga cgtcaatggc
3901 ggtaaatggc ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa
3961 tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct
4021 atataagcaa tgctcgttta gggaac
//
